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  4. Recombinant Human Disks large homolog 4 (DLG4)

Recombinant Human Disks large homolog 4 (DLG4)

  • 中文名稱：
    人DLG4重組蛋白
  • 貨號：
    CSB-MP006938HU
  • 規(guī)格：
    ￥2376
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
  • Uniprot No.：
  • 別名：
    Postsynaptic density protein 95;PSD-95;Synapse-associated protein 90;SAP-90;SAP90
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length
  • 來源：
    Mammalian cell
  • 分子量：
    109.4 kDa
  • 表達(dá)區(qū)域：
    1-724aa
  • 氨基酸序列
    MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDASDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    C-terminal hFc-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins. Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression.
  • 基因功能參考文獻(xiàn)：
    1. DNA methylation of DLG4 and of human hippocampus and prefrontal cortex in major depression is unchanged in comparison to healthy individuals. PMID: 28645745
    2. results suggest that rare missense mutations in the candidate PSD genes may increase susceptibility to SZ and/or ASD. These findings may strengthen the theory that rare, non-synonymous variants confer substantial genetic risks for these disorders PMID: 27271353
    3. in postsynaptic densities, PSD95 palmitoylation, conformation, and its interactions are dynamic when associated with AMPARs and more stable when associated with NMDARs PMID: 27956638
    4. a dual role for PSD-95 in stabilizing synaptic NMDARs by binding directly to GluN2B but also by promoting synaptic exclusion and degradation of the negative regulator STEP61. PMID: 27457929
    5. the authors integrate molecular and imaging data from animal models and preterm infants, and find that microglial expression of DLG4 plays a role. PMID: 28874660
    6. Phosphorylation at Y397 induced a significant increase in affinity for stargazing. The strategy presented here to generate site-specifically phosphorylated PDZ domains provides a detailed understanding of the role of phosphorylation in the regulation of PSD95 interactions. PMID: 28692247
    7. This study demonstrated that a significant decrease in the protein level of PSD-95 in major depression disorder. PMID: 27661418
    8. These results indicate that PKC promotes synaptogenesis by activating PSD-95 phosphorylation directly through JNK1 and calcium/calmodulin-dependent kinase II and also by inducing expression of PSD-95 and synaptophysin. PMID: 27330081
    9. The differences in cortical NMDAR expression and post-synaptic density protein 95 are present in psychiatric disorders and suicide completion and may contribute to different responses to ketamine. PMID: 26013316
    10. Mutation C>T at the rs13331 in the PSD95 gene is strikingly associated with an increased risk of autism spectrum disorders. PMID: 27072977
    11. Data demonstrate a role for SNAP-25 in controlling PSD-95 clustering and open the possibility that genetic reductions of the protein levels may contribute to the pathology through an effect on postsynaptic function and plasticity. PMID: 25678324
    12. Data indicate the very high affinities of the trimeric ligands to postsynaptic density protein 95 (PSD-95) PDZ domains. PMID: 25658767
    13. In this review, we focus on palmitoylation of PSD-95, which is a major postsynaptic scaffolding protein and makes discrete postsynaptic nanodomains in a palmitoylation-dependent manner and discuss a determinant role of local palmitoylation cycles PMID: 25849917
    14. An association was found between reduced PSD95 in the prefrontal cortex and cognitive impairment in patients with either dementia with Lewy bodies or Parkinson's disease dementia. PMID: 25104558
    15. Docosahexaenoic acid-containing phosphatidylcholines and PSD-95 decrease after loss of synaptophysin and before neuronal loss in patients with Alzheimer's disease. PMID: 25410733
    16. The postsynaptic membrane protein PSD95 was increased in schizophrenia in CA3 tissue, but not in CA1 tissue. PMID: 25585032
    17. The crystal structures of the Dlg4 GK domain in complex with two phosphor-Lgl2 peptides reveal the molecular mechanism underlying the specific and phosphorylation-dependent Dlg/Lgl complex formation. PMID: 24513855
    18. PSD-95 mRNA G-rich region folds into alternate G quadruplex conformations that coexist in equilibrium and miR-125a forms a stable complex with PSD-95 mRNA. PMID: 25406362
    19. Polymorphisms of DRD1, DLG4 and HOMER1 are associated with opiate abuse. PMID: 23044706
    20. The PDZ1 domain of PSD-95 has a shallow binding pocket that accommodates a peptide ligand involving far fewer interactions and a micromolar affinity PMID: 23394112
    21. No association was found between the seven single nucleotide polymorphisms in DLG4 and schizophrenia. PMID: 23921260
    22. A putative role for DLG4 in schizophrenia pathogenesis, evidenced by haplotype association, has been described. PMID: 23936182
    23. interactions of G protein-coupled receptors with postsynaptic density protein 95 PMID: 23691031
    24. Fyn mediates postsynaptic density protein- 95Y523 phosphorylation, which may be responsible for the excitotoxic signal cascades and neuronal apoptosis in brain ischemia and amyloid-beta peptide neurotoxicity. PMID: 22709448
    25. Calcyon forms a novel ternary complex with dopamine D1 receptor through PSD-95 protein and plays a role in dopamine receptor internalization PMID: 22843680
    26. a role of PAR-1 in spine morphogenesis in hippocampal neurons through phosphorylating PSD-95. PMID: 22807451
    27. study adds new components to the multi-dentate membrane targeting mechanism and highlights the role of N- and C-terminal PDZ extensions of PSD-95/ZO-1 in the regulation of syntenin-1 plasma membrane localization PMID: 22673509
    28. Phosphorylation of a PDZ domain extension modulates binding affinity and interdomain interactions in postsynaptic density-95 (PSD-95) protein, a membrane-associated guanylate kinase (MAGUK). PMID: 21965656
    29. tetrad complex shows the close association of the Kir2.1 cytoplasmic domains and the influence of PSD-95 mediated self-assembly on the clustering of these channels PMID: 21756874
    30. In developing visual cortex TrkB and protein kinase M zeta, two critical regulators of synaptic plasticity, facilitate PSD-95 targeting to synapses PMID: 21849550
    31. Genetic and functional analysis of the DLG4 gene encoding the post-synaptic density protein 95 in schizophrenia PMID: 21151988
    32. Our current findings, suggesting decreased levels of PSD95, NR2A, and LRP-1, with elevated levels of caspase-3 and Bcl2 proteins, may reflect or contribute to neuronal and synaptic loss in the amnestic mild cognitive impairment hippocampus PMID: 19774677
    33. PSD-95 has a role in regulating the functional activity and intracellular trafficking of 5-HT2A receptors and possibly other GPCRs PMID: 12682061
    34. A single nucleotide polymorphism was identified as not significantly associated with schizophrenia. PMID: 12950712
    35. PSD-95 and Lin-7b interact with acid-sensing ion channel-3 and have opposite effects on H+- gated current PMID: 15317815
    36. ApoEr2 can form a multiprotein complex with NMDA receptor subunits and PSD95 PMID: 16332682
    37. it is unlikely that the PSD-95 polymorphisms investigated play a substantial role in conferring susceptibility to schizophrenia in the Chinese population PMID: 17093888
    38. GABARAP and DLG4 genes are involved in the etiology of nicotine dependence in European-American smokers. PMID: 17164261
    39. thermodynamic parameters associated with the binding of several series of linear peptides to the third PDZ domain of PSD-9 have been measured using isothermal titration calorimetry PMID: 17474715
    40. The results clearly indicate that D1R-modulated NR1a/NR2B receptor function depends on PSD-95 and is subjected to the regulation of PKA and PKC. PMID: 17506933
    41. PSD-95, the Kv1.3 potassium channel, and insulin receptor serine kinase co-localize to regulate membrane excitability and synaptic transmission at critical locations in the olfactory bulb. PMID: 17854350
    42. PSD-95 levels increased postnatally to reach a stable plateau by early childhood with a slight reduction in late adolescence and early adulthood PMID: 17916412
    43. These results suggest that NR2A and NR2B may associate with PSD-95 but with different affinities. This may be important in the determination of the lateral mobility of NMDA receptor subtypes in post-synaptic membranes. PMID: 18308477
    44. Increase in postsynaptic density protein PSD-95 expression positively correlates with beta amyloid and phosphorylated Tau proteins in Alzheimer's disease cases. PMID: 18424056
    45. This study found that the PSD-95 protein level significationalty elevated in pateint with deression. PMID: 18570704
    46. The DLG4 protein enhances the resensitization of the D1 DA receptor by accelerating D1 receptor recycling to the cell membrane. PMID: 19274064
    47. These data suggest that NMDA receptor complex formation, localization, and downstream signaling may be abnormal in schizophrenia as PSD95, SynGAP and MUPP1 expression is altered. PMID: 19483657
    48. An altered association between membrane-associated guanylate kinases (such as PSD-95) and NMDA receptors in mutant huntingtin-expressing cells contributes to increased susceptibility to excitotoxicity. PMID: 19726651
    49. validated occurrence of an unusual TG 3' splice site in intron 5 PMID: 17672918

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  • 亞細(xì)胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side. Cell junction, synapse, postsynaptic density. Cell junction, synapse. Cytoplasm. Cell projection, axon. Cell projection, dendritic spine. Cell projection, dendrite. Cell junction, synapse, presynapse.
  • 蛋白家族：
    MAGUK family
  • 組織特異性：
    Brain.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2903

    OMIM: 602887

    KEGG: hsa:1742

    STRING: 9606.ENSP00000293813

    UniGene: Hs.463928