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  4. Recombinant Pseudomonas phage phi6 RNA-directed RNA polymerase (P2), partial

Recombinant Pseudomonas phage phi6 RNA-directed RNA polymerase (P2), partial

In Stock
  • 中文名稱：
  • 貨號：
    CSB-EP319967PUV
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    P2
  • Uniprot No.：
  • 別名：
    Protein P2
  • 種屬：
    Pseudomonas phage phi6 (Bacteriophage phi-6)
  • 蛋白長度：
    Partial
  • 來源：
    E.coli
  • 分子量：
    27.2 kDa
  • 表達(dá)區(qū)域：
    310-485aa
  • 氨基酸序列
    NKEEKVKEWSLCVATDVSDHDTFWPGWLRDLICDELLNMGYAPWWVKLFETSLKLPVYVGAPAPEQGHTLLGDPSNPDLEVGLSSGQGATDLMGTLLMSITYLVMQLDHTAPHLNSRIKDMPSACRFLDSYWQGHEEIRQISKSDDAILGWTKGRALVGGHRLFEMLKEGKVNPSP
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Rna-dependent RNA polymerase part of the packaging complex that packages the viral RNA segments, replicate them into a double-stranded form and transcribe them.
  • 基因功能參考文獻(xiàn)：
    1. Purified polymerase P2 is able to transcribe dsRNA, but transcription behavior of bacteriophage Phi6 RNA segment L by both wild-type and mutant polymerases is different from that seen in capsid structures. PMID: 23864621
    2. The structurally flexible hinge region appeared to be involved in the control of priming platform movement. PMID: 22770923
    3. Divalent cations modulate the structural dynamics of phi6 RNA-dependent RNA polymerase. PMID: 22205747
  • 亞細(xì)胞定位：
    Virion. Note=Found in the capsid (12 copies). Prior to RNA packaging, localizes on the inner procapsid surface near the three-fold axis of symmetry.
  • 數(shù)據(jù)庫鏈接：

    KEGG: vg:956436