Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Human Cystatin C,Cys-C ELISA Kit

Human Cystatin C,Cys-C ELISA Kit

  • 中文名稱：
    人胱抑素C(Cys-C)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08384h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
        CST3是一種蛋白酶抑制劑，也被稱為卵白素酶抑制劑（SERPINA3），廣泛存在于多種組織和體液中，包括血清、腦脊液、尿液等。CST3參與多種生物學(xué)過程，如細(xì)胞凋亡、細(xì)胞增殖、細(xì)胞遷移和炎癥反應(yīng)等。CST3通過抑制蛋白酶的活性，調(diào)節(jié)細(xì)胞內(nèi)外的蛋白酶活性平衡，從而維持正常的生理狀態(tài)。此外，CST3還與多種疾病的發(fā)生和發(fā)展密切相關(guān)，如阿爾茨海默病、癌癥、糖尿病等。
        華美生物所提供的Human Cystatin C,Cys-C ELISA Kit屬于ELISA檢測(cè)試劑盒，采用雙抗夾心法定量檢測(cè)人血清、血漿、尿液、唾液、組織勻漿樣本中的CST3，其靈敏度為5.824 ng/mL，檢測(cè)范圍為7.8 ng/mL-500 ng/mL。
     
  • 別名：
    AD 8 ELISA Kit; AD8 ELISA Kit; Amyloid angiopathy and cerebral hemorrhage ELISA Kit; ARMD11 ELISA Kit; bA218C14.4 (cystatin C) ELISA Kit; bA218C14.4 ELISA Kit; Cst 3 ELISA Kit; Cst3 ELISA Kit; CST3 protein ELISA Kit; Cystatin 3 ELISA Kit; Cystatin-3 ELISA Kit; Cystatin-C ELISA Kit; Cystatin3 ELISA Kit; CystatinC ELISA Kit; CYTC_HUMAN ELISA Kit; Epididymis secretory protein Li 2 ELISA Kit; Gamma trace ELISA Kit; Gamma-trace ELISA Kit; HCCAA ELISA Kit; HEL S 2 ELISA Kit; MGC117328 ELISA Kit; Neuroendocrine basic polypeptide ELISA Kit; Post gamma globulin ELISA Kit; Post-gamma-globulin ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, urine, saliva, tissue homogenates
  • 檢測(cè)范圍：
    7.8 ng/mL-500 ng/mL
  • 靈敏度：
    5.824 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

     

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human CYSC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

  • 標(biāo)準(zhǔn)曲線：

     

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

     

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

問答及客戶評(píng)論

■ 常見問題解答
Q:

我可以用該試劑盒（CSB-E08384h）檢測(cè)浸泡組織中的胱抑素C嗎？

A:
是浸泡嗎 用什么溶液浸泡的 是甲醛？不過我們確實(shí)沒處理過類似的樣本，而且對(duì)于tissue樣本的均一性差異較大，因此不論什么處理方法，梯度預(yù)實(shí)驗(yàn)是必不可少的；而對(duì)于CSB-E08384h指標(biāo)則要盡可能避免影響蛋白結(jié)構(gòu)的試劑（比如甲醛）。

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    CST3是一種抑制酶類蛋白質(zhì)，也稱為肝素化相關(guān)載體蛋白（HRP），它在細(xì)胞生長(zhǎng)、增殖和分化中起著重要的作用。最新研究表明，CST3參與調(diào)節(jié)免疫系統(tǒng)和腫瘤發(fā)展，對(duì)多種疾病的發(fā)生和發(fā)展具有重要的生物學(xué)功能和臨床價(jià)值，如腫瘤、神經(jīng)系統(tǒng)疾病、心腦血管疾病等。研究者正在通過研究CST3的生物學(xué)功能和臨床應(yīng)用來進(jìn)一步深入了解這個(gè)蛋白質(zhì)的作用機(jī)制和應(yīng)用價(jià)值。
     
  • 功能：
    As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
  • 基因功能參考文獻(xiàn)：
    1. Urinary cystatin-C levels were increased in type 2 diabetic patients with early diabetic nephropathy. PMID: 29673928
    2. Age and cystatin C were found to be significant risk factors for cardiovascular events in a pre-diabetic cohort. PMID: 29661604
    3. Increased cystatin C levels may be associated with enhanced risks of composite endpoints in patients with ST-elevation acute myocardial infarction undergoing elective percutaneous coronary intervention PMID: 29891036
    4. Data suggest that kinetics of degradation/proteolysis versus of aggregation of amyloid beta(1-40) and amyloid beta(1-42) at specific concentrations of amyloid beta, cathepsin B, and cystatin C can be modeled and predicted. PMID: 29046353
    5. Serum CysC, at the time of CRRT (Continuous Renal Replacement Therapy cessation), is an independent predictor of the successful weaning from CRRT in critically ill patients with acute kidney injury. PMID: 29870981
    6. The serum levels of cystatin C and urine NGAL, urine OPN can be used as a good marker for assessing the renal effect of obesity which can lead end stage renal disease in pediatric population. PMID: 30035656
    7. The average of estimated glomerular filtration rate (eGFRave) based on creatinine (eGFRcre) and eGFR based on cystatin C (eGFRcys) best predicted the exacerbation of renal function in allogeneic hematopoietic cell transplantation (allo-HCT). PMID: 29086660
    8. Studied serum Cystatin C as a biomarker in bladder urothelial neoplasm; results found serum Cystatin C not to be a reliable marker in predicting bladder urothelial tumors tumorigenesis and clinicopathological characteristics. PMID: 29789781
    9. bladder cancer (BCa) patients have lower serum cysC concentration than the control group. Serum cysC may be considered as a potential marker of BCa but not its aggressiveness. PMID: 29749360
    10. Postoperative serum Cystatin C, IL-18 and Uric acid after 24h were significantly associated with acute kidney injury PMID: 29627485
    11. Serum CysC is a strong predictor of AKI and the short-term prognosis of CCU patients. PMID: 29179178
    12. Neutrophil gelatinase-associated lipocalin concentrations in the urine and serum on the second day of intensive care unit admission could be used to predict the development of acute kidney injury in the following three to seven days in the intensive care unit; however, the cystatin C concentration did not have predictive value PMID: 28142264
    13. Study demonstrated that the binding of CST3 and PSAP decreased the inhibitory effects of CST3 on proteinase in vitro. The co-localization of both proteins was detected in cultured cells and in Bunina body-containing motor neurons from patients with amyotrophic lateral sclerosis, suggesting that they might be involved in the process of Bunina body formation. PMID: 29249381
    14. elevated cystatin C is independently associated with adverse vascular outcomes in people with suspected or established CAD in terms of all-cause mortality, cardiovascular mortality, and total adverse vascular events [meta-analysis] PMID: 28599259
    15. Cognitive function was significantly and inversely associated with cystatin C and microalbuminuria, in the relatively younger general Japanese population. PMID: 28865325
    16. This paper discusses the current findings regarding the role of Cyst C in cancer progression and the clinical implications emerging from these studies--{review} PMID: 29654808
    17. We enrolled 43 stable coronary artery disease (CAD) male patients to examine the relationship between cystatin C-adiponectin (CysC-APN) complex and coronary plaque characteristics. In multivariate analysis, CysC-APN complex levels were identified as the strongest negative factor for fibrotic components and the strongest positive factor for both necrotic and lipidic plus necrotic components. PMID: 28321013
    18. Urinary cystatin C is an independent risk predictor in patients with acute myocardial infarction. PMID: 28778467
    19. serum Cys-C levels predict renal function in patients with prostate neoplasia, but were not a biomarker for the development of prostate neoplasia, and were not correlated with the clinicopathological characteristics of prostate cancer . PMID: 28812020
    20. There were no associations between serum cysC levels and renal involvement in patients with primary Sjogren's syndrome. PMID: 25367815
    21. cystatin-C should be considered an early biomarker of AKI PMID: 28552392
    22. CysC is as an early acute kidney injury biomarker and predictive of persistent acute kidney injury on aminoglycoside treatment. PMID: 27743042
    23. Cystatin C may be considered a biological marker for vascular dysfunction and subclinical atherosclerosis in SCD. PMID: 27582023
    24. Serum cystatin C level was significantly associated with subclinical atherosclerosis. An increase in cystatin C level could be a valuable surrogate marker for the risk of cardiovascular disease in patients with type 2 diabetes mellitus. PMID: 29090609
    25. Mild-to-moderate kidney dysfunction modifies the relationship between CysC and high-risk coronary lesion, but not NGAL. PMID: 27680774
    26. Increasing cystatin C and cathepsin B reported in serum of colorectal cancer patients. PMID: 28182342
    27. Both cystatin C and beta 2 macroglobulin are elevated in type 2 diabetics with diabetic nephropathy. Cystatin C may be useful in the early diagnosis of diabetic nephropathy PMID: 28182344
    28. Results from nuclear magnetic resonance structural data and molecular dynamics modeling point to conformational requirements and special importance of some amino acid residues in cystatin 3 C-terminal for the its interaction with amyloid beta fragments. PMID: 27714883
    29. The findings of the current studies may be important for future design of hCC dimerization inhibitors. PMID: 27573935
    30. High cystatin C was associated with increased risk of mortality but not with poor functional outcome in patients with acute intracerebral hemorrhage. PMID: 27578255
    31. this results identified cystatin 3 as a biomarker in patients with seasonal allergic rhinitis PMID: 28633877
    32. Serum and urine levels of cystatin C were significantly higher in children with acute kidney injury. [Review and Meta-Analysis] PMID: 28372557
    33. serum level in acute kidney injury patients helps predict risk of chronic kidney disease stage 3 PMID: 27658322
    34. Circulating CysC and Gal-3 were associated with greater left atrial dilatation in patients with heart failure with reduced left ventricular ejection fraction. PMID: 28830298
    35. Application of the cystatin C-based equations most accurately estimate glomerular filtration rate in Chinese patients with chronic kidney disease. PMID: 27125433
    36. This study tested the utility of neutrophil gelatinase-associated lipocalin (NGAL) and cystatin C (CysC) in addition to standard creatinine for early determination of Acute Kidney Injury (AKI) After Heart Operations Using Cardiopulmonary Bypass. The results support the notion that CysC and NGAL should be viewed as comparable for early AKI detection. PMID: 27862029
    37. The strong association between epicardial adipose tissue (EAT) and cystatin C demonstrated herein indicates that EAT accumulation may play an important role in Cystatin C secretion, possibly contributing to cardiometabolic risk in T2DM patients. PMID: 28922364
    38. A high CysC level had predictive value regarding the severity of vascular lesions in acute coronary syndrome. PMID: 28143410
    39. Elevated levels of cystatin C were detected in early diagnosis of right heart failure in patients with systemic sclerosis. PMID: 27414726
    40. biomarker of renal dysfunction, acute-on-chronic liver failure, and hepatorenal syndrome in decompensated liver cirrhosis PMID: 28545169
    41. Data suggest that melanoma cells internalize/absorb cystatin C from culture media, leading to increased intracellular cystatin C levels; cystatin E/M is internalized as well but at modest rate due to down-regulation of cell migration; however, the effect of intracellular cystatin E/M on down-regulation of legumain activity is pronounced. PMID: 28630039
    42. An elevated preoperative Cys-C level was demonstrated to be related with worse survival in patients with renal cell carcinoma(RCC). Measuring preoperative serum Cys-C might be a simple way for finding poor prognostic patients and patients with elevated preoperative Cys-C level should be more closely followed up PMID: 28586363
    43. Acute kidney injury is common following deliberate self-poisoning with a combination washing powder containing oxalic acid and potassium permanganate and cystatin C monitoring may under estimate the severity of poisoning. PMID: 28535124
    44. Cystatin C significantly decreased the lipopolysaccharide-stimulated release and expression of interleukin-1beta and tumor necrosis factor-alpha in monocyte and peripheral blood mononuclear cultures from healthy donors and Crohn's patients. Cystatin C was internalized by monocytes via an active endocytic process, decreased phosphorylation of the MAP kinase pathway ERK-1/2, and altered surface markers. PMID: 27190304
    45. Fibrillation of CysC therefore likely initiates from the monomer and does not require domain-swapping. The non-swapped oligomers likely represent a dead-end offshoot of the amyloid pathway and must dissociate to monomers prior to rearranging to amyloid fibrils. These prefibrillar CysC oligomers were potent inhibitors of aggregation of the Alzheimer's-related peptide, beta-amyloid. PMID: 28487367
    46. CysC plays an important regulatory role in combating cell death via the autophagic pathway in atherosclerosis. PMID: 27079462
    47. Data show that cystatin C deposition in minimally affected samples was limited to the basement membrane (BM) between the dermis and epidermis. PMID: 28067897
    48. Urinary cystatin C levels in the acute kidney injury (AKI) preterm infant group were not significantly higher than in no-AKI group on day of life 1, 3 and 7. PMID: 27020372
    49. Serum cystatin C level was found to be associated with the development and worsening of AKI [Acute kidney injury ] in ICU [intensive care unit ] patients with sepsis. PMID: 28332367
    50. Elevated cystatin C levels at admission were independently associated with impaired myocardial perfusion, poor cardiac functional recovery and development of congestive heart failure in patients with anterior STEMI undergoing PCI. PMID: 26498756

    顯示更多

    收起更多

  • 相關(guān)疾?。?/div>
    Amyloidosis 6 (AMYL6); Macular degeneration, age-related, 11 (ARMD11)
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Cystatin family
  • 組織特異性：
    Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ov
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2475

    OMIM: 105150

    KEGG: hsa:1471

    STRING: 9606.ENSP00000366124

    UniGene: Hs.304682