Human Diamine acetyltransferase 1(SAT1) ELISA kit
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中文名稱:人二胺乙酰轉(zhuǎn)移酶1(SAT1)酶聯(lián)免疫試劑盒
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貨號:CSB-EL020717HU
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Human SAT1 ELISA Kit was designed for the quantitative measurement of Human SAT1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
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別名:DC21 ELISA Kit; Diamine acetyltransferase 1 ELISA Kit; Diamine N acetyltransferase 1 ELISA Kit; EC 2.3.1.57 ELISA Kit; KFSD ELISA Kit; KFSDX ELISA Kit; Polyamine N acetyltransferase 1 ELISA Kit; Polyamine N-acetyltransferase 1 ELISA Kit; Putrescine acetyltransferase ELISA Kit; SAT ELISA Kit; SAT1 ELISA Kit; SAT1_HUMAN ELISA Kit; Spermidine/spermine N(1) acetyltransferase 1 ELISA Kit; Spermidine/spermine N(1)-acetyltransferase 1 ELISA Kit; Spermidine/spermine N1 acetyltransferase 1 ELISA Kit; Spermidine/spermine N1 acetyltransferase alpha ELISA Kit; SSAT 1 ELISA Kit; SSAT ELISA Kit; SSAT-1 ELISA Kit
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縮寫:SAT1
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:0.156 ng/mL-10 ng/mL
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靈敏度:0.039 ng/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Metabolism
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human SAT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 99 Range % 93-102 1:2 Average % 99 Range % 92-102 1:4 Average % 89 Range % 82-92 1:8 Average % 94 Range % 90-98 -
回收率:
The recovery of human SAT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 88 82-92 EDTA plasma (n=4) 100 93-103 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 10 2.560 2.513 2.537 2.408 5 1.623 1.587 1.605 1.476 2.5 1.094 1.068 1.081 0.952 1.25 0.658 0.648 0.653 0.524 0.625 0.437 0.451 0.444 0.315 0.312 0.273 0.266 0.270 0.141 0.156 0.198 0.188 0.193 0.064 0 0.128 0.130 0.129 -
數(shù)據(jù)處理:
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貨期:3-5 working days
相關(guān)產(chǎn)品
靶點詳情
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功能:Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N(1)- and N(8)-acetylspermidine), spermine, N(1)-acetylspermidine and N(8)-acetylspermidine.
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基因功能參考文獻:
- Activation of SAT1 expression induces lipid peroxidation and sensitizes cells to undergo ferroptosis upon reactive oxygen species (ROS)-induced stress, which also leads to suppression of tumor growth in xenograft tumor models. Notably, SAT1 expression is down-regulated in human tumors, and CRISPR-cas9-mediated knockout of SAT1 expression partially abrogates p53-mediated ferroptosis. Moreover, SAT1 induction is correlated PMID: 27698118
- our results indicated depletion of polyamines by SSAT signi fi cantly inhibited cell proliferation, migration and invasion through AKT/GSK3beta/beta-catenin signaling pathway in hepatocellular carcinoma and colorectal cancer cells. PMID: 27901475
- Extracellular polyamines induced proliferation and cancer cell migration by inducing ODC and SSAT expression, and the Akt1-mediated pathway. PMID: 28157137
- we used siRNA on SSAT and compared the SSAT level in knockdown and normal cells. Results showed that the monoclonal antibody specifically recognized SSAT. PMID: 27328064
- 4H6 was also compared with the commercial antibody. The produced mAbs will be a useful tool for further investigation of SSAT functions in organisms. PMID: 27228136
- Results show low SAT1 brain expression in depressed suicides and implicate low SAT1 brain expression in major depression independent of suicide PMID: 25959060
- Data suggest that SAT1 plays role in apoptosis; overexpression of SAT1 in human embryonic kidney cell line leads to a rapid depletion of spermidine and spermine, arrest in cell growth, and mitochondria-mediated apoptosis. PMID: 25849284
- Analysis shows a significant direct correlation between SSAT expression in Prostatic Cancer tissues and disease progression. PMID: 25893668
- Enhanced SSAT expression by proximal tubule epithelial cells leads to tubular damage, and its deficiency reduces the severity of renal I/R injury through reduction of cellular damage and modulation of the innate immune response PMID: 25390069
- A role for SAT1 in homologous recombination. PMID: 25277523
- The Catabolic enzyme SSAT expression levels was up-regulated in both cell lines; however, the specific SSAT siRNA treatments prevented the EBR-induced apoptosis only in LNCaP (AR+) cells. PMID: 23963538
- In females, the TC genotype was significantly more frequent in alcohol-dependent patients than in non-alcohol-dependent psychiatric controls. No differences were found among the males. PMID: 24735382
- study postulates a mechanism for SAT1 and SMOX down-regulation by post-transcriptional activity of miRNAs. PMID: 24025154
- SAT1 transcription is influenced by lithium and this effect is altered in bipolar disease patients who completed suicide. PMID: 23768751
- berberine inhibits cellular growth by affecting polyamine metabolism, in particular through the upregulation of the key catabolic enzyme, spermidine/spermine N1-acetyltransferase (SSAT). PMID: 23903781
- EBV-positive Akata cells demonstrated decreased SAT1 enzyme activity concomitant with altered intracellular polyamine level. PMID: 23891576
- SSAT translational control mechanisms PMID: 22354986
- SSAT induction plays a role in cell detachment and apoptosis of glioblastoma cells by N1,N11-diethylnorspermine treatment. PMID: 22179681
- The results of this study indicated that epigenetic factors in the promoter region of SAT1 influence gene expression levels, and may provide a mechanism for both our previous findings of haplotype-specific effects of promoter variations on SAT1 expression PMID: 21501848
- Studies indicate that each of the 4 genes was associated with at least one main outcome: anxiety (SAT1, SMS), mood disorders (SAT1, SMOX), and suicide attempts (SAT1, OATL1). PMID: 21152090
- SSAT1 may regulate exogenous gene expression by blocking steps involved in transcription/translation from an episomal vector by targeting non-polyamine substrate(s) critical for this pathway PMID: 20212040
- These results add support for a role of SAT1 in conferring a risk for suicide completion, in particular in the context of depressive disorders. PMID: 19851986
- Knockdown studies suggest that induction of SSAT and SMO is correlated with the antiproliferative effects of BENSpm with 5-FU or paclitaxel in MDA-MB-231 cells. PMID: 19727732
- Induction of alternatively spliced spermidine/spermine N1-acetyltransferase mRNA in the human kidney cells infected by venezuelan equine encephalitis and tick-borne encephalitis viruses PMID: 12083816
- overexpression of SSAT and the consequent putrescine accumulation are involved in the keratosis follicularis spinulosa decalvans phenotype PMID: 12215835
- characterization of promoter function in Hela cells by study of a factor, bound to the responsive element, that underwent modification by binding with another factor after X-ray irradiation PMID: 12427553
- genomic identification and biochemical characterization of an isoenzyme PMID: 12803540
- SSAT has a role in apoptosis induced by sulindac sulfone, which leads to reduced tissue polyamine contents in human colon cancer cells PMID: 14506281
- Transgenic SSAT-overexpressing mice are less active than syngeneic mice and show reduced aggressive behavior; furthermore, SSAT-OE mice have reduced muscle tone and grip strength, although they do not differ from syngeneic mice in several agility tasks. PMID: 15159132
- SSAT has a role in kidney ischemia-reperfusion injury PMID: 15213272
- spermidine acetyltransferase directly binds to the alpha9 cytoplasmic domain and mediates alpha9-dependent enhancement of cell migration PMID: 15479742
- Restoring high inducibility of SSAT activity subverts the reduced sensitivity to cisplatin of SSAT-deficient ovarian cancer cells. PMID: 15905201
- SSAT and SMO(PAOh1) activities are the major mediators of the cellular response of breast tumor cells to polyamines while PAO plays little or no role in this response PMID: 16207710
- Activation of SSAT by aspirin and different NSAIDs may be a common property of NSAIDs that plays an important role in their chemopreventive actions in colorectal cancer. PMID: 16262603
- tertiary structure of hSSAT reported in this article provides a sound basis for the in-depth study of its structure-function relationship PMID: 16544326
- The inhibition of IkappaB and activation of NFkappaB activate SSAT. PMID: 16637064
- a common mediator of inflammation can lead to the induction of SSAT expression by activating the NFkappaB signaling pathway in non-small cell lung cancer cells PMID: 16757480
- These results indicate that the disruption of polyamine homeostasis due to enhanced SSAT activity leads to DNA damage and reduced cell proliferation via activation of DNA repair and cell cycle checkpoint and disruption of Raf --> MEK --> ERK pathways. PMID: 17065202
- The structure of the SSAT-spermine-acetyl-coenzyme A complex suggested that Tyr140 acts as general acid and Glu92, through one or more water molecules, acts as the general base during catalysis. PMID: 17516632
- SSAT1, which shares 46% amino acid identity with SSAT2, also binds to HIF-1alpha and promotes its ubiquitination/degradation. However, in contrast to SSAT2, SSAT1 acts by stabilizing the interaction of HIF-1alpha with RACK1 PMID: 17875644
- Adenovirus-mediated expression of SSAT inhibits colorectal cancer cell growth in vitro. PMID: 18430370
- interaction between SLC3A2 and SAT1 suggests that these proteins may facilitate excretion of acetylated polyamines. PMID: 18660501
- This is the first study linking polymorphic variants of genes involved in polyamine metabolism with anxiety disorders. PMID: 18759322
- Our study provides new results which show that dysregulation of SSAT expression does play a role in suicide behavior. PMID: 19051286
- Downregulation of SAT1 expression may play a role in depression and suicidality. PMID: 19152344
- We failed to demonstrate a significant association between the SAT-1 single nucleotide polymorphism and schizophrenia PMID: 19162121
- results indicate that specific promoter variants in SAT1 have an effect on SAT1 gene expression. PMID: 19446796
- Adenovirus vector-mediated upregulation of spermidine /spermine N1-acetyltransferase impairs human gastric cancer growth in vitro and in vivo. PMID: 19686286
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相關(guān)疾?。?/div>Keratosis follicularis spinulosa decalvans X-linked (KFSDX)亞細胞定位:Cytoplasm, cytosol.蛋白家族:Acetyltransferase family數(shù)據(jù)庫鏈接:
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