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  4. Recombinant Saccharomyces cerevisiae Vacuolar protein sorting-associated protein 33 (VPS33), partial

Recombinant Saccharomyces cerevisiae Vacuolar protein sorting-associated protein 33 (VPS33), partial

  • 中文名稱：
    釀酒酵母VPS33重組蛋白
  • 貨號(hào)：
    CSB-YP322690SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母VPS33重組蛋白
  • 貨號(hào)：
    CSB-EP322690SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母VPS33重組蛋白
  • 貨號(hào)：
    CSB-EP322690SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母VPS33重組蛋白
  • 貨號(hào)：
    CSB-BP322690SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母VPS33重組蛋白
  • 貨號(hào)：
    CSB-MP322690SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    VPS33
  • Uniprot No.：
  • 別名：
    VPS33; SLP1; VAM5; YLR396C; L8084.15Vacuolar protein sorting-associated protein 33; Protein SLP1; Vacuolar morphogenesis protein 5
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Essential for vacuolar biogenesis, maturation and function. Involved in the sorting of vacuolar proteins from the Golgi apparatus and their targeting to the vacuole. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion.
  • 基因功能參考文獻(xiàn)：
    1. Thus, Vps33/HOPS promotes productive SNARE assembly in the presence of otherwise inhibitory Sec17. PMID: 28925353
    2. Homotypic fusion and protein-sorting/class C vacuole protein-sorting (HOPS/class C Vps) complex can tether low-curvature membranes, that is, liposomes with a diameter of 100 nm, only when the yeast vacuolar Ypt7p is present in both tethered membranes. PMID: 25995379
    3. Sec17 directly promotes selective loading of Sly1 and Vps33 onto cognate SNARE complexes PMID: 24837546
    4. Vps33 appears to catalyze SNARE complex assembly through specific SNARE motif recognition. PMID: 26339030
    5. Data show that the binding sites for vacuolar SNAREs and the SNARE Vam3 Habc domain are located in the large head of the HOPS complex, where vesicular transport proteins Vps16 and Vps33 have been identified. PMID: 25564619
    6. we identify missense mutations of Vps33 that produce a novel set of defects, including cargo missorting and the loss of Vps33-HOPS association PMID: 23051737
    7. contribution of Vps33 to hemifusion and pore opening between vacuoles. Mutations in Vps33 that weaken its interactions with the SNARE complex allowed normal trans-SNARE pairing and lipid mixing but retarded content mixing. PMID: 20453860
    8. Vps33p functions at multiple trafficking steps and is not limited to action at the vacuolar membrane PMID: 15047864

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  • 亞細(xì)胞定位：
    Vacuole.
  • 蛋白家族：
    STXBP/unc-18/SEC1 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YLR396C

    STRING: 4932.YLR396C