Fish growth hormone,GH ELISA Kit
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中文名稱:
魚生長激素(GH)酶聯(lián)免疫試劑盒
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貨號:
CSB-E12121Fh
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規(guī)格:
96T/48T
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價格:
¥4200/¥3000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Fish growth hormone (GH) ELISA Kit was designed for the quantitative measurement of Fish growth hormone (GH) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 312.5 pg/mL-5000 pg/mL and the sensitivity is 312.5 pg/mL.
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別名:
gh1 ELISA Kit; Somatotropin-1 ELISA Kit; Growth hormone I ELISA Kit; Somatotropin I ELISA Kit
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縮寫:
GH
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Uniprot No.:
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種屬:
Fish
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樣本類型:
serum, plasma, tissue homogenates
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檢測范圍:
312.5 pg/mL-5000 pg/mL
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靈敏度:
312.5 pg/mL
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反應(yīng)時間:
1-5h
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樣本體積:
50-100ul
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檢測波長:
450 nm
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研究領(lǐng)域:
Developmental Biology
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測定原理:
quantitative
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測定方法:
Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% |
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Three samples of known concentration were tested twenty times on one plate to assess. |
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Inter-assay Precision (Precision between assays): CV%<15% |
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Three samples of known concentration were tested in twenty assays to assess. |
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of fish GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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1:1 |
Average % |
95 |
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Range % |
91-98 |
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1:2 |
Average % |
92 |
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Range % |
88-96 |
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1:4 |
Average % |
102 |
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Range % |
96-106 |
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1:8 |
Average % |
96 |
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Range % |
92-101 |
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回收率:
The recovery of fish GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
Sample Type |
Average % Recovery |
Range |
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Serum (n=5) |
95 |
92-98 |
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EDTA plasma (n=4) |
94 |
90-98 |
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
pg/ml |
OD1 |
OD2 |
Average |
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5000 |
0.271 |
0.265 |
0.268 |
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2500 |
0.526 |
0.514 |
0.520 |
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1250 |
0.887 |
0.863 |
0.875 |
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625 |
1.664 |
1.684 |
1.674 |
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312.5 |
2.557 |
2.602 |
2.580 |
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本試劑盒所含材料:
- A 96-well Assay plate --The 96-well plate has been pre-coated with anti-Fish GH antibody.
- Standard (6 x 1 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- HRP-avidin (1 x 6 ml) --Catalyze the TMB to elicit a chromogenic reaction.
- Biotin-conjugated GH (1 x 6 ml) --Bind to the precoated antibody and act as the detection antigen.
- Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
- Wash Buffer (20x concentrate) (1 x 15 ml) --Wash away unbound or free substances.
- Substrate A (1x 7 ml) --Mix with Sbustrate B.
- Substrate B (1x 7 ml) --Mix with Sbustrate A
- Stop Solution (1 x 7ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)
- An Instruction manual
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本試劑盒不含材料:
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
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數(shù)據(jù)處理:
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貨期:
3-5 working days
引用文獻(xiàn)
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Integrated biofloc technology in red tilapia aquaculture: Salinity-dependent effects on water quality, parental stock physiology, reproduction, and immune responses
GR Sallam,Aquaculture International,2024
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Progressive substitution of fish oil with Schizochytrium-derived algal oil in the diet of Atlantic salmon (Salmo salar) parr subjected to winter signal period
NW Farris,Aquaculture Reports,2024
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Effects of dietary supplementation of Bacillus, β-glucooligosaccharide and their synbiotic on the growth, digestion, immunity, and gut microbiota profile of abalone, Haliotis discus hannai
J Cadangin,Aquaculture Reports,2024
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Tissue explants as tools for studying the epigenetic modulation of the GH-IGF-I axis in farmed fish
E Perera,Frontiers in physiology,2024
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Raw Fish-based Moist Pellet and Extruded Pellet on Growth, Levels and Expression of GH/IGF Axis, Intestinal Enzyme Activity and Gastrointestinal Morphology of …
CY Jeon,/,2023
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Potential role of dietary white poplar (Populus alba L.) in stimulating growth, digestion, and antioxidant/immune status of Nile tilapia (Oreochromis niloticus)
RE Ibrahim,/,2024
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TNF-Α, IL-6, HSP-70, fish growth hormone, and growth performance of sea trout, Salmo trutta (Actinopterygii: Salmoniformes: Salmonidae) after long-term dietary administration of β-glucan and BGN-2
O Revina,/,2023
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Effects on Growth Hormone, Insulin-Like Growth Factors, and Oxidative Stress in Zebrafish Larvae after Exposure to Acetyl Tributyl Citrate and Acetyl Triethyl Citrate
K Yun,Environmental pollution,2023
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Combined Effects of Preservative Chemicals in Consumer Products Using Embryonic and Adult Zebrafish
C Park,/,2023
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Effects of Stocking Density on Stress, Hematological Responses, and Growth of Black Rockfish Sebastes schlegelii
JW Lee,Journal of aquatic animal health,2023
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The Combined Effects of Preservative Chemicals in Consumer Products: An Analysis Using Embryonic and Adult Zebrafish
T Wang,/,2024
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Fibrinogen beta chain may be a potential predict biomarker for pre-eclampsia: a preliminary study
J Shi,Animals,2022
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Interaction of Low-level Dietary Supplementation of Chlorella vulgaris and
Feeding Duration on Growth Hormone, Growth Performance and
Biochemical Indices of Red Hybrid Tilapia
MT Ahmad,researchsquare,2022
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Schizochytrium-derived algal oil as replacement for fish oil-effect on growth and smoltification process of Atlantic salmon (Salmo salar L.)
DH Blaauw,Nord universitet,2022
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Expression of insulin‐like growth factor genes in olive flounder, Paralichthys olivaceus, fed a diet with partial replacement of dietary fish meal
JS Moon,Journal of the World Aquaculture Society,2022
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Effects of tetramethyl bisphenol F on thyroid and growth hormone-related endocrine systems in zebrafish larvae
H Kim,Ecotoxicology and environmental safety,2022
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A preliminary study of dietary protein requirement of juvenile marbled flounder (Pseudopleuronectes yokohamae)
JH Cho,Animal Nutrition,2021
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The IGF-1/GH-GLUTs-plasma glucose regulating axis in hybrid grouper (Epinephelus fuscoguttatus♀ × epinephelus lanceolatus♂) fed a high-carbohydrate diet
Xiaoyi Wu,General And Comparative Endocrinology,2021
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The effects of four plant extracts on growth, sex reversal, immunological and haemato‐biochemical parameters in Nile tilapia, Oreochmomis niloticus (Linnaeus, 1758)
I Ghosal,Aquaculture Research,2020
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Induction of immunological, hormonal and histological alterations after sublethal exposure of chlorpyrifos in the freshwater fish, Pseudetroplus maculatus (Bloch, 1795 …
KP Raibeemol,Fish & Shellfish Immunology,2020
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Impact of temperature shift on gill physiology during smoltification of Atlantic salmon smolts (Salmo salar L.)
B Bernard,Comp. Biochem. Physiol., Part A Mol. Integr. Physiol,2020
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Dietary administration of astaxanthin improves feed utilization, growth performance and survival of Asian seabass, Lates calcarifer (Bloch, 1790)
Keng Chin Lim, et al,Aquaculture Nutrition,2019
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A temperature shift on the migratory route similarly impairs hypo-osmoregulatory capacities in two strains of Atlantic salmon (Salmo salar L.) smolts
Bernard B,Fish Physiology and Biochemistry,2019
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Phenotypic plasticity as an important mechanism of cave colonization and adaptation in Astyanax cavefish
Helena Bilandzija,bioRxiv,2019
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PERTUMBUHAN IKAN BANDENG (Chanos chanos) ANTARA BENIH HATCHERY SKALA RUMAH TANGGA DAN GENERASI KEDUA (G-2) TERSELEKSI
Daniar Kusumawati.et al,/,2018
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Changes of Somatotropin Concentration in Blood Serum of Juvenile Russian Sturgeon Acipenser gueldenstaedtii (Acipenseriformes) During Adaptation to Hyperosmotic Medium
O. G. Semenova.et al,Journal of Ichthyology,2018
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THE EFFECTS OF PESTICIDE NEEMAZAL ON THE GROWTH HORMONE (GH) AND INSULIN-LIKE GROWTH HORMONE (IGF-1) OF CARP FISH (Cyprinus
Tihomir Dovramadjiev.et al,researchgate,2016
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Molecular characterization, tissue distribution, and expression regulation from fasting and re-feeding of two growth hormone receptors in mandarin fish Siniperca chuatsi
X Lu. et al,Original Article Chemistry And Biochemistry,2015
靶點(diǎn)詳情
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功能:
Growth hormone plays an important role in growth control and is involved in the regulation of several anabolic processes. Implicated as an osmoregulatory substance important for seawater adaptation.
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亞細(xì)胞定位:
Secreted.
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蛋白家族:
Somatotropin/prolactin family
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