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  4. Rat Stromal cell derived factor 1a,SDF-1a ELISA Kit

Rat Stromal cell derived factor 1a,SDF-1a ELISA Kit

  • 中文名稱：
    大鼠基質(zhì)細胞衍生因子1a(SDF-1a/CXCL12)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08729r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    The Rat Stromal cell-derived factor 1a (SDF-1a) ELISA Kit is a reliable and efficient solution for researchers in the field of immunology. This ELISA kit enables the quantitative measurement of SDF-1a in a range of sample types, including serum, plasma, cell culture supernates, and tissue homogenates from Rattus norvegicus (Rat).

    With a detection range of 0.78 pg/mL to 50 pg/mL and a sensitivity of 0.195 pg/mL, this ELISA kit delivers accurate and precise results, making it a valuable tool for researchers working on SDF-1a in rats. The assay time is only 1-5 hours, and the sample volume required is only 50-100ul, making it a convenient and easy-to-use product.

    This ELISA kit works on a sandwich assay principle, where the SDF-1a antigen is captured by a specific antibody and detected by a second antibody labeled with a detection reagent. The detection wavelength is 450 nm, providing a reliable and consistent readout.

    The Rat SDF-1a ELISA Kit has been cited in more than 4 publications, highlighting its reliability and utility in research. It is an excellent addition to any immunology research laboratory, providing accurate and precise measurement of SDF-1a in rat samples.

  • 縮寫：
    SDF1a/CXCL12
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    0.78 pg/mL-50 pg/mL
  • 靈敏度：
    0.195 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SDF-1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %88
    Range %83-97
    1:200Average %97
    Range %91-103
    1:400Average %84
    Range %80-88
    1:800Average %89
    Range %81-95
  • 回收率：
    The recovery of rat SDF-1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-96
    EDTA plasma (n=4)9794-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    502.830 2.775 2.803 2.666
    252.326 2.453 2.390 2.253
    12.51.600 1.632 1.616 1.479
    6.250.937 0.894 0.916 0.779
    3.120.528 0.532 0.530 0.393
    1.560.321 0.335 0.328 0.191
    0.780.157 0.148 0.153 0.016
    00.140 0.133 0.137 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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