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  4. Recombinant Mouse UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (Ogt), partial

Recombinant Mouse UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (Ogt), partial

  • 中文名稱：
    小鼠Ogt重組蛋白
  • 貨號：
    CSB-YP806073MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Ogt重組蛋白
  • 貨號：
    CSB-EP806073MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Ogt重組蛋白
  • 貨號：
    CSB-EP806073MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Ogt重組蛋白
  • 貨號：
    CSB-BP806073MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Ogt重組蛋白
  • 貨號：
    CSB-MP806073MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Ogt
  • Uniprot No.：
  • 別名：
    Ogt; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; EC 2.4.1.255; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit; OGT
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Probably by glycosylating KMT2E/MLL5, stabilizes KMT2E/MLL5 by preventing its ubiquitination.Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O-GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues. O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex. Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2. O-glycosylates HCFC1 and regulates its proteolytic processing and transcriptional activity. Regulates mitochondrial motility in neurons by mediating glycosylation of TRAK1. Glycosylates HOXA1. O-glycosylates FNIP1.
  • 基因功能參考文獻(xiàn)：
    1. Ogt is not only important for triggering B cell receptor-mediated signaling pathways, but also for the survival of mature, germinal center B cells. PMID: 29187734
    2. Data show that postsynaptic deletion of O-GlcNAc transferase (OGT) leads to fewer morphological synapses. PMID: 28143929
    3. miR-24-1 may regulate mouse hepatocarcinoma cells migration and invasion, at least partially through targeting OGT. PMID: 28499244
    4. O-GlcNAc modification is essential for cold-induced thermogenesis and mitochondrial biogenesis in brown adipose tissue. PMID: 28637651
    5. This work uncovers that URI-regulated OGT confers c-MYC-dependent survival functions in response to glucose fluctuations. PMID: 27505673
    6. This study identifies OGT activity as an important regulator of SC functions such as myelin maintenance and axonal support. PMID: 27629714
    7. cullin 3 (CUL3), a cullin family E3 ubiquitin ligase, down-regulates the expression of the O-GlcNAc transferase (OGT) and inhibits STAT3 O-GlcNAcylation. PMID: 28280036
    8. OGT functions in metastatic spread of HPV E6/E7-positive tumor cells to the lungs through E6/E7, HCF-1 and CXCR4 PMID: 27845045
    9. Beyond its well-known role in adding beta-O-GlcNAc to serine and threonine residues of nuclear and cytoplasmic proteins, OGT also acts as a protease in the maturation of the cell cycle regulator, HCF-1, and serves as an integral member of several protein complexes, many of them linked to gene expression. (Review) PMID: 27294441
    10. OGT overexpression increased the level of OGA, suggesting a compensatory mechanism for the aberrant O-GlcNAcylation. PMID: 26286378
    11. O-GlcNAc transferase is at least partially required for maintaining cellular proliferative and migratory capacities of cardiomyocytes PMID: 26565625
    12. The hyperphagia derived from the paraventricular nucleus of the hypothalamus, where loss of OGT was associated with impaired satiety. PMID: 26989246
    13. 26S proteasome-mediated OGT reduction contributed to hypoxia-induced vascular endothelial inflammatory response. PMID: 24788415
    14. Glucosamine-induced OGT activation mediates glucose production through cleaved Notch1 and FoxO1, which contributes to the regulation of maintenance of self-renewal in mouse embryonic stem cells. PMID: 24730386
    15. Study shows that OGT expression is enriched in hypothalamic AgRP neurons and induced by fasting and ghrelin. Pharmacogenetical activation of AgRP neurons suppresses the thermogenic program in white fat (WAT), while the selective knockout of Ogt in AgRP neurons inhibits neuronal activity, promotes WAT browning, and protects mice against diet-induced obesity. PMID: 25303527
    16. OGT serves as a key placental biomarker and functions as an important mediator of the maternal changes occurring in response to stress. PMID: 24979775
    17. cardiomyocyte-specific OGT KO mice had dilated hearts, signs of heart failure and low viability. PMID: 24186210
    18. Treatment with GlcN, in contrast, inhibits LPS-ind inflammation and decreased LPS-mediated recruitment of OGT, mSin3A, and HDACs. PMID: 23824843
    19. Tet1 could also interact with the O-GlcNAc transferase (Ogt) and be O-GlcNAcylated. PMID: 23729667
    20. Exercise-induced physiological hypertrophy is associated with a decrease in protein O-GlcNAcylation and expression of O-GlcNAc transferase in the heart. PMID: 21493720
    21. These studies identified OGT as a promising placental biomarker of maternal stress exposure that may relate to sex-biased outcomes in neurodevelopment. PMID: 23487789
    22. Tet1 and Tet2 are stable partners of Ogt in the nucleus of embryonic stem cells. PMID: 23352454
    23. O-GlcNAc glycosylation in mice has chondromodulating activity PMID: 22859309
    24. Hsp90 is involved in the regulation of OGT and O-GlcNAc modification and that Hsp90 inhibitors might be used to modulate O-GlcNAc modification and reverse its adverse effects in human diseases. PMID: 22496241
    25. Data show that Polycomb repressive complex 2 is necessary to maintain normal levels of OGT and for the correct cellular distribution of O-GlcNAc. PMID: 21606357
    26. denitrosylation of S-nitrosylated OGT is a direct mechanism for upregulation of OGT activity by which immune defense is critically controlled in LPS-stimulated innate immune response. PMID: 21453677
    27. Study shows that ChREBP interacts with OGT and is subjected to O-GlcNAcylation in liver cells PMID: 21471514
    28. This transferase mediates a novel hexosamine-dependent signal transduction pathway. It is now sequenced and compared to the human and rat ogt locus. PMID: 12504895
    29. Ogt mutation results in the loss of O-GlcNAc and causes T-cell apoptosis, neuronal tau hyperphosphorylation, and fibroblast growth arrest with altered expression of c-Fos, c-Jun, c-Myc, Sp1, and p27 PMID: 14749383
    30. Overall, transcriptional inhibition is related to the integrated effect of O-GlcNAc by direct modification of critical elements of the transcriptome and indirectly through O-GlcNAc modification of the proteasome. PMID: 16966374
    31. CaMKIV activates OGT (o-linked GlcNAc transferase), and OGT has an essential role on the process of CaMKIV-dependent AP-1 activation under depolarization in neuronal cells. PMID: 18029144
    32. insulin activation of OGT results in increased O-GlcNAc modification of OGT and other proteins including, signal transducer and activator of transcription 3 PMID: 18519567
    33. Exerts pro-survival actions during hypoxia-reoxygenation in cardiac myocytes, particularly at the level of mitochondria. PMID: 18539296
    34. these data suggest that O-GlcNAc regulates Akt signaling in hepatic models under euglycemic conditions PMID: 18728220
    35. MYPT1 and CARM1 are substrates of OGT and function to alter OGT substrate specificity in vitro PMID: 18840611

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus. Cell membrane. Mitochondrion membrane. Cell projection.
  • 蛋白家族：
    Glycosyltransferase 41 family, O-GlcNAc transferase subfamily
  • 數(shù)據(jù)庫鏈接：