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中文名稱:人胱天蛋白酶9(Casp-9)酶聯(lián)免疫試劑盒
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貨號:CSB-E08862h
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規(guī)格:96T/48T
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價格:¥3200/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Human CASP9 ELISA Kit was designed for the quantitative measurement of Human CASP9 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.
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別名:APAF-3 ELISA Kit; Apoptotic protease Mch-6 ELISA Kit; Apoptotic protease-activating factor 3 ELISA Kit; CASP-9 ELISA Kit; CASP9 ELISA Kit; CASP9_HUMAN ELISA Kit; Caspase 9 ELISA Kit; Caspase-9 subunit p10 ELISA Kit; ICE-LAP6 ELISA Kit; ICE-like apoptotic protease 6 ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates, cell lysates
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檢測范圍:0.78 ng/mL-50 ng/mL
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靈敏度:0.195 ng/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Cell Biology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human Casp-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 90 Range % 84-94 1:2 Average % 96 Range % 91-99 1:4 Average % 91 Range % 87-94 1:8 Average % 100 Range % 94-103 -
回收率:
The recovery of human Casp-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 88-97 EDTA plasma (n=4) 105 97-108 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 50 2.702 2.546 2.624 2.520 25 1.526 1.533 1.530 1.426 12.5 0.745 0.764 0.755 0.651 6.25 0.479 0.486 0.483 0.379 3.12 0.289 0.293 0.291 0.187 1.56 0.212 0.215 0.214 0.110 0.78 0.161 0.164 0.163 0.059 0 0.106 0.102 0.104 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- Comparing the effectiveness of L-carnitine and paraffin oil in acute aluminum phosphide poisoning using predictive biomarkers and scores: A randomized controlled clinical trial WG Abdelhamid,Human & experimental toxicology,2023
- Thiosemicarbazone-metal complexes exhibiting cytotoxicity in colon cancer cell lines through oxidative stress Alcaraz R,Journal of Inorganic Biochemistry,2020
- GABA synaptopathy promotes the elevation of caspases 3 and 9 as pro-apoptotic markers in Egyptian patients with autism spectrum disorder El-Ansary A, et al,Acta Neurologica Belgica,2019
- Antitumor effect and mechanism of action of polysaccharides extracted from Polygonum perfoliatum L whole plant in human lung carcinoma A549 cell line Lai X.et al,Tropical Journal of Pharmaceutical Research,2016
- Zinc chloride inhibits human lens epithelial cell migration and proliferation involved in TGF-β1 and TNF-α signaling pathways in HLE B-3 cells Du Y.et al,Biol Trace Elem Res.,2014
相關(guān)產(chǎn)品
靶點詳情
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功能:Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).; Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
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基因功能參考文獻:
- CASP9 mutations cause recurrent folate-resistant neural tube defects. PMID: 29358613
- Study described rare mutations of the apoptosis gene CASP9 identified in neural tube defect cases and demonstrated that the p.Y251C variant impairs the protein's apoptotic function, suggesting it is a loss-of-function variation; also showed that the p.R191G variant inhibited apoptosis under folate-deficient condition, highlighting the effect of gene-environment interactions in this complex disease. PMID: 29365368
- these results revealed that caspase9 and activated caspase3 predominantly regulates cell apoptosis in human dental pulp stem cells from deciduous teeth. PMID: 29845240
- Low CASP9 expression is associated with Colorectal Cancer. PMID: 29801534
- the results of the present study suggest that miR-96-5p, which is frequently upregulated in hepatocellular carcinoma (HCC), inhibits apoptosis by targeting CASP9. Therefore, miR-96-5p may be a potential therapeutic target for HCC PMID: 29658604
- CASP9 germline mutations may have played a role at least in part to the susceptibility of development of gliomas in a Li-Fraumeni-like family lacking a TP53 germline mutation. PMID: 27935156
- The caspase 9 level was significantly lower and related with oxidant status in patients with polycystic ovary syndrome, while the circulating levels of caspases 3 and 7 were statistically similar in both PCOS and control groups. PMID: 27899026
- Results indicate that the apoptotic protease-activating factor 1 (Apaf-1) apoptosome activates caspase-9 in part through sequestration of the inhibitory caspase recruitment domains (CARDs) domain. PMID: 28143931
- DES1 plays a key role in palmitic acid-mediated caspase 9 and caspase 3 activation. PMID: 27364952
- CASP9 expression associated with inhibition of miR-182. PMID: 28298075
- CASP-9 polymorphism is associated with Primary Brain Tumors. PMID: 28870924
- High CASP9 expression is associated with Lung Tumorigenesis. PMID: 27197231
- knockdown of HMGI-C led to the significant induction of apoptosis via mitochondrial pathway by inducing miR34a and cell cycle arrest in MDA-MB-468 cells in vitro. PMID: 27245202
- Results suggest that the formation of the apoptosome accompanied by the activation of caspase-9 may occur in brains affected by multiple system atrophy (MSA), and that a mitochondria-dependent apoptotic pathway may be partially associated with the pathogenesis of MSA. PMID: 27345387
- Using recombinant proteins, this study investigated the influence of survivin on the inhibition of caspase-9 by XIAP in vitro. With a fluorescence-based assay for the apoptosome-stimulated activity of caspase-9, the study shows that survivin has no effect on the inhibition of caspase-9 by XIAP, neither in the presence nor in the absence of Smac. PMID: 27865841
- Data show that caspase 9 (CASP9) single nucleotide polymorphism (rs1052576) TT genotype was seemed to be associated with higher risk of pathological stage. PMID: 28358701
- The content of caspase 9 gene transcripts in peripheral blood leukocytes and plasma level of TNF-alpha were significantly higher in healthy subjects carrying C allele than in carriers of GG genotype. PMID: 28091912
- Results show that mRNA and protein levels of HAX-1 in prostate cancer cell lines were significantly higher and inhibits cell apoptosis through caspase-9 inactivation. PMID: 26323553
- Renal CASP9 expression is increased in diabetes and increased as diabetic nephropathy progressed. PMID: 27141571
- Inhibition of Caspase-9 restricted, while Apaf-1 promoted, Chlamydia pneumoniae infection in HEp-2, HeLa, and mouse epithelial fibroblast (MEF) cells. PMID: 26290316
- Levels of caspase-9, caspase-10, MAVS, and pIRF7 in mononuclear cells and the disease activity index (SLEDAI) in the systemic lupus erythematosus patients were determined. PMID: 25370148
- Expression of mutant caspase-9 correlated with a downregulation of BAFFR (B-cell-activating factor belonging to the TNF family (BAFF) receptor) in B cells and ICOS (inducible T-cell costimulator) in T cells. PMID: 25569260
- caspase-9 mediates Puma activation to determine the threshold for overcoming chemoresistance in cancer cells. PMID: 25356864
- we suggest that phosphorylation of caspase-9 may be a useful tool to assess the state of gastrointestinal cancer and the effects of anti-cancer therapy. PMID: 25031754
- DNA fragmentation, DNA damage, caspase-9 activation and a large increase in the sub-G1 and S cell cycle phases confirmed the occurrence of apoptosis in a time-dependent manner. PMID: 24377517
- Data indicate a significant association of two single nucleotide polymorphism (SNPs) in aspase9 (CASP9) and two haplotypes of the four SNP combinations with acute myeloid leukemia (AML) susceptibility. PMID: 24879622
- silica and double-stranded RNA (dsRNA) synergistically induces caspase-9-dependent apoptosis, but not inflammasome activation, of bronchial epithelial cells PMID: 24661197
- that KAT5 RNAi may result in cleaved casp9 upregulation through p38MAPK activation in Gallbladder carcinoma cells PMID: 24427328
- overexpression of iASPP and the low expression of caspase-9 in esophageal cancer are closely correlated with tumor invasion and metastasis PMID: 24405603
- the Atg7.caspase-9 complex performs a dual function of linking caspase-9 to the autophagic process while keeping in check its apoptotic activity. PMID: 24362031
- The inhibitory effect of Ab42 on the apoptotic pathway is associated with its interaction with procaspase-9 and consequent inhibition of Apaf-1 apoptosome assembly PMID: 24424093
- The rs4645981 T allele and the rs4645981 T allele carrier might increase the risk of cancer, but the rs1052576 A allele, rs1052576 A carrier, rs2308941 T allele, and rs2308941 T carrier might be protective. PMID: 23479167
- Interactions of caspase-9 with the BIR3 (baculovirus inhibitory repeat 3) exosite are essential for high-affinity binding. PMID: 23203690
- In oligozoospermia, spermatogonia presented significant increased active caspase-9 PMID: 23359247
- The change of caspase-9 expression from colon mucosa, adenoma to cancer suggested it may be involved in the carcinogenesis of colon cancer. PMID: 24592539
- dCas9 effectors can exert positive or negative regulation on the expression of developmentally relevant genes, which can influence cell differentiation status when impinging on a key node in the regulatory network that governs the cell state. PMID: 24346702
- BIRC5-31CC and CASP9+83CT/TT genotypes were associated with an increased risk for renal cell carcinoma development in the female group of our southern European study population. PMID: 23645041
- OSU-03012 induces apoptosis in human esophageal carcinoma cells through a p53/Bax/cytochrome c/caspase-9-dependent pathway. PMID: 23652278
- EGCG, both alone and in combination with cisplatin, promoted the expression of the pro-apoptotic splice isoform of caspase 9 PMID: 23615977
- The results demonstrated that 50 microg/mL beta-glucan significantly repressed the expression of the ERCC5 gene, no change in CASP9 expression, and induction of the CYP1A1 gene. PMID: 23424205
- CASP-9 polymorphisms are associated with susceptibility to low back pain during military training in Chinese soldiers. PMID: 23725396
- Results found that polymorphisms of CASP9 and CASP10 genes may not contribute to CRC risk in Chinese population. PMID: 23303631
- Proteolytic processing of the caspase-9 zymogen is required for apoptosome-mediated activation of caspase-9 PMID: 23572523
- Polymorphism in Caspase 9 (-1263 A>G) was observed to be associated with susceptibility of papillary thyroid carcinoma (PTC). PMID: 22120515
- Induction of caspase-9b expression is due to activation of hnRNP L via phosphorylation to compete/inhibit hnRNP U association with exon 3 of Casp9 mRNA. PMID: 23396972
- These results clearly indicated that olive oil phenolic extract and gallic acid were able to inhibit the caspase 9 dependent apoptosis pathway in HeLa cells. PMID: 22086301
- The overexpression of c-Jun, p73 and Casp-9 in thymic epithelial tumors is closely related with the pathogenesis and biological behavior of the neoplasms. PMID: 22974165
- Data suggest that CD95L-triggered endogenous ceramide increase in Jurkat leukemia T cells is likely involved in the pro-apoptotic mitochondrial pathway leading to caspase-9 activation. PMID: 22306364
- caspase-9 is the most important regulator in DLD-1, HCT-116, and HeLa cells PMID: 23038270
- CASP9 promoter polymorphisms rs4645978 and rs4645981 are associated with breast cancer susceptibility; CASP9 transcriptional regulation is an important factor during breast cancer development PMID: 22981751
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蛋白家族:Peptidase C14A family
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組織特異性:Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
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