This Human CTX-I ELISA Kit was designed for the quantitative measurement of Human CTX-I protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.
別名:
Cross-linked Carboxy-terminal telopeptide of type 1 collagen, CTX-1,
Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I
縮寫:
CTX-I
種屬:
Homo sapiens (Human)
樣本類型:
serum, plasma, tissue homogenates
檢測范圍:
0.625 ng/mL-40 ng/mL
靈敏度:
0.156 ng/mL
反應時間:
1-5h
樣本體積:
50-100ul
檢測波長:
450 nm
研究領域:
Others
測定原理:
quantitative
測定方法:
Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human CTX-I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
88
Range %
83-95
1:2
Average %
95
Range %
91-98
1:4
Average %
97
Range %
90-103
1:8
Average %
96
Range %
91-101
回收率:
The recovery of human CTX-I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
85
81-92
EDTA plasma (n=4)
96
93-106
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Please provide the following information regarding Human Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I ELISA Kit CSB-E11224h a. Is the capture antibody a monoclonal or polyclonal? b. What is the host of the capture antibody? c. Is the detection antibody a monoclonal or polyclonal? d. What is the host of the detection antibody? e. B208. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host? f. If recombinant protein, is it full length or partial? g. What is the species of the standard– human or something else?
A:
Thanks for your inquiry. CSB-E11224h Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody. Standard is synthetic peptide. Pls let me know if you have any further questions. Thank you.