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  4. Recombinant Saccharomyces cerevisiae ATP-dependent DNA helicase SRS2 (SRS2), partial

Recombinant Saccharomyces cerevisiae ATP-dependent DNA helicase SRS2 (SRS2), partial

  • 中文名稱：
    釀酒酵母SRS2重組蛋白
  • 貨號：
    CSB-YP321041SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母SRS2重組蛋白
  • 貨號：
    CSB-EP321041SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母SRS2重組蛋白
  • 貨號：
    CSB-EP321041SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母SRS2重組蛋白
  • 貨號：
    CSB-BP321041SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母SRS2重組蛋白
  • 貨號：
    CSB-MP321041SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SRS2
  • Uniprot No.：
  • 別名：
    SRS2; HPR5; RADH; YJL092W; J0913ATP-dependent DNA helicase SRS2; EC 3.6.4.12
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    ATP-dependent DNA helicase involved in DNA repair at least for UV-induced lesions. The polarity of the helicase activity was determined to be 3' to 5'.
  • 基因功能參考文獻：
    1. Two main activities of Srs2, strippase and helicase, counteract toxic joint intermediates formed during break-induced replication by preventing their formation and promoting their disruption, respectively. PMID: 29176630
    2. Srs2 rapidly disrupts small heteroduplex DNA joints bound to Rad51 filaments. PMID: 29241544
    3. These data establish a biochemical mechanism for the role of Srs2 in crossover suppression by promoting synthesis-dependent strand annealing through disruption of DNA polymerase delta-extended D-loops. PMID: 28535142
    4. Uls1 contributes to the pathway where intermediates generated at replication forks are dismantled by Srs2 bound to SUMO-PCNA. Upon ULS1 deletion, accumulating Srs2-SUMO-unable to bind PCNA-takes part in an alternative PCNA-independent recombination repair salvage pathway(s) PMID: 28341648
    5. Srs2 unfolds trinucleotide repeat hairpin repetitively, and its activity depends on the folding strength and the total length of TNR hairpin. PMID: 26004439
    6. When Rad51 is bound on the double-stranded DNA, its interaction with Srs2 blocks the helicase (DNA unwinding) activity of Srs2. PMID: 24576606
    7. Srs2 exhibits ATP-dependent repetitive motion on single-stranded DNA and this activity prevents re-formation of the Rad51 filament. PMID: 23939144
    8. Rad52 mutant cannot restore viability of srs2Delta cells that accumulate intertwined recombination intermediates which are normally processed by Srs2 post-synaptic functions PMID: 24130504
    9. Srs2 disrupts Rad51-containing complexes from chromosomes during meiosis. PMID: 23770697
    10. Putative antirecombinase Srs2 DNA helicase promotes noncrossover homologous recombination avoiding loss of heterozygosity. PMID: 24043837
    11. The hDNA data are consistent with the proposed role of Sgs1 in the dissolution of double HJs, and we propose that Srs2 dismantles nicked HJs. PMID: 23516370
    12. Srs2 unwinding is inhibited by ssDNA binding protein RPA in vitro PMID: 22921573
    13. The SUMO-interacting motif region is required for Srs2 sumoylation, likely by binding to SUMO-charged Ubc9. PMID: 22705796
    14. suppression of homologous recombination in postreplication repair-deficient cells by Srs2 and PCNA sumoylation is required for checkpoint activation and checkpoint maintenance during UV irradiation PMID: 20713444
    15. the importance of the ATP hydrolysisfueled DNA motor activity in SRS2 functions PMID: 15047689
    16. suppression of homeologous recombination not relaxed in the srs2 mutant PMID: 15611162
    17. Srs2 assoiates with Mre11 in different subcomplexes following checkpoint activation and CDK1-mediated Srs2 phosphorylation. PMID: 15964827
    18. These findings suggest that the hFBH1 helicase is a functional human orthologue of budding yeast Srs2 that also possesses self-regulation properties necessary to execute its recombination functions. PMID: 17724085
    19. The Srs2 helicase activity is stimulated by Rad51 filaments on dsDNA PMID: 18243118
    20. Srs2 maintains its facile unwinding of triplet repeat hairpins embedded within duplex DNA, supporting the genetic evidence that Srs2 is a key helicase in Saccharomyces cerevisiae for preventing expansions. PMID: 18440969
    21. Description of two new srs2 mutants that have acquired the ability to perform recombinational repair in a rad18 context, but have retained the ability to reverse toxic structures in a RAD18 context. PMID: 18658248
    22. Results suggest that Srs2 promotes fork reversal in repetitive sequences, preventing repeat instability and fragility; in the absence of Srs2 or Sgs1, DNA damage accumulates and is processed by homologous recombination, triggering repeat rearrangements. PMID: 19136956
    23. Results characterize Srs2 function in vivo and describe a novel mechanism for regulating the initiation of homologous recombination. PMID: 19506039
    24. Data show that a physical interaction between Rad51 and the C-terminal region of Srs2 triggers ATP hydrolysis within the Rad51 filament, causing Rad51 to dissociate from DNA. PMID: 19595720
    25. functional significance of the Rad51-Rad52 and Rad51-Srs2 complexes in the control of presynaptic filament assembly and disassembly. PMID: 19605344
    26. Translocations rely on homologous recombination (HR) proteins, such as DNA repair enzymes, as well as on the break-induced replication-specific protein Pol32 and on Srs2 PMID: 19651902

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Helicase family, UvrD subfamily
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YJL092W

    STRING: 4932.YJL092W