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Chicken Interleukin 12,IL-12/70 ELISA Kit

  • 中文名稱:
    雞白介素12(IL-12/70)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E12836C
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:

    This Chicken IL12/70 ELISA Kit was designed for the quantitative measurement of Chicken IL12/70 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.78 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

  • 縮寫:
    IL-12/70
  • 種屬:
    Gallus gallus (Chicken)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.78 pg/mL-200 pg/mL
  • 靈敏度:
    0.78 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-12/70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 88-98  
    1:2 Average % 94  
    Range % 90-98  
    1:4 Average % 92  
    Range % 87-98  
    1:8 Average % 96  
    Range % 92-101  
  • 回收率:
    The recovery of chicken IL-12/70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 91-99  
    EDTA plasma (n=4) 92 88-97  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.192 0.188 0.190    
    50 0.273 0.281 0.277    
    12.5 0.585 0.565 0.575    
    3.12 0.876 0.824 0.850    
    0.78 1.424 1.457 1.441    
    0 2.265 2.233 2.249    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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