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  4. Recombinant Human Four and a half LIM domains protein 2 (FHL2)

Recombinant Human Four and a half LIM domains protein 2 (FHL2)

  • 中文名稱：
    人FHL2重組蛋白
  • 貨號：
    CSB-YP617906HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人FHL2重組蛋白
  • 貨號：
    CSB-EP617906HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人FHL2重組蛋白
  • 貨號：
    CSB-EP617906HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人FHL2重組蛋白
  • 貨號：
    CSB-BP617906HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人FHL2重組蛋白
  • 貨號：
    CSB-MP617906HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    AAG 11; AAG11; Aging associated gene 11; Down regulated in rhabdomyosarcoma LIM protein; Downregulated in rhabdomyosarcoma LIM protein; DRAL; FHL 2; FHL-2; Fhl2; FHL2 protein; FHL2_HUMAN; Four and a half LIM domain protein 2; Four and a half LIM domains 2; Four and a half LIM domains protein 2; KIAA0990; LIM domain protein DRAL; Skeletal muscle LIM protein 3; Skeletal muscle LIM-protein 3; SLIM 3; SLIM-3; SLIM3
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-279
  • 氨基酸序列
    MTERFDCHHC NESLFGKKYI LREESPYCVV CFETLFANTC EECGKPIGCD CKDLSYKDRH WHEACFHCSQ CRNSLVDKPF AAKEDQLLCT DCYSNEYSSK CQECKKTIMP GTRKMEYKGS SWHETCFICH RCQQPIGTKS FIPKDNQNFC VPCYEKQHAM QCVQCKKPIT TGGVTYREQP WHKECFVCTA CRKQLSGQRF TARDDFAYCL NCFCDLYAKK CAGCTNPISG LGGTKYISFE ERQWHNDCFN CKKCSLSLVG RGFLTERDDI LCPDCGKDI
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes.
  • 基因功能參考文獻：
    1. FHL2 is an important prognostic factor in cervical cancer (CC) and that it plays a crucial oncoprotein role by promoting cell proliferation and inhibiting apoptosis in CC. PMID: 29800735
    2. FHL2 and iASPP interacted with each other and co-localized in both nucleus and cytoplasm. Either FHL2 or iASPP silenced could reduce cell proliferation, induce cell cycle arrest at G0/G1 phase, and increase cell apoptosis. PMID: 28402264
    3. FHL2 phosphorylation by FAK is a critical, mechanically dependent step in signaling from soft matrices to the nucleus to inhibit cell proliferation by increasing p21 expression. PMID: 27742790
    4. FHL2 facilitates ovarian granulosa cell tumor progression via controlling AKT1 transcription. PMID: 27415427
    5. we determined the molecular mechanism responsible for IER3 degradation, involving a ternary complex of IER3, MDM2 and FHL2, which may contribute to cervical tumor growth. Furthermore, we demonstrated that FHL2 serves as a scaffold for E3 ligase and its substrate during the ubiquitination reaction, a function that has not been previously reported for this protein PMID: 26973248
    6. FHL2 interacts with ADAM-17 in normal, dysplastic and malignant colon epithelial cells. Colocalisation of these proteins is more frequent in malignant than in normal and dysplastic cells, suggesting a role for ADAM-17/FHL2 complex in the development of colorectal cancer. PMID: 28349819
    7. FHL2 overexpression could enhance the differentiation and mineralization of human dental pulp cells PMID: 25662359
    8. findings show that mAbp1 and FHL2 are novel binding partners that differentially regulate Rho GTPase signaling and MTLn3 breast cancer cell invasion PMID: 27129278
    9. These results identify FHL2 as a novel gene associated with asthma severity in human. PMID: 26222912
    10. FHL2 might interact with Runx2 to mediate mesenchymal cell differentiation at the early stages of tooth development and human dental pulp cell differentiation. PMID: 26759258
    11. FHL2 overexpression could contribute to the growth, proliferation, invasiveness, and metastasis of human tongue squamous cell carcinoma. PMID: 26759260
    12. KLF8-induced FHL2 activation is a novel and critical signaling mechanism underlying human colorectal cancer invasion and metastasis. PMID: 26320172
    13. Studies indicate that the LIM-only protein FHL2 interactome is functionally involved in the cardiovascular system. PMID: 26548523
    14. we conclude that FHL2 has both structural and functional protein-protein interactions with b-catenin in the podocyte nucleus and that FHL2 protein inhibition can mitigate Wnt/b-catenin-induced podocytopathy. PMID: 25855776
    15. Describe the molecular network governing FHL2 expression, and FHL2-linked cancers and the underlying molecular machinery. Review. PMID: 26211626
    16. Tumoral expression of nuclear cofactor FHL2 is associated with lymphatic metastasis in sporadic but not in hereditary nonpolyposis colorectal cancer-associated colorectal cancer. PMID: 25554651
    17. results provide evidence for the importance of the focal adhesion protein FHL2 in pancreatic cancer cell survival, proliferation and radiosensitivity PMID: 25917075
    18. FHL2 is down-regulated in HCM; both FHL2 wild type and variants partially protected phenylephrine- or endothelin-1-induced hypertrophy in cardiac myocytes PMID: 25358972
    19. FHL2 downregulation has a role in the pathogenesis of myeloid malignancies. PMID: 25179730
    20. HPV16 E6 oncoprotein interacted and impaired the subcellular distribution of FHL2. PMID: 25540819
    21. tumoural TGF-beta1 secretion seems to induce nuclear translocation of co-factor FHL2 mediating progressive keratin expression in pilomatricoma. PMID: 25477051
    22. Enhanced FHL2 and TGF-beta1 expression is correlated with poor survival in human malignant melanoma PMID: 25596251
    23. FHL2 is a transcriptional coactivator of LXRs and may be an important determinant of cholesterol metabolism in SMCs PMID: 25332231
    24. High FHL2 expression is associated with colon cancer. PMID: 24008552
    25. Data suggest Fhl1 (four-and-a-half LIM domains protein 1) and FHL2 (four-and-a-half LIM domains protein 2) bind to and regulate activity of Prkd1 (protein kinase D 1) in cardiac/ventricular myocytes; knockdown of FHL2 down-regulates Prkd1 activation. PMID: 24219103
    26. The calpain-cleaved filamin fragment and FHL2 are present in the nucleus only in CRPC. PMID: 23801747
    27. higher FHL2 expression in malignant epithelial cells correlates with progressive disease in patients with colorectal cancer, suggesting that FHL2 is a prognostic indicator for the development of metachronous metastases and for overall survival PMID: 23756870
    28. FHL2 acts as an oncogene in osteosarcoma cells and contributes to tumorigenesis through Wnt signaling PMID: 23383046
    29. PCBP2 is an RNA-binding protein that modulates glioma growth by regulating FHL3 PMID: 23585479
    30. The four and a half LIM-only protein 2 regulates liver homeostasis and contributes to carcinogenesis. PMID: 22796152
    31. Deletion of FHL2 protein by FHL2 small interfering RNA impaired VEGF production under hypoxia conditions, and also suppressed endothelial progenitor cell angiogenic functions. PMID: 23413425
    32. FHL2 increases the stability of the TGF-beta pathway positive regulator Arkadia by inhibiting its ubiquitination and cooperates with Arkadia to activate TGF-beta signaling. PMID: 23212909
    33. Overexpression of Id3 markedly promoted the proliferation and invasive capacity of MCF-7 cells; however, these effects were significantly suppressed by the overexpression of FHL2. PMID: 22882857
    34. FHL2 modulates calcineurin-dependent activation of NFAT target genes. PMID: 22851699
    35. On influenza A virus infection FHL2 translocates into the nucleus, potentiating the IRF-3-dependent transcription of the IFNbeta gene. PMID: 22417706
    36. FHL2 directly interacts with HIF-1alpha to repress transcriptional activity. PMID: 22219185
    37. association of FHL2 with Nur77 plays a pivotal role in vascular disease. PMID: 22049082
    38. Overexpression of FHL2 in peritumoural myofibroblasts correlated to lymphatic metastasis in sporadic colon cancer but not in hereditary non-polyposis colorectal cancer. PMID: 21826055
    39. Our data suggest a role of FHL2 in odontoblast differentiation and dentin formation both in normal and in carious teeth PMID: 21308406
    40. In human colorectal carcinoma but not in low-grade dysplasia, we detected up-regulation and enhanced nuclear localization of FHL2, indicating the activation of FHL2 during the development of malignancy PMID: 20442768
    41. our results indicate FHL2 could exert anti-apoptotic effect independent of tumor growth suppression PMID: 21377781
    42. cooperative regulation of estrogen signaling by FHL2 and Smad4 in breast cancer cells, and might provide a new regulation mechanism underlying breast cancer development and progression PMID: 20734429
    43. In the absence of transgenic FHL2, CCL19-induced bone marrow-derived dendritic cell migratory speed, persistence, and directionality were markedly increased in vitro and in vivo. PMID: 20592280
    44. Sp1 up-regulates FHL2 expression in gastrointestinal cancers through transcription regulation. PMID: 20607723
    45. FHL2 is a potent epithelial-mesenchymal transition (EMT) inducer and might be an important mediator for invasion and/or metastasis of colon cancer. PMID: 20460358
    46. Aryl hydrocarbon receptor (AhR) modulation of androgen receptor activity is differentially altered by the level of FHL2 and AhR present in the cell. PMID: 19815066
    47. Insulin-like growth factor-binding protein 5 (IGFBP-5) interacts with a four and a half LIM protein 2 (FHL2). PMID: 11821401
    48. TUCAN/CARDINAL and DRAL participate in a common pathway for modulation of NF-kappaB activation. PMID: 12067710
    49. evidence of a functional interaction between the promyelocytic leukemia zinc finger protein (PLZF) and DRAL/FHL2 PMID: 12145280
    50. FHL2 might enforce beta-catenin transactivation activity in cancer cells PMID: 12466281

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  • 亞細胞定位：
    Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, Z line.
  • 組織特異性：
    Expressed in skeletal muscle and heart.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 3703

    OMIM: 602633

    KEGG: hsa:2274

    STRING: 9606.ENSP00000322909

    UniGene: Hs.443687