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Goat vitamin B12 (VB12) ELISA kit

  • 中文名稱:
    山羊維生素B12(VB12)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EQ028114GO
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:

    This Goat VB12 ELISA Kit was designed for the quantitative measurement of Goat VB12 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.

  • 縮寫:
    VB12
  • 種屬:
    Capra hircus (Goat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    1.56 pg/mL-100 pg/mL
  • 靈敏度:
    0.39 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of goat VB12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:10 Average % 85
    Range % 80-92
    1:20 Average % 97
    Range % 90-105
    1:40 Average % 97
    Range % 92-104
    1:80 Average % 92
    Range % 86-98
  • 回收率:
    The recovery of goat VB12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 89-100
    EDTA plasma (n=4) 97 90-100
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average  
    100 0.145 0.148 0.147  
    50 0.221 0.227 0.224  
    25 0.396 0.394 0.395  
    12.5 0.837 0.815 0.826  
    6.25 1.252 1.243 1.248  
    3.12 2.018 2.002 2.010  
    1.56 2.679 2.641 2.660  
    0 2.754 2.716 2.735  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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