Bovine Vitamin A (VA) ELISA Kit

產(chǎn)品詳情

說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

VA（Vitronectin）是一種在體內(nèi)廣泛存在的糖蛋白，與許多生物學(xué)過(guò)程有關(guān)，如凝血、細(xì)胞外基質(zhì)的重構(gòu)以及炎癥反應(yīng)等。VA可以結(jié)合到細(xì)胞表面上的特定受體中，參與了許多信號(hào)通路的調(diào)控。在一些疾病中，VA的含量和活性也發(fā)生了改變，如某些癌癥和心血管疾病

華美生物所提供的Bovine Vitamin A (VA) ELISA Kit屬于ELISA檢測(cè)試劑盒，采用競(jìng)爭(zhēng)法定量檢測(cè)牛血清、血漿、組織勻漿樣本中的VA，其靈敏度為4.672 ng/mL，檢測(cè)范圍為6.25 ng/mL-100 ng/mL。
縮寫：

VA
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

6.25 ng/mL-100 ng/mL
靈敏度：

4.672 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine VA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	95
	Range %	91-99
1:20	Average %	103
	Range %	99-107
1:40	Average %	98
	Range %	93-103
1:80	Average %	92
	Range %	89-95

回收率：

The recovery of bovine VA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	93-104
EDTA plasma (n=4)	89	83-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究進(jìn)展：

最新研究表明，VA蛋白在細(xì)胞分化、免疫應(yīng)答和炎癥反應(yīng)等生物學(xué)過(guò)程中起著重要的作用。