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  4. Recombinant Mouse Piwi-like protein 2 (Piwil2), partial

Recombinant Mouse Piwi-like protein 2 (Piwil2), partial

  • 中文名稱：
    小鼠Piwil2重組蛋白
  • 貨號：
    CSB-YP806517MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Piwil2重組蛋白
  • 貨號：
    CSB-EP806517MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Piwil2重組蛋白
  • 貨號：
    CSB-EP806517MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Piwil2重組蛋白
  • 貨號：
    CSB-BP806517MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Piwil2重組蛋白
  • 貨號：
    CSB-MP806517MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Piwil2
  • Uniprot No.：
  • 別名：
    Piwil2; Mili; Piwi-like protein 2; EC 3.1.26.-
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. During piRNA biosynthesis, plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-competent' piRNA endoribonuclease that cleaves primary piRNAs, which are then loaded onto 'slicer-incompetent' PIWIL4. PIWIL2 slicing produces a pre-miRNA intermediate, which is then processed in mature piRNAs, and as well as a 16 nucleotide by-product that is degraded. Required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulates expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Represses circadian rhythms by promoting the stability and activity of core clock components ARNTL/BMAL1 and CLOCK by inhibiting GSK3B-mediated phosphorylation and ubiquitination-dependent degradation of these proteins.
  • 基因功能參考文獻(xiàn)：
    1. When MILI (or PIWIL2), a PIWI family member, was depleted by gene knockout, almost all PIWI-interacting RNA disappeared. PMID: 28115634
    2. MILI is responsible for DNA methylation of a larger subset of TE families than MIWI2. PMID: 26279574
    3. piRNA biogenesis triggered by PIWI slicing, and promoted by EXD1, ensures that the same guides instruct PIWI proteins in the nucleus and cytoplasm. PMID: 26669262
    4. Knockdown of MILI in B16 cell line could activate MAGEA expression and increase the cell migration ability, whereas the overexpression of MILI in B16BL6 cell line could inhibit MAGEA expression and decrease the cell migration ability. PMID: 25582775
    5. Report shows that fly Aub, Bombyx mori Siwi, and mouse MILI select targets bearing a t1A nucleotide irrespective of the identity of the g1 nucleotide of their piRNA guide. PMID: 25453759
    6. Mice with a global deletion of all three piwi genes, Miwi, Mili, and Miwi2, are able to maintain long-term hematopoiesis with no observable effect on the homeostatic hematopoietic stem cells compartment in adult mice. PMID: 24058407
    7. The in vitro studies show estrogen can downregulate the expression of miwi and mili. PMID: 22628161
    8. characterize the genome-wide target RNA repertoire of Mili (Piwil2) and Miwi (Piwil1), two Piwi proteins expressed in postnatal testis PMID: 22842725
    9. a new role for Piwil2 in DNA repair PMID: 22110608
    10. cycles of intra-Mili secondary piRNA biogenesis fuel piRNA amplification that is absolutely required for LINE1 silencing PMID: 22020280
    11. The results suggest broader roles for Piwil2 in genome surveillance beyond the germ line and a possible role in regulating the cell cycle of mesenchymal stem cells. PMID: 20460113
    12. Data suggest that the formation of complexes between MILI, MIWI and TDRD1/MTR-1 is critical for the integrated subcellular localizations of these proteins, and is presumably essential for spermatogenesis. PMID: 19735482
    13. The Piwil2 protein modulates expression of stem cell specific genes, including Pdgfrb, Slc2a1, Gja7, CD90, Itga6, CD9, Hsp90a, and Stra8. PMID: 16261612
    14. description of a new class of small RNAs that bind to MILI in mouse male germ cells, where they accumulate at the onset of meiosis PMID: 16751777
    15. Our observations suggest a conserved function for Piwi-clade proteins in the control of transposons in the germline. PMID: 17395546
    16. evidence of an adaptive amplification loop in which MILI catalyzes formation of piRNA 5'ends; Mili mutants derepress LINE-1 (L1) & intracisternal A particle & lose DNA methylation of L1 elements, showing conserved role for PIWI in transposon suppression PMID: 17446352
    17. Data strongly suggest that MILI and MIWI2 play essential roles in establishing de novo DNA methylation of retrotransposons in fetal male germ cells. PMID: 18381894
    18. MILI may positively regulate translation and that such regulation is required for germ line stem cell self-renewal. PMID: 19114715
    19. Results suggest that Mili interacts with Tdrd1 in the nuage and chromatoid body, and that this interaction does not contribute to piRNA biogenesis but represents a regulatory mechanism that is critical for spermatogenesis. PMID: 19345100
    20. isolated Mili-containing complexes from adult mouse testes and identified Tudor domain-containing protein-1 (Tdrd1) as a factor specifically associated with the Mili piRNP throughout spermatogenesis PMID: 19465913
    21. purified the three murine Piwi family proteins, MILI, MIWI, and MIWI2, from mouse germ cells and characterized their interacting protein partners PMID: 19584108

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Argonaute family, Piwi subfamily
  • 組織特異性：
    Expressed in adult testis, specifically in spermatocytes and in spermatogonia. Only detected in primordial germ cells of both sexes. Widely expressed in tumors. Also present at early stages of oocyte growth. Present in the mitotic spermatogonia. Not detec
  • 數(shù)據(jù)庫鏈接：