Recombinant Mouse 5'-nucleotidase (Nt5e), partial (Active)
In Stock-
中文名稱:小鼠Nt5e重組蛋白
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貨號:CSB-AP005101MO
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規(guī)格:¥1740
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圖片:
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其他:
產(chǎn)品詳情
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純度:Greater than 95% as determined by SDS-PAGE.
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內毒素:Less than 1.0 EU/μg as determined by LAL method.
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生物活性:Measured by its binding ability in a functional ELISA. Immobilized Mouse CD73-His at 2μg/ml can bind Anti-Human CD73 mAb, the ED50 of Anti-Human CD73 mAb is 3.70 ng/ml.
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基因名:Nt5e
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Uniprot No.:
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別名:Nt5e; Nt5; Nte5'-nucleotidase; 5'-NT; EC 3.1.3.5; Ecto-5'-nucleotidase; CD antigen CD73
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種屬:Mus musculus (Mouse)
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蛋白長度:Partial
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來源:Mammalian cell
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分子量:58.8 kDa
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表達區(qū)域:29-550aa
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氨基酸序列WELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLFLDAGDQYQGTIWFTVYKGLEVAHFMNILGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAHQISGLFLPSKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEISALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDIVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTADDGRQVPVVQAYAFGKYLGYLKVEFDDKGNVITSYGNPILLNSSIPEDATIKADINQWRIKLDNYSTQELGRTIVYLDGSTQTCRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDEKNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDINRKPWNRVVQLEVLCTKCRVPIYEPLEMDKVYKVTLPSYLANGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVVYPAVEGRIKF
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蛋白標簽:C-terminal 6xHis-tagged
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產(chǎn)品提供形式:Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
緩沖液:0.2 μm Filtered 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl2, 20% Glycerol, pH 7.5
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儲存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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Datasheet & COA:Please contact us to get it.
相關產(chǎn)品
靶點詳情
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功能:Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
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基因功能參考文獻:
- Deletion of CD73 in mice leads to aortic valve dysfunction similar to that induced by high-fat diet suggesting important role of this surface protein in maintaining heart valve integrity. PMID: 28192180
- We establish that the mouse Nt5e minimal promoter transcriptional activity is negatively regulated by an Elf2-like Ets-related transcription factor in activated mouse liver myofibroblasts PMID: 28667437
- CD73 derived adenosine production slows down migration of Langerin+ dendritic cells from skin to lymph nodes. This may be a crucial mechanism to avoid over boarding immune reactions against haptens. PMID: 28743609
- Compared to adults, young WT mice failed to control S. pneumoniae infection after vaccination and this was associated with lower levels of CD73 on innate B cells. We hypothesized that pharmacological activation of A2a receptor may improve Pneumovax 23 immunization in young WT mice PMID: 29377929
- The 5'-ectonucleotidase CD73 has a key role in regulating macrophage immune responses during efferocytosis by converting AMP released from apoptotic cells into adenosine to suppress multiple pro-inflammatory cytokines. PMID: 28060378
- findings demonstrate the critical role of CD73 in the regulation of infection by the enteric pathogen Salmonella and implicate adenosine as an important host-derived factor that mediates host-microbe interaction PMID: 28717030
- Data show that AMP deaminase 3 (Ampd3)-/-/5'-nucleotidase (CD73)-/- knockout mice are more sensitive to AMP-induced hypometabolism than mice with a single enzyme deficiency, which are more sensitive than wild type. PMID: 28746349
- This study evaluated the role of CD73 in the accumulation and adhesion of pulmonary macrophages in response to thoracic irradiation. PMID: 28325757
- Deletion of CD39 and CD73 or both caused an inhibition of the microglia ramified phenotype in the brain with a reduction in the length of processes, branching frequency and number of intersections with Sholl spheres. In vitro, unlike wild-type microglia, cd39-/- and cd73-/- microglial cells were less complex and did not respond to ATP with the transformation into a more ramified phenotype. PMID: 28376099
- CD73 expression is regulated during experimental autoimmune encephalomyelitis, this enzyme is not absolutely required to either promote or limit Th17 cell expansion or experimental autoimmune encephalomyelitis severity. PMID: 28288184
- High NT5E expression is associated with Radiation-Induced Lung Fibrosis. PMID: 26921334
- Ablation of CD73 minimally effects in vivo thrombosis, but increased CD39 expression on hematopoietic-derived cells, especially monocytes, attenuates in vivo arterial thrombosis. PMID: 27417582
- CD73 on T cells plays an important anti-inflammatory role in transverse aortic constriction-induced heart failure PMID: 28404626
- In this study, we investigated whether blockade of the A2A adenosine receptor with a selective antagonist and a CD73 inhibitor may increase the efficacy of a dendritic cell-based cancer vaccine PMID: 28349824
- Data indicate that adenosine and CGS21680 upregulate CD39 and CD73 via E2F-1 and CREB. PMID: 27430240
- CD73-siRNA-loaded ChLa NPs may be considered as a promising therapeutic tool for cancer therapy; however, further in vivo investigations are necessary PMID: 26733167
- CD73/adenosine pathway involves the immunomodulatory function of mesenchymal stem cells in autoimmune responses. PMID: 26650818
- CD73 facilitates AS by promoting migration, proliferation, and foam cell transformation of vascular smooth muscle cells and elevating serum lipid levels. PMID: 26506509
- Low CD73 expression plays a role in melanoma progression. PMID: 26545615
- These data reveal a novel function of CD73 ectonucleotidase in arresting CD8(+) T-cell differentiation and support the idea that CD73-driven adenosine production by Tc17 cells may promote stem cell-like properties in Tc17 cells. PMID: 26331349
- gammadelta T cells expressed different CD73 amounts in different stages of experimental autoimmune uveitis. Low CD73 on gammadelta T cells enhanced Th17 response-promoting activity. Failure to express CD73 decreased enhancing and suppressive effects of gammadelta T cells. PMID: 26919582
- Activation of A3, A2A and A1 Adenosine Receptors in CD73-Knockout Mice Affects B16F10 Melanoma Growth, Neovascularization, Angiogenesis and Macrophage Infiltration PMID: 26964090
- CD73 activity from any host cell type is not required for the monocyte/macrophage polarization in the peritoneum towards a pro- or an anti-inflammatory phenotype in vivo PMID: 26258883
- CD73 is not necessary for development of acute lung injury following influenza A virus infection. PMID: 26432867
- The ecto-5'-nucleotidase derived extracellular formation of adenosine does not contribute substantially to adenosine's well known cardioprotective effect in early phase ischemic preconditioning. PMID: 26261991
- CD73 expression was downregulated during acute infection with T. gondii, leading to impaired capacity to produce adenosine. PMID: 25389034
- Expression of NPP1 and 5'-nucleotidase by valve interstitial cells promotes the mineralization of the aortic valve through A2aR and a cAMP/PKA/CREB pathway. PMID: 25644539
- The CD73 deficiency significantly increased the levels of proinflammatory cytokines in mice. PMID: 25805696
- our results support the view that CD73 fine-tunes antimycobacterial immune responses. PMID: 26150535
- CD73 expression attenuates inflammation during murine Salmonellosis and impairs immunity, leading to increased bacterial colonization and prolonged infection. PMID: 24670982
- CD73 is dispensable for normal CD8+ T-cell differentiation and function PMID: 25490556
- The mouse model deficient in CD73, with other targeted mutant mice with vascular mineralization, attests to the presence of a complex pro-mineralization/anti-mineralization network that prevents ectopic tissue mineralization. PMID: 25486201
- In the urothelium of animals with bladder cancer there was an increase in the expression of ecto-5'-nucleotidase. PMID: 24464643
- Data show that CD73 (5' nucleotidase) deficiency resulted in greater disease incidence. PMID: 25681339
- the absence of CD73-generated adenosine led to the increased susceptibility to Toxoplasma gondii infection in CD73 knockout mice. PMID: 25452548
- CD73 enhances endothelial barrier function and sprouting in blood but not lymphatic vasculature. PMID: 25402681
- Tetraspanin CD9 and ectonucleotidase CD73 identify an osteochondroprogenitor population with elevated osteogenic properties. PMID: 25564652
- CD73 and TNAP play interactive roles to metabolize luminally applied 5'-AMP in the renal vasculature such that inhibition of both is required to inhibit the production of adenosine. PMID: 24990899
- CD73-dependent adenosine signaling is prominent in the mature GC and required for establishment of the long-lived PC compartment, thus identifying a novel role for CD73 in humoral immunity PMID: 24664100
- that extracellular adenosine, generated in tandem by ecto-enzymes CD39 and CD73, promotes dermal fibrogenesis. PMID: 24266925
- CD73-dependent production of extracellular adenosine and endothelial Adora2b signaling protects kidney during diabetic nephropathy. PMID: 24262796
- Isoflurane causes TGF-beta1-dependent induction of renal tubular CD73 and adenosine generation to protect against renal ischemia and reperfusion injury. PMID: 23423261
- identify CD73 as a TCR ligand-induced cell surface protein that distinguishes gammadeltaTCR-expressing CD4(-)CD8(-) progenitors that have committed to the gammadelta fate from those that have not yet done so PMID: 24493796
- CD73 mRNA levels were also dramatically decreased in human liver biopsies from hepatitis C and nonalcoholic fatty liver disease patients PMID: 23729294
- Data indicate that CD73 promoted metastasis through the activation of both A2A and A2B receptors. PMID: 23964122
- Our data provide evidence for a role of CD73 in the regulation of learning and memory and psychomotor coordination PMID: 23274765
- findings demonstrate that CD73-expressing exosomes produced by Treg cells following activation contribute to their suppressive activity through the production of adenosine PMID: 23749427
- Our novel findings suggest an important immune-regulatory role of CD73 in regulation of diabetes development PMID: 23956420
- NT5E (and prostatic acid phosphatase) are the main AMP ectonucleotidases in somatosensory neurons. PMID: 23825434
- CD73 is upregulated in immune cells after ischemia/reperfusion in the myocardium. PMID: 23720442
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亞細胞定位:Cell membrane; Lipid-anchor, GPI-anchor.
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蛋白家族:5'-nucleotidase family
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