Human Estradiol,E2 ELISA Kit
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中文名稱:
人雌二醇(E2)酶聯免疫試劑盒
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貨號:
CSB-E05108h
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規(guī)格:
96T/48T
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價格:
¥3200/¥2500
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其他:
產品詳情
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產品描述:
雌二醇(Estradiol,E2)是一種雌激素,是雌性生殖系統發(fā)育和功能維持的重要激素。E2通過與細胞內的雌激素受體結合,調節(jié)多種生理過程,如生殖器官發(fā)育、月經周期、妊娠、乳腺發(fā)育等。除了生殖系統,E2還參與了神經、心血管、骨骼等系統的生理調節(jié)。E2在體內的水平受到多種因素的影響,如年齡、飲食、運動等。E2水平異常與多種疾病相關,如不孕、乳腺癌、骨質疏松等。E2的生理和病理作用受到廣泛關注,E2也被用于多種藥物的研發(fā)和治療。 華美生物所提供的Human Estradiol,E2 ELISA Kit屬于ELISA檢測試劑盒,采用競爭法定量檢測人血清、血漿樣本中的E2。
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別名:
N/A
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縮寫:
E2
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種屬:
Homo sapiens (Human)
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樣本類型:
serum, plasma.
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檢測范圍:
50 pg/ml-1200 pg/ml.
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靈敏度:
40 pg/ml.
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反應時間:
1-5h
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樣本體積:
50-100ul
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檢測波長:
450 nm
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研究領域:
Signal Transduction
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測定原理:
quantitative
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測定方法:
Competitive
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本試劑盒所含材料:
- A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit E2 antibody.
- Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- Anti-mouse E2 Antibody 1 x 6 ml -- Act as the detection antibody.
- HRP-conjugate 1 x 6 ml -- Bind to the E2 antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
- Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
- Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
- Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
- Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)
- An Instruction manual
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本試劑盒不含材料:
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數據處理:
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貨期:
3-5 working days
引用文獻
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M Wu,Nature aging,2024
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Adipose triglyceride lipase gene expression in peripheral blood mononuclear cells of subjects with obesity and its association with insulin resistance, inflammation and lipid accumulation in liver
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METTL3 drives NSCLC metastasis by enhancing CYP19A1 translation and oestrogen synthesis
W Meng,Cell and Bioscience,2024
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Bisphenol A and its analogue bisphenol S exposure reduce estradiol synthesis via the ROS-mediated PERK/ATF4 signaling pathway
Z Li,Food and chemical toxicology,2023
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MicroRNA Let-7i inhibits granulosa-luteal cell proliferation and estradiol biosynthesis by directly targeting IMP2
X Xu,Reproductive biomedicine online,2023
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Malvidin-3-O-Glucoside Ameliorates Cadmium-Mediated Cell Dysfunction in the Estradiol Generation of Human Granulosa Cells
S Liang,Nutrients,2023
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Iron overload modulates follicular microenvironment via ROS/HIF-1α/FSHR signaling
Y Wu,Free radical biology & medicine,2023
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Fetuin B alleviates testosterone propionate-induced oxidative stress and mitochondrial dysfunction in KGN cells by upregulating the TGFR2/SMAD3 pathway
Y Gao,ResearchSquare,2022
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In vitro and in vivo approaches for identifying the role of aryl hydrocarbon receptor in the development of nonalcoholic fatty liver disease
Xiang-Yu Zhu, et al,Toxicology Letters,2019
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Maria Kupreeva.et al,American Journal of Physiology-Endocrinology and Metabolism,2018
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Prenatal high estradiol exposure induces sex-specific and dietarily reversible insulin resistance through decreased hypothalamic INSR
Wang HH.et al,Endocrinology,2017
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Estrogen is an important mediator of mast cell activation in ovarian endometriomas
Zhu T.et al,Reproduction.,2017
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Highly Sensitive Detection of Hormone Estradiol E2 Using Surface-Enhanced Raman Scattering Based Immunoassays for the Clinical Diagnosis of Precocious Puberty
Rui Wang.et al,ACS applied materials & interfaces,2016
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The effects of EGb761 on lipopolysaccharide-induced depressive-like behaviour in C57BL/6J mice
Yuehan Zhao, Yongdong Zhang, Fang Pan,Centr Eur J Immunol ,2015
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Retinoic acid has the potential to suppress endometriosis development
Yamagata Y. et al,J Ovarian Res,2015
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