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組蛋白修飾抗體

表觀遺傳學(xué)是研究基因表達(dá)中可遺傳的表型變化,包括修飾DNA,RNA和蛋白質(zhì),但不涉及核苷酸序列的改變。翻譯后修飾(PTM)是研究蛋白質(zhì)表觀遺傳學(xué)的最重要方法。PTM是標(biāo)記,為細(xì)胞提供廣泛的調(diào)節(jié)機(jī)制,以指示哪些基因可以關(guān)閉和打開。許多類型和家族的蛋白質(zhì)都受到PTM的影響,但得到最多修飾的蛋白質(zhì)之一是組蛋白家族。

組蛋白將基因組DNA包裝到核小體中,這使得可將大約2米的DNA放入細(xì)胞的細(xì)胞核。核小體含有兩個(gè)亞基,每個(gè)亞基由組蛋白H2A,H2B,H3和H4組成。此外,還有組蛋白H1,通常被稱為連接組蛋白。在組蛋白上發(fā)現(xiàn)的最普遍的PTM包括甲基化,乙酰化,磷酸化和泛素化,這些修飾在基因表達(dá)中至關(guān)重要,可用作打開和壓縮染色質(zhì)的信號(hào),以及促進(jìn)和拮抗轉(zhuǎn)錄的募集因子。

The Schematic of Common Histone Modification Site

這些組蛋白修飾可通過調(diào)節(jié)基因表達(dá)影響染色體功能。華美生物可提供高特異性組蛋白修飾抗體。可用于ChIP、WB、IHC、IP等多種實(shí)驗(yàn)類型。

產(chǎn)品優(yōu)勢(shì)

  • 高特異性

Western Blot analysis of Acetyl-HIST1H1C (K84) antibody (CSB-PA010378OA84acHU)

Western Blot analysis of 2-hydroxyisobutyryl-HIST1H1C (K109) Antibody (CSB-PA010378OA109hibHU)

Western Blot analysis of β-hydroxybutyryl-HIST1H3A (K18) Antibody (CSB-PA010418OA18bhbHU)

  • 可用于ChIP實(shí)驗(yàn)

Chromatin Immunoprecipitation (ChIP) analysis of Acetyl-HIST1H1C (K84) antibody (CSB-PA010378OA84acHU)

Chromatin Immunoprecipitation (ChIP) analysis of 2-hydroxyisobutyryl-HIST1H1C (K109) Antibody (CSB-PA010378OA109hibHU)

Chromatin Immunoprecipitation (ChIP) analysis of β-hydroxybutyryl-HIST1H3A (K18) Antibody (CSB-PA010418OA18bhbHU)

產(chǎn)品列表

部分熱銷組蛋白修飾抗體列表(見附表一)

靶點(diǎn) 產(chǎn)品名稱 靶點(diǎn) 產(chǎn)品名稱
H1F0 (K101) Mono-methyl-H1F0 (K101) Antibody HIST1H3A (K18) 2-hydroxyisobutyryl-HIST1H3A (K18) Antibody
H3F3A (K79) Di-methyl-H3F3A (K79) Antibody Butyrly-HIST1H3A (K18) Antibody
HIST1H1C (Ab-109) HIST1H1C (Ab-109) Antibody β-hydroxybutyryl-HIST1H3A (K18) Antibody
HIST1H1C (Ab-167) HIST1H1C (Ab-167) Antibody 2-hydroxyisobutyryl-HIST1H3A (K18) Antibody
HIST1H1C (Ab-186) HIST1H1C (Ab-186) Antibody HIST1H3A (K23) β-hydroxybutyryl-HIST1H3A (K23) Antibody
HIST1H1C (Ab-45) HIST1H1C (Ab-45) Antibody Butyrly-HIST1H3A (K23) Antibody
HIST1H1C (Ab-96) HIST1H1C (Ab-96) Antibody Propionyl-HIST1H3A (K23) Antibody
HIST1H1C (K109) Formyl-HIST1H1C (K109) Antibody HIST1H3A (K27) 2-hydroxyisobutyryl-HIST1H3A (K27) Antibody
2-hydroxyisobutyryl-HIST1H1C (K109) Antibody Acetyl-HIST1H3A (K27) Antibody
HIST1H1C (K158) 2-hydroxyisobutyryl-HIST1H1C (K158) Antibody Butyrly-HIST1H3A (K27) Antibody
HIST1H1C (K186) Mono-methyl-HIST1H1C (K186) Antibody β-hydroxybutyryl-HIST1H3A (K27) Antibody
HIST1H1C (K26) 2-hydroxyisobutyryl-HIST1H1C (K26) Antibody Tri-methyl-HIST1H3A (K27) Antibody
HIST1H1C (K45) Mono-methyl-HIST1H1C (K45) Antibody HIST1H3A (K36) Acetyl-HIST1H3A (K36) Antibody
Di-methyl-HIST1H1C (K45) Antibody Mono-methyl-HIST1H3A (K36) Antibody
HIST1H1C (K84) Acetyl-HIST1H1C (K84) Antibody Mono-methyl-HIST1H3A (K36) Antibody
HIST1H1C (K96) Acetyl-HIST1H1C (K96) Antibody Tri-methyl-HIST1H3A (K36) Antibody
Formyl-HIST1H1C (K96) Antibody HIST1H3A (K37) Acetyl-HIST1H3A (K37) Antibody
Mono-methyl-HIST1H1C (K96) Antibody HIST1H3A (K4) 2-hydroxyisobutyryl-HIST1H3A (K4) Antibody
HIST1H1D (Ab-140) HIST1H1D (Ab-140) Antibody β-hydroxybutyryl-HIST1H3A (K4) Antibody
HIST1H1E (Ab-25) HIST1H1E (Ab-25) Antibody Tri-methyl-HIST1H3A (K4) Antibody
HIST1H1E (T17) Phospho-HIST1H1E (T17) Antibody Tri-methyl-HIST1H3A (K4) Antibody
HIST1H2AG (K118) Formyl-HIST1H2AG (K118) Antibody HIST1H3A (K56) Acetyl-HIST1H3A (K56) Antibody
HIST1H2AG (K74) 2-hydroxyisobutyryl-HIST1H2AG (K74) Antibody Malonyl-HIST1H3A (K56) Antibody
Acetyl-HIST1H2AG (K74) Antibody HIST1H3A (K79) β-hydroxybutyryl-HIST1H3A (K79) Antibody
HIST1H2AG (K9) Acetyl-HIST1H2AG (K9) Antibody Butyrly-HIST1H3A (K79) Antibody
HIST1H2BC (K108) 2-hydroxyisobutyryl-HIST1H2BC (K108) Antibody HIST1H3A (K9) Acetyl-HIST1H3A (K9) Antibody
HIST1H2BC (K11) Acetyl-HIST1H2BC (K11) Antibody Crotonyl-HIST1H3A (K9) Antibody
β-hydroxybutyryl-HIST1H2BC (K11) Antibody Butyrly-HIST1H3A (K9) Antibody
HIST1H2BC (K116) Formyl-HIST1H2BC (K116) Antibody Di-methyl-HIST1H3A (K9) Antibody
Acetyl-HIST1H2BC (K116) Antibody HIST1H3A (T3) Phospho-HIST1H3A (T3) Antibody
Mono-methyl-HIST1H2BC (K116) Antibody HIST1H4A (K12) Succinyl-HIST1H4A (K12) Antibody
HIST1H2BC (K12) Acetyl-HIST1H2BC (K12) Antibody Formyl-HIST1H4A (K12) Antibody
2-hydroxyisobutyryl-HIST1H2BC (K12) Antibody Butyrly-HIST1H4A (K12) Antibody
Mono-methyl-HIST1H2BC (K12) Antibody 2-hydroxyisobutyryl-HIST1H4A (K12) Antibody
HIST1H2BC (K120) 2-hydroxyisobutyryl-HIST1H2BC (K120) Antibody HIST1H4A (K16) Butyrly-HIST1H4A (K16) Antibody
Glutaryl-HIST1H2BC (K120) Antibody Propionyl-HIST1H4A (K16) Antibody
HIST1H2BC (K15) Acetyl-HIST1H2BC (K15) Antibody 2-hydroxyisobutyryl-HIST1H4A (K16) Antibody
Mono-methyl-HIST1H2BC (K15) Antibody HIST1H4A (K20) Acetyl-HIST1H4A (K20) Antibody
HIST1H2BC (K16) Crotonyl-HIST1H2BC (K16) Antibody HIST1H4A (K31) Propionyl-HIST1H4A (K31) Antibody
HIST1H2BC (K20) Crotonyl-HIST1H2BC (K20) Antibody 2-hydroxyisobutyryl-HIST1H4A (K31) Antibody
Butyrly-HIST1H2BC (K20) Antibody Acetyl-HIST1H4A (K31) Antibody
2-hydroxyisobutyryl-HIST1H2BC (K20) Antibody HIST1H4A (K5) Butyrly-HIST1H4A (K5) Antibody
Acetyl-HIST1H2BC (K20) Antibody Propionyl-HIST1H4A (K5) Antibody
β-hydroxybutyryl-HIST1H2BC (K20) Antibody 2-hydroxyisobutyryl-HIST1H4A (K5) Antibody
Mono-methyl-HIST1H2BC (K20) Antibody β hydroxybutyryl-HIST1H4A (K5) Antibody
HIST1H2BC (K24) Acetyl-HIST1H2BC (K24) Antibody HIST1H4A (K59) Mono-methyl-HIST1H4A (K59) Antibody
HIST1H2BC (K34) 2-hydroxyisobutyryl-HIST1H2BC (K34) Antibody HIST1H4A (K77) Formyl-HIST1H4A (K77) Antibody
HIST1H2BC (K5) Butyrly-HIST1H2BC (K5) Antibody 2-hydroxyisobutyryl-HIST1H4A (K77) Antibody
HIST1H2BC (S14) Phospho-HIST1H2BC (S14) Antibody Acetyl-HIST1H4A (K77) Antibody
HIST1H3A (K115) Acetyl-HIST1H3A (K115) Antibody HIST1H4A (K79) Formyl-HIST1H4A (K79) Antibody
HIST1H3A (K122) 2-hydroxyisobutyryl-HIST1H3A (K122) Antibody HIST1H4A (K8) Butyrly-HIST1H4A (K8) Antibody
Formyl-HIST1H3A (K122) Antibody Propionyl-HIST1H4A (K8) Antibody
Acetyl-HIST1H3A (K122) Antibody 2-hydroxyisobutyryl-HIST1H4A (K8) Antibody
點(diǎn)擊下載華美生物
組蛋白修飾抗體完整目錄
HIST1H4A (K91) Acetyl-HIST1H4A (K91) Antibody
β-hydroxybutyryl-HIST1H4A (K91) Antibody

關(guān)于組蛋白修飾

組蛋白甲基化是通過添加甲基來修飾組蛋白中的某些氨基酸,繼而抑制或激活轉(zhuǎn)錄。組蛋白甲基轉(zhuǎn)移酶(HMT)通過染色質(zhì)依賴性轉(zhuǎn)錄抑制或激活來控制或調(diào)節(jié)DNA甲基化。組蛋白的甲基化和去甲基化在DNA中打開和關(guān)閉基因。

組蛋白磷酸化是指激酶使組蛋白上的特異性絲氨酸、蘇氨酸或酪氨酸磷酸化,并且可通過磷酸酶進(jìn)行去磷酸化。磷酸化通常發(fā)生在DNA修復(fù)和有絲分裂期間,另外在細(xì)胞分裂和細(xì)胞凋亡過程的DNA修復(fù)、轉(zhuǎn)錄和染色質(zhì)整合中起重要的作用。

組蛋白乙?;?/strong>主要發(fā)生在H3、H4的N端相對(duì)保守的賴氨酸位置上,是由組蛋白乙酰轉(zhuǎn)移酶和組蛋白去乙?;竻f(xié)調(diào)進(jìn)行。該過程與許多細(xì)胞過程的調(diào)控密切相關(guān),包括染色質(zhì)動(dòng)力學(xué)和轉(zhuǎn)錄,基因沉默,細(xì)胞周期進(jìn)程,細(xì)胞凋亡、分化、DNA復(fù)制、DNA修復(fù)、核輸入和神經(jīng)元抑制。

組蛋白琥珀酰化是一種翻譯后修飾,將琥珀酰基(-CO-CH2 -CH2 -COOH)加到組蛋白的賴氨酸殘基上。琥珀?;臐撛谧饔谜谘芯恐校S著琥珀?;募尤?,賴氨酸的電荷從+1變?yōu)?1(在生理pH下)并引入一個(gè)100Da結(jié)構(gòu),明顯大于乙?;?2Da)或甲基化(14 Da),研究人員推測(cè)這將會(huì)使蛋白質(zhì)結(jié)構(gòu)和功能發(fā)生更顯著的變化。

組蛋白2-羥基異丁?;?/strong>是一種新型的組蛋白標(biāo)記,并已在63個(gè)人和小鼠組蛋白的Khib位點(diǎn)得到鑒定,包括27個(gè)獨(dú)特的賴氨酸位點(diǎn)。該組蛋白標(biāo)記最初由MS鑒定,然后通過化學(xué)和生物化學(xué)方法驗(yàn)證。

組蛋白生物素化是一個(gè)相對(duì)較新的研究領(lǐng)域,組蛋白生物素化的生物學(xué)作用證據(jù)很少。最新研究表明,組蛋白生物素化可以增強(qiáng)人淋巴細(xì)胞中細(xì)胞增殖的反應(yīng)。此外,組蛋白生物素化也可能在細(xì)胞對(duì)DNA損傷的反應(yīng)中起重要作用。

組蛋白甲?;?/strong>也是翻譯后修飾之一,可以調(diào)節(jié)染色質(zhì)構(gòu)象和基因激活。目前已經(jīng)在組蛋白中賴氨酸殘基的Nε上得到鑒定。另外也在接頭組蛋白和高遷移率族蛋白中觀察到這種修飾。

組蛋白巴豆?;?/strong>是一種新確定的組蛋白修飾,與哺乳動(dòng)物細(xì)胞中的活躍轉(zhuǎn)錄有關(guān)。趙英明教授實(shí)驗(yàn)室于2011年發(fā)現(xiàn)一種新的賴氨酸殘基組蛋白修飾物,該修飾與基因表達(dá)相關(guān),并且標(biāo)記了啟動(dòng)子和增強(qiáng)子,類似于乙?;?,后命名為巴豆?;?。

組蛋白羥基化是將CH基團(tuán)轉(zhuǎn)化為COH基團(tuán)的許多翻譯后修飾之一。羥基化是一種氧化過程。一些研究表明,組蛋白5-羥基化可能在基因轉(zhuǎn)錄或染色體重排的表觀遺傳調(diào)控中起重要作用。

組蛋白丙二?;?/strong>是一種新型的組蛋白PTM。相關(guān)研究表明,與賴氨酸乙?;啾龋嚢彼岜;瘜д姷臍埢?yōu)樨?fù)電荷,這將會(huì)引起更顯著的結(jié)構(gòu)變化。

組蛋白戊二酸化通常發(fā)生在賴氨酸上。Kristin A. Anderson等人已經(jīng)鑒定并全面驗(yàn)證賴氨酸戊二酸化在進(jìn)化過程中屬于保守的PTM,主要影響代謝過程和其他線粒體功能。

組蛋白β-羥基丁基化是一種新型的組蛋白修飾,通常也發(fā)生在賴氨酸上。謝中宇團(tuán)隊(duì)的研究表明,小鼠肝臟長時(shí)間禁食會(huì)顯著誘導(dǎo)β-羥基丁基化,與饑餓反應(yīng)代謝途徑中表達(dá)上調(diào)的基因相關(guān)。