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Goat Tri-iodothyronine(T3) ELISA KIT

  • 中文名稱:
    山羊三碘甲狀腺原氨酸(T3)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E13385G
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
        T3是一種甲狀腺激素,對(duì)于機(jī)體的代謝和發(fā)育有重要影響。它通過結(jié)合甲狀腺激素受體,影響基因轉(zhuǎn)錄和蛋白質(zhì)合成。T3水平的調(diào)節(jié)對(duì)于機(jī)體內(nèi)平衡的維持至關(guān)重要,過低或過高的T3水平都會(huì)對(duì)身體產(chǎn)生負(fù)面影響。因此,T3在甲狀腺功能異常、代謝異常等疾病的診斷和治療中具有重要作用。
        華美生物所提供的Goat Tri-iodothyronine(T3) ELISA KIT屬于ELISA檢測(cè)試劑盒,采用競(jìng)爭(zhēng)法定量檢測(cè)山羊血清、血漿、組織勻漿樣本中的T3,其靈敏度為0.5 ng/mL,檢測(cè)范圍為0.5 ng/mL-8 ng/mL。
     
  • 縮寫:
    T3
  • 種屬:
    Capra hircus (Goat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    0.5 ng/mL-8 ng/mL
  • 靈敏度:
    0.5 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of goat T3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 95    
    Range % 90-103    
    1:2 Average % 95    
    Range % 85-102    
    1:4 Average % 93    
    Range % 90-97    
    1:8 Average % 94    
    Range % 88-99    
  • 回收率:
    The recovery of goat T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 97 86-102    
    EDTA plasma (n=4) 96 92-100    
                   
                   
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    8 0.166 0.168 0.167      
    4 0.281 0.288 0.285      
    2 0.558 0.553 0.556      
    1 1.262 1.247 1.255      
    0.5 2.105 2.138 2.122      
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

問答及客戶評(píng)論

 常見問題解答
Q:

I'm studying metabolic stress in dairy goats in the peripartum.
In total, 450 samples have to be analysed for each hormone, and analysis will be done in duplicate. Meaning I'll need a number of kits enough to run 900 tests.
Could you please send me a quote specifying the plate size (i.e. 96 well) and the number of free wells per plate. (i.e. 96 well minus the number of wells used for the standard curve).

A:
Thanks for your inquiry. We have the mentioned kit available.For example:
#CSB-E13385G,Goat Tri-iodothyronine(T3) ELISA KIT *96T (If duplicate done,22 kits is advised;If not,only 11 kits is ok)
the standard wells are S1-S5 and duplicate is advised.With a blank well,the number of standard wells is 5×2+1=11.The rest is for samples wells,85=96-11(If duplicate is done for sample,then the number will be the half of that.)
If you want to detect diary goat samples,we suggest you do pre-testing first.

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    近期研究表明,T3在許多重要的生理過程中扮演著關(guān)鍵角色,如代謝調(diào)節(jié)、心臟功能、神經(jīng)發(fā)育和免疫系統(tǒng)等。此外,一些研究還發(fā)現(xiàn),T3可能在腫瘤生長(zhǎng)和轉(zhuǎn)移中發(fā)揮一定作用,值得深入探究。