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  4. Recombinant Arabidopsis thaliana Sodium transporter HKT1 (HKT1), partial

Recombinant Arabidopsis thaliana Sodium transporter HKT1 (HKT1), partial

  • 中文名稱：
    擬南芥HKT1重組蛋白
  • 貨號(hào)：
    CSB-YP802924DOA
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥HKT1重組蛋白
  • 貨號(hào)：
    CSB-EP802924DOA
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥HKT1重組蛋白
  • 貨號(hào)：
    CSB-EP802924DOA-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥HKT1重組蛋白
  • 貨號(hào)：
    CSB-BP802924DOA
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥HKT1重組蛋白
  • 貨號(hào)：
    CSB-MP802924DOA
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    HKT1
  • Uniprot No.：
  • 別名：
    HKT1; At4g10310; T9A4.5Sodium transporter HKT1; AtHKT1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Sodium transporter protein, which plays a central role in plant tolerance to salt. Upon prolongated exposure to high concentrations, Na(+) translocates from the roots to the transpiring leaves where it can increase to toxic level. Involved in Na(+) recirculation from shoots to roots, probably by mediating Na(+) loading into the phloem sap in shoots and unloading in roots, thereby removing large amounts of Na(+) from the shoot. Does not transport K(+) but regulates K(+) nutrient status via its ability to facilitate Na(+) homeostasis. Probably not involved in root uptake of Na(+).
  • 基因功能參考文獻(xiàn)：
    1. The amino-acid substitutionof Asp, replacing Asn in HKT1 altered cation selectivity and uptake dynamics and lead to plant salt tolerance. PMID: 27208305
    2. broad range of metabolites and elements in shoots and roots of different AtHKT1;1 genotypes PMID: 23132143
    3. ABI4 binds two highly related sites within the HKT1;1 promoter.ABI4 downregulates expression of the sodium transporter HKT1;1 in Arabidopsis roots. PMID: 23240817
    4. AtHKT1;1 mediates nernstian sodium channel transport properties in Arabidopsis root stelar cells. PMID: 21931830
    5. Cytokinin acts through ARR1 and ARR12 to regulate AtHKT1;1 expression. PMID: 21105923
    6. Cis-regulatory expression level polymorphisms at the AtHKT1;1 locus, encoding a known sodium (Na(+)) transporter, was identified as being a major factor controlling natural variation in leaf Na(+) accumulation capacity in the A. thaliana population. PMID: 21085628
    7. Overexpress AtHKT1 in the cortex and epidermis upregulated the native AtHKT1. PMID: 20838445
    8. Studies demonstrated that a class of xylem-parenchyma-expressed Na(+)-permeable plant HKT transporters represent a primary mechanism mediating salt tolerance and Na(+) exclusion from leaves. PMID: 19783197
    9. controls Na(+) homeostasis and through this function regulates K(+) nutrient status PMID: 15347798
    10. AtHKT1 selectively unloads sodium directly from xylem vessels to xylem parenchyma cells. PMID: 16359386
    11. SOS3 and AtHKT1 are major determinants of salinity resistance such that sos3 more strongly causes Na+ overaccumulation in roots, whereas athkt1 causes an increase in Na+ levels in the xylem sap and shoots and a concomitant Na+ reduction in roots. PMID: 16540484
    12. Using DNA microarray-based genotyping, identified AtHKT1 as the casual locus driving the natural variation in shoot Na(+) accumulation we observed in Ts-1 and Tsu-1 PMID: 17140289
    13. AtHKT1;1 contributes to the control of both root accumulation of Na(+) and retrieval of Na(+) from the xylem, but is not involved in root influx or recirculation in the phloem. PMID: 17324235
    14. Results demonstrate that tissue-specific regulation of HKT1 is critical for managing Na(+) homeostasis in salt-stressed plants, as well as underscore the breadth and sophistication of plant-microbe interactions PMID: 18624638
    15. Plants constitutively expressing HKT1;1 driven by the cauliflower mosaic virus 35S promoter accumulated high shoot Na+ and grew poorly. PMID: 19584143

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  • 亞細(xì)胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    TrkH potassium transport family, HKT (TC 2.A.38.3) subfamily
  • 組織特異性：
    Highly expressed in roots. Expressed in flowers, leaves and stems. Expressed in the vascular tissues of every organs. In roots, leaves and flower peduncles, it is only expressed in the phloem tissues. Not expressed in root peripheral cells.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ath:AT4G10310

    STRING: 3702.AT4G10310.1

    UniGene: At.2986