Goat gonadotropin-releasing hormone,GnRH ELISA Kit

產(chǎn)品詳情

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

This Goat GnRH ELISA Kit was designed for the quantitative measurement of Goat GnRH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 2.5 pg/mL-500 pg/mL and the sensitivity is 1.25 pg/mL .
縮寫：

GnRH
Uniprot No.：

D3YJ02
種屬：

Capra hircus (Goat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

2.5 pg/mL-500 pg/mL
靈敏度：

1.25 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-99
1:2	Average %	95
	Range %	88-102
1:4	Average %	94
	Range %	89-99
1:8	Average %	95
	Range %	89-103

回收率：

The recovery of goat GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	88-102
EDTA plasma (n=4)	97	91-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.