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中文名稱:山羊促性腺激素釋放激素(GnRH)酶聯(lián)免疫試劑盒
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貨號:CSB-E13276G
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規(guī)格:96T/48T
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價格:¥4200/¥3000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Goat GnRH ELISA Kit was designed for the quantitative measurement of Goat GnRH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 2.5 pg/mL-500 pg/mL and the sensitivity is 1.25 pg/mL .
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縮寫:GnRH
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Uniprot No.:
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種屬:Capra hircus (Goat)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:2.5 pg/mL-500 pg/mL
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靈敏度:1.25 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of goat GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 90-99 1:2 Average % 95 Range % 88-102 1:4 Average % 94 Range % 89-99 1:8 Average % 95 Range % 89-103 -
回收率:
The recovery of goat GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 88-102 EDTA plasma (n=4) 97 91-104 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 0 2.557 2.458 2.508 2.5 1.495 1.481 1.488 10 1.053 1.045 1.049 40 0.650 0.636 0.643 125 0.408 0.392 0.400 500 0.222 0.214 0.218 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- Effect of central administration of indomethacin on anandamide-induced GnRH/LH secretion in the hypothalamus of anoestrous ewes D Tomaszewska-Zaremba,Journal of Veterinary Research,2024
- Does kisspeptin participate in GABA-mediated modulation of GnRH and GnRH receptor biosynthesis in the hypothalamic-pituitary unit of follicular-phase ewes? Magdalena OlgaCiechanowska, et al,Pharmacological reports,2019
- Effect of CD14/TLR4 antagonist on GnRH/LH secretion in ewe during central inflammation induced by intracerebroventricular administration of LPS Karolina Haziak.et al,Journal of Animal Science and Biotechnology,2018
- Hypothalamic-pituitary GnRH/LH axis activity is affected by salsolinol in sheep during lactation: Effects of intracerebroventricular infusions of salsolinol and its antagonizing analogue. Marciniak E.et al,Theriogenology.,2016
- Central Injection of Exogenous IL-1ß in the Control Activities of Hypothalamic??ituitary??onadal Axis in Anestrous Ewes AP Herman et al,Reproduction in Domestic Animals,2012
- The effect of rivastigmine on the LPS-induced suppression of GnRH/LH secretion during the follicular phase of the estrous cycle in ewes Herman AP et al,Anim Reprod Sci.,2013
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