Recombinant Human Ubiquitin carboxyl-terminal hydrolase 1 (USP1)
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中文名稱:人USP1重組蛋白
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貨號(hào):CSB-EP025695HUa0
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規(guī)格:¥1344
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圖片:
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其他:
產(chǎn)品詳情
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純度:Greater than 85% as determined by SDS-PAGE.
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生物活性:Not Test
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基因名:
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Uniprot No.:
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別名:(Deubiquitinating enzyme 1)(hUBP)(Ubiquitin thioesterase 1)(Ubiquitin-specific-processing protease 1)
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種屬:Homo sapiens (Human)
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蛋白長(zhǎng)度:Full Length
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來源:E.coli
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分子量:94.3
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表達(dá)區(qū)域:1-785aa
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氨基酸序列MPGVIPSESNGLSRGSPSKKNRLSLKFFQKKETKRALDFTDSQENEEKASEYRASEIDQVVPAAQSSPINCEKRENLLPFVGLNNLGNTCYLNSILQVLYFCPGFKSGVKHLFNIISRKKEALKDEANQKDKGNCKEDSLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGYLQHDAQEVLQCILGNIQETCQLLKKEEVKNVAELPTKVEEIPHPKEEMNGINSIEMDSMRHSEDFKEKLPKGNGKRKSDTEFGNMKKKVKLSKEHQSLEENQRQTRSKRKATSDTLESPPKIIPKYISENESPRPSQKKSRVKINWLKSATKQPSILSKFCSLGKITTNQGVKGQSKENECDPEEDLGKCESDNTTNGCGLESPGNTVTPVNVNEVKPINKGEEQIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDKGNFVVDQMCEIGKPEPLNEEEARGVVENYNDEEVSIRVGGNTQPSKVLNKKNVEAIGLLGGQKSKADYELYNKASNPDKVASTAFAENRNSETSDTTGTHESDRNKESSDQTGINISGFENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. -
蛋白標(biāo)簽:N-terminal 6xHis-tagged
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產(chǎn)品提供形式:Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
緩沖液:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
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復(fù)溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲(chǔ)存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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注意事項(xiàng):Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
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Datasheet & COA:Please contact us to get it.
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.
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基因功能參考文獻(xiàn):
- USP1 and capital ER, Cyrilliccapital EN, Cyrillic domain of Bcr-Abl oncoprotein colocalize in a model chronic myeloid leukemia cell system. PMID: 30480413
- Our preclinical studies provide the framework for clinical evaluation of USP1 inhibitors, alone or in combination, as a potential novel multiple myeloma therapy. PMID: 28270494
- There is evidence that USP1/WDR48 complex promotes cancer stem cell conservation and regulation of DNA damage repair. [REVIEW] PMID: 28302046
- These results outline a novel mechanism for the control of TBK1 activity and suggest USP1-UAF1 complex as a potential target for the prevention of viral diseases. PMID: 29138248
- USP1 is a key senescence regulator controlling genomic integrity. PMID: 27160904
- High USP1 expression is associated with glioma. PMID: 26951930
- USP1-UAF1 complex promotes homologous recombination repair via multiple mechanisms: through FANCD2 deubiquitination, as well as by interacting with RAD51AP1. PMID: 27463890
- The results suggest that silencing USP1 inhibits cell proliferation and invasion in U2OS cells. Therefore, USP1 may provide a novel therapeutic target for the treatment of osteosarcoma. PMID: 27840911
- Translational regulation of the mRNA encoding USP1 is involved in the DNA damage response as a determinant of cisplatin resistance. PMID: 26825230
- Ubiquitination-specific proteases 1 (USP1)-mediated protein stabilization promotes glioblastoma (GBM) stem-like cells maintenance and treatment resistance, thereby providing a rationale for USP1 inhibition as a potential therapeutic approach against GBM. PMID: 26032834
- analysis of the function and regulation of the USP1-UAF1 complex PMID: 26758085
- USP1, p21 and Spartan are translesion DNA synthesis regulators. (Review) PMID: 26002196
- No correlation was observed between the protein level of ubiquitin-specific protease 1 (USP1) and ubiquitinated PCNA in BAF180 expressing cells PMID: 26117423
- USP1 phosphorylation at S313 is not critical for PCNA deubiquitination, neither for binding to UAF1 in a cellular environment. PMID: 25744535
- Coimmunoprecipitation experiments indicated that human papillomavirus type 31 E1 assembles into a ternary complex with UAF1 and any one of these three USPs: USP1, USP12 and USP46. PMID: 24850727
- Our findings revealed an intriguing mechanism of regulating USP1 activity that combines phosphorylation of a key serine residue in USP1 and the specific interaction of USP1 with a WD40-repeat protein UAF1 PMID: 23116119
- USP1 and UAF1 form a complex in the cytoplasm that subsequently translocates to the nucleus through import mediated by USP1 nuclear localization signals. PMID: 22701671
- data identified a novel USP1-PHLPP1-Akt signaling axis, and decreased USP1 level in lung cancer cells may play an important role in lung cancer progress. PMID: 22426999
- Studies indicate that USP1 and ELG1 as regulators of PCNA ubiquitylation. PMID: 21640107
- Study shows that the deubiquitinating enzyme USP1 promotes ID protein stability and stem cell-like characteristics in osteosarcoma. PMID: 21925315
- USP1 levels were kept low in G1 to provide a permissive condition for inducing proliferating cell nuclear antigen (PCNA) monoubiquitination in response to ultraviolet (UV) damage before DNA replication. PMID: 21768287
- USP1-regulated and damage-specific DNA-binding protein 1 (DDB1)-dependent mechanisms that are involved in the downregulation of activated CHK1 in human cells. PMID: 21389083
- USP1 deubiquitinates FANCD2 protein when cells exit S phase or recommence cycling after a DNA damage insult and may play a critical role in the Fanconi anemia pathway by recycling FANCD2. PMID: 15694335
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亞細(xì)胞定位:Nucleus.
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蛋白家族:Peptidase C19 family
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