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  4. Human Dehydroepiandrosterone sulfate,DHEA-S ELISA Kit

Human Dehydroepiandrosterone sulfate,DHEA-S ELISA Kit

  • 中文名稱(chēng)：
    人脫氫表雄酮硫酸酯(DHEA-S)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E05105h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Human DHEA-S ELISA Kit was designed for the quantitative measurement of Human DHEA-S protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 20 ng/mL-4000 ng/mL and the sensitivity is 10 ng/mL.

  • 縮寫(xiě)：
    DHEA-S
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    20 ng/mL-4000 ng/mL
  • 靈敏度：
    10 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human DHEA-S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 90
    Range % 85-103
    1:2 Average % 96
    Range % 89-105
    1:4 Average % 94
    Range % 90-100
    1:8 Average % 90
    Range % 83-110
  • 回收率：
    The recovery of human DHEA-S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 86-103
    EDTA plasma (n=4) 89 84-93
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average
    0 1.953 1.917 1.935
    20 1.321 1.339 1.330
    80 0.843 0.855 0.849
    320 0.511 0.530 0.521
    1000 0.329 0.324 0.327
    4000 0.189 0.184 0.187
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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