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  4. Fish Insulin,INS ELISA Kit

Fish Insulin,INS ELISA Kit

  • 中文名稱：
    魚胰島素(INS)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12123Fh
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Fish INS ELISA Kit was designed for the quantitative measurement of Fish INS protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 100 pg/mL-1600 pg/mL and the sensitivity is 100 pg/mL.

  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Fish
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    100 pg/mL-1600 pg/mL
  • 靈敏度：
    100 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 86-100  
    1:2 Average % 98  
    Range % 92-103  
    1:4 Average % 85  
    Range % 80-89  
    1:8 Average % 91  
    Range % 83-99  
  • 回收率：
    The recovery of fish INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 88-98  
    EDTA plasma (n=4) 92 90-95  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1600 0.178 0.189 0.184    
    800 0.322 0.340 0.331    
    400 0.610 0.634 0.622    
    200 1.257 1.230 1.244    
    100 2.335 2.475 2.405    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

問答及客戶評論

■ 常見問題解答
Q:

我想找一款可以檢測斑馬魚胰島素水平的ELISA試劑盒。你有推薦的么？

A:
我們有以下魚試劑盒： #CSB-E12123Fh，魚胰島素，INS ELISA Kit 一般來說，同一物種間的蛋白質(zhì)差異不大。也就是說，不同類型魚類的氨基酸序列相對保守 這個項目#CSB-E12123Fh是專門針對鮭魚的。我們沒有測試過斑馬魚。但應該沒有什么大問題，你可以試著先做一個預實驗。