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  4. Human C-C motif chemokine 27 (CCL27) ELISA Kit

Human C-C motif chemokine 27 (CCL27) ELISA Kit

  • 中文名稱：
    人C-C型趨化因子27(CCL27)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E09125h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
        CCL27，也稱為皮膚趨化蛋白-3（CTACK），是C-C趨化因子家族中的一種趨化因子。它主要由表皮細(xì)胞和角質(zhì)形成細(xì)胞等皮膚細(xì)胞產(chǎn)生，并且能夠吸引CCR10受體表達(dá)的細(xì)胞，如Th1和Th2細(xì)胞等。CCL27在皮膚免疫應(yīng)答中發(fā)揮著重要作用，如過(guò)敏性皮膚炎、銀屑病等。
        華美生物所提供的Human C-C motif chemokine 27 (CCL27) ELISA Kit屬于ELISA檢測(cè)試劑盒，采用雙抗夾心法定量檢測(cè)人血清、血漿、組織勻漿樣本中的CCL27，其靈敏度為0.043 ng/mL，檢測(cè)范圍為0.313 ng/mL-20 ng/mL。
     
  • 別名：
    ALP ELISA Kit; C-C motif chemokine 27 ELISA Kit; CC chemokine ILC ELISA Kit; Ccl27 ELISA Kit; CCL27_HUMAN ELISA Kit; Chemokine (C-C motif) ligand 27 ELISA Kit; CTACK ELISA Kit; CTAK ELISA Kit; Cutaneous T cell attracting chemokine ELISA Kit; Cutaneous T-cell-attracting chemokine ELISA Kit; ESkine ELISA Kit; IL 11 R alpha locus chemokine ELISA Kit; IL-11 R-alpha-locus chemokine ELISA Kit; ILC ELISA Kit; PESKY ELISA Kit; SCYA27 ELISA Kit; Skinkine ELISA Kit; Small inducible cytokine A27 ELISA Kit; small inducible cytokine subfamily A (Cys-Cys); member 27 ELISA Kit; Small-inducible cytokine A27 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.313 ng/mL-20 ng/mL
  • 靈敏度：
    0.043 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CCL27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 85    
    Range % 81-88    
    1:2 Average % 95    
    Range % 90-101    
    1:4 Average % 104    
    Range % 101-107    
    1:8 Average % 89    
    Range % 85-93    
  • 回收率：
    The recovery of human CCL27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 97 94-100    
    EDTA plasma (n=4) 105 101-109    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    20 2.715 2.768 2.742 2.589    
    10 2.097 2.014 2.056 1.903    
    5 1.276 1.234 1.255 1.102    
    2.5 0.752 0.768 0.760 0.607    
    1.25 0.481 0.498 0.490 0.337    
    0.625 0.327 0.310 0.319 0.166    
    0.313 0.257 0.244 0.251 0.098    
    0 0.156 0.150 0.153 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
        CCL27是一種趨化因子，主要由樹突狀細(xì)胞和其他免疫細(xì)胞產(chǎn)生，可通過(guò)介導(dǎo)免疫細(xì)胞的趨化和激活來(lái)參與多種疾病的免疫調(diào)節(jié)。最近的研究表明，CCL27在某些腫瘤中表達(dá)水平升高，可能與腫瘤的生長(zhǎng)和擴(kuò)散相關(guān)。此外，CCL27也被發(fā)現(xiàn)參與皮膚病理生理過(guò)程的調(diào)節(jié)。
     
  • 功能：
    Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
  • 基因功能參考文獻(xiàn)：
    1. Plasma CCL27 levels were significantly lower in the VCA-IgA-negative normal subjects than in either the VCA-IgA-positive healthy donorsor the NPC patients. PMID: 29295705
    2. CCR10/CCL27 crosstalk mediated drug resistance, contributing to failure of proteasome-inhibitors in multiple myeloma. PMID: 27732933
    3. detected in the nucleus in eczema, cytoplasm in psoriasis PMID: 27193975
    4. CCL27 drives baseline recruitment of Herpes simplex virus-specific CD8 T cells expressing CCR10, while interferon-responsive CXCR3 ligands recruit additional cells in response to virus-driven inflammation. PMID: 28701399
    5. We therefore concluded that the cross talk between TNFa and ROS after keratinocytes was exposed to radiation, triggered CCL27 secretion for subsequent inflammation response. PMID: 27879026
    6. Our study showed that a higher immunostaining of CCL27 in supratumoral epidermis is associated with longer progression-free interval and melanoma-specific survival. PMID: 27325798
    7. Our data supports previous reports showing IL-17 and -23 upregulation in association with Multiple sclerosis and potentially identify a previously unknown involvement for CCL27. PMID: 26295034
    8. NOS2 and CCL27: clinical implications for psoriasis and eczema diagnosis and management. PMID: 25539641
    9. Findings support the notion that CCR10 and its ligand CCL27 may contribute to the skin infiltration of malignant T-cells in mycosis fungoides and adult T-cell leukemia/lymphoma. PMID: 24970722
    10. Our study suggests that CTACK/CCL27 may have a pivotal role in the early stage of psoriasis plaque formation, but should be downregulated in the later stage to induce inflammation characteristic for chronic psoriasis plaques PMID: 24710735
    11. Seventeen mediators were identified in burn wound exudates (concentration range 0.0006-9 ng/mg total protein), including the skin-specific chemokine CCL27. PMID: 23980822
    12. We show that the adult but not prenatal human keratinocytes produce and release large amounts of CCL27. PMID: 24037339
    13. CCL27 chemokine implicates cholesteatoma keratinocytes as contributors in cholesteatoma progression. PMID: 23670528
    14. low CCL27/CCR10 and CXCL12/CXCR4 intratumoral mRNA ratios are associated with melanoma progression PMID: 22526457
    15. Serum concentrations of TARC and CTACK were significantly higher in AD (atopic dermatitis) children than in healthy controls, and correlated with severity of symptoms. PMID: 22017510
    16. IL-1beta-induced CCL27 gene expression in normal human keratinocytes is regulated through the p38 MAPK/MSK1/Mnk1+2 as well as the IKKbeta/NF-kappaB signalling pathways. PMID: 21993219
    17. expression of CCL27 and CCL17 in the inflammatory skin diseases: psoriasis, atopic dermatitis and acute allergic contact dermatitis induced in nickel-sensitive individuals PMID: 21707761
    18. member of cytokine family; a chemokine PMID: 11821893
    19. membrane of cytokine family PMID: 11821900
    20. Serum CTACK levels in patients with atopic dermatitis(AD) and psoriasis vulgaris(pSv) were significantly higher. CTACK was strongly expressed in lesional ke-ratinocytes of patients with AD and PsV. PMID: 12642842
    21. serum levels significantly correlated with disease activity in patients with atopic dermatitis PMID: 14767451
    22. primary Th2-dominated inflammatory reaction in atopic dermatitis induced by TARC leads to an augmented skin-specific inflammatory reaction through CTACK. PMID: 15335355
    23. PSGL-1 interacts with CCL27 (CTACK/ILC/ESkine), a skin-associated chemokine that attracts skin-homing T lymphocytes PMID: 15466853
    24. CCL27 expression is under the control of NF-kappaB, and that NF-kappaB, as indicated by others, may be an attractive target for therapy in inflammatory skin diseases PMID: 15598438
    25. IL-1alpha, TNF-alpha, CCL20, CCL27, and CXCL8 alarm signals are induced in human cells after allergen and irritant exposure PMID: 15679580
    26. CCL28 produced by keratinocytes is mediated by different signal pathways from CCL27, and both CCL27 and CCL28 are involved in the pathogenesis of inflammatory skin diseases. PMID: 16433680
    27. CCR10-CTACK/CCL27 interactions between circulating T cells and keratinocytes would seem to play an important role in the pathophysiology of mycosis fungoides. PMID: 16675558
    28. LTB(4) may enhance TNF-alpha-induced CCL27 production by activating NF-kappaB via the BLT1/G(i/o)/PI3K/ERK pathway in human keratinocytes. PMID: 17581202
    29. Human keratinocyte-derived skin tumors may evade T cell-mediated antitumor immune responses by down-regulating the expression of CCL27 through the activation of epidermal growth factor receptor (EGFR)-Ras-MAPK-signaling pathways. PMID: 18025475
    30. Serum concentrations of CCL17, CCL22, and CCL27 correlate well with the extent and intensity of Atopic dermatitis (AD) in infants. Of the three Th2 chemokines examined, serum CCL27 correlated most significantly with the severity of AD PMID: 18266834
    31. a novel mechanism for the recruitment of CCR10-positive T cells to skin-draining LN following the rapid release of preformed CCL27 from the epidermis PMID: 18453562
    32. Significantly lower serum concentration of CXCL-9, CXCL-10, CCL-17, and IL-18 and higher concentration of CXCL-12 and CCL-27 were found in atopic dermatitis patients under 10 years old when compared to Control. PMID: 19639049

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 組織特異性：
    Testis, thymus, placenta, ovary and skin.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 10626

    OMIM: 604833

    KEGG: hsa:10850

    STRING: 9606.ENSP00000259631

    UniGene: Hs.648124