Human Galectin-1(LGALS1) ELISA kit
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中文名稱:人乳糖凝集素1(LGALS1)酶聯(lián)免疫試劑盒
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貨號:CSB-EL012882HU
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Human LGALS1 ELISA Kit was designed for the quantitative measurement of Human LGALS1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.
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別名:14 kDa laminin-binding protein ELISA Kit; 14 kDa lectin ELISA Kit; Beta galactoside binding lectin ELISA Kit; Beta galactoside binding lectin L 14 I ELISA Kit; beta galactoside binding protein ELISA Kit; Beta-galactoside-binding lectin L-14-I ELISA Kit; DKFZp686E23103 ELISA Kit; Gal 1 ELISA Kit; Gal-1 ELISA Kit; GAL1 ELISA Kit; Galaptin ELISA Kit; Galbp ELISA Kit; Galectin ELISA Kit; Galectin-1 ELISA Kit; Galectin1 ELISA Kit; GBP ELISA Kit; HBL ELISA Kit; HLBP14 ELISA Kit; HPL ELISA Kit; L 14.5 ELISA Kit; L-14.5 ELISA Kit; L14 ELISA Kit; Lactose binding lectin 1 ELISA Kit; Lactose-binding lectin 1 ELISA Kit; Lect14 ELISA Kit; Lectin galactoside binding soluble 1 ELISA Kit; Lectin galactoside-binding soluble 1 ELISA Kit; LEG1_HUMAN ELISA Kit; LGALS 1 ELISA Kit; LGALS1 ELISA Kit; Lgals1 lectin galactose binding soluble 1 ELISA Kit; MAPK activating protein MP12 ELISA Kit; Putative MAPK activating protein MP12 ELISA Kit; Putative MAPK-activating protein PM12 ELISA Kit; S Lac lectin 1 ELISA Kit; S-Lac lectin 1 ELISA Kit
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縮寫:LGALS1
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates, cell lysates
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檢測范圍:3.12 ng/mL-200 ng/mL
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靈敏度:0.78 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Cell Biology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human LGALS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 86 Range % 80-94 1:2 Average % 85 Range % 80-92 1:4 Average % 92 Range % 87-99 1:8 Average % 97 Range % 90-105 -
回收率:
The recovery of human LGALS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 83-98 EDTA plasma (n=4) 94 88-100 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 200 2.312 2.143 2.228 2.074 100 1.953 1.823 1.888 1.734 50 1.562 1.482 1.522 1.368 25 1.198 1.134 1.166 1.012 12.5 0.647 0.596 0.622 0.468 6.25 0.371 0.347 0.359 0.205 3.12 0.234 0.219 0.227 0.073 0 0.158 0.149 0.154 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Interactive effect of regular aerobic training and milk consumption on some inflammatory markers and lipid profile in overweight boys V TG,Journal of Semnan University of Medical Sciences,/
- Identification of features and differences in PD-1 inhibitor-associated myocarditis and acute myocardial infarction using proteomic analysis Y Luo,/,2024
- Plasma proteomics profiling of PD-1 inhibitor-associated myocarditis and acute myocardial infarction: A clinical and preclinical study Y Luo,Authorea Preprints,2023
- Hypomagnetic field alters circadian rhythm and increases algesia in adult male mice MO Wei-Chuan. et al,Progress in Biochemistry and Biophysics,2015
- Galectin-1 reduction and changes in T regulatory cells may play crucial roles in patients with unexplained recurrent spontaneous abortion Minjun Wu et al,Int J Clin Exp Pathol,2015
- Qualification and Verification of Serological Biomarker Candidates for Lung Adenocarcinoma by Targeted Mass Spectrometry Wu HY. et al,J Proteome Res.,2015
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.
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基因功能參考文獻(xiàn):
- Silencing Gal-1 impaired invasiveness, and decreased S1PR1 expression and overexpression in gastric cancer can promote the expression of S1PR1and invasion of gastric cancer cells. PMID: 30453284
- Association of galectin-1 expression with eosinophilic infiltration of the tumor tissue in stomach and colorectal cancer was detected. PMID: 29926280
- Study shows that recombinant Galectin-1 (Gal-1) could promote the differentiation and invasion of Trophoblast stem cells (TSCs), suggesting that some of Ishikawa cells secretion increase the expression of Gal-1 in TSCs during implantation, which then induced trophoblast differentiation and invasion in vitro. PMID: 28826368
- In conclusion, these findings suggest that the serum levels of Gal-1, Gal-3, and Gal-9 may be associated with large artery atherosclerotic stroke. PMID: 28112232
- High LGALS1 expression is associated with fibrosis in chronic pancreatitis and pancreatic cancer. PMID: 29328490
- Pancreatic stellate cells promote cancer proliferation, migration, and invasion via Gal1-driven pathways. Gene-expression analyses of pancreatic tumor cells exposed to Gal1 reveal modulation of multiple regulatory pathways involved in tumor progression. PMID: 29615514
- Studies indicate tumor-derived galectin-1, galectin-3 and galectin-9 in various cancers and anticancer therapies that target these molecules [Review]. PMID: 29389859
- The expression level of galectin-1 affects survival in patients with glioblastoma multiforme treated with adjuvant radiotherapy PMID: 29378529
- Results show that Galectin-1 teams up with Galectin-3 to induce inflammatory/pro-degradative gene signature in human chondrocytes affecting the progression of osteoarthritis. Also, Galectin-3 was found to induce a pro-degradative-inflammatory gene signature in human chondrocytes, teaming up with Galectin-1 in the pathogenesis of osteoarthritis. PMID: 27982117
- We also found a subset of prostate cancer patient-derived xenografts and prostate cancer patient samples with mild HO-1 and low Gal-1 expression levels. These results highlight a novel function of a human-used drug as a means of boosting the antitumor response PMID: 28512172
- Gal-1, Gal-3 and Gal-9 galectin expression was higher in the myenteric plexus ganglia of chagasic patients PMID: 28554765
- Results have shown that Gal-1 in the farnesyl-bound form acquires the ability to form self-clusters, and the galactoside-binding pocket of Gal-1 in the FTS-bound form plays an important role in self-cluster formation. PMID: 27624845
- Our findings support the introduction of galectin-1 as a reliable diagnostic marker for thyroid carcinomas. Its involvement in cell proliferation, migration, invasion and tumor growth also intimate functional involvement of galectin-1 in the progression of thyroid carcinoma, suggesting its potential as a therapeutic target PMID: 28677745
- This study showed that obese children had significantly higher galectin-1 levels in proportion to fat mass in obese cases than those in healthy children, which may be interpreted as a compensatory increase in an attempt to improve glucose metabolism. PMID: 28728946
- We further demonstrated using the NMR-based hydrogen-deuterium exchange (HDX) that lactose binding increases the exchange rates of residues located on the opposite side of the ligand-binding pocket for hGal1 and hGal8(NTD), indicative of allostery. Additionally, lactose binding induces significant stabilisation of hGal8(CTD) across the entire domain PMID: 28813004
- our findings indicate that galectin-1 plays a pivotal role in the regulation of key processes in cancer cells, such as migration, invasion, and chemoresistance, by modulating FAK and ERK signaling and survivin level. PMID: 28415760
- Gal-3 staining in the nucleus could be a new positive prognosticator for ovarian cancer. PMID: 28594391
- Gal-1 may offer an additional therapeutic target linking anti-angiogenesis and immune checkpoint blockade. PMID: 28473314
- markedly increased brain Gal-1 and S-nitrosylated Gal-1 both in scrapie-infected rodents and human prion diseases. PMID: 27211330
- Gal-1 knockdown dramatically improved drug sensitivity of breast cancer by reducing P-glycoprotein (P-gp) expression via inhibiting the Raf-1/AP-1 pathway, providing a novel therapeutic target to overcome MDR in breast cancer. PMID: 28212576
- the immunosuppressive microenvironment promoted by hepatic stellate cell-derived galectin-1 in hepatocellular carcinoma can be inhibited by miR-22 PMID: 27494859
- Galectin-1 promotes invasion and epithelial mesenchymal transformation in gastric cancer cells via activation of the non-canonical Hh pathway in Gli-1 dependent manner. PMID: 27835885
- Gal-1 level retained independent predictive influence on the risk of developing cGvHD PMID: 27214079
- this study identified 131 Gal-3 and 15 Gal-1 interactors by galectin pulldown experiments combined with quantitative proteomics. PMID: 28576849
- we found that galectin-1 was a novel modulator of MDR1 by proteomic analysis of a model system of leukemia cell lines PMID: 27050374
- Data found Gal-1 to induce EMT and gastric cancer (GC) cell migration and invasion. Also, Gal-1 up-regulated Gli1 expression and beta1 integrin was responsible for Gal-1-induced Gli1 expression and EMT. PMID: 27836001
- Galectin-1-driven production of SDF-1 in pancreatic stellate cells through activation of NF-kappaB promotes metastasis in pancreatic ductal adenocarcinoma. PMID: 28336327
- Gal-1 expression by MM cells was upregulated in hypoxic conditions and stable knockdown of hypoxia inducible factor-1alpha significantly downregulated its expression PMID: 27311934
- Gal1 may be a useful marker for determining whether morphologic changes in oral cells are reactive or neoplastic PMID: 26980012
- The aim of this study was to analyze the syncytium formation abilities of BeWo cells that were gal-1 silenced. PMID: 26418280
- Studies provide evidence that galectin-1 belongs to the group of potential pathogenic molecules in systemic lupus erythematosus. [review] PMID: 28100106
- an intact dimer interface of Gal-1 is required for it to positively regulate H-rasG12V nanoclustering, but negatively K-rasG12V nanoclustering. PMID: 27087647
- Our results suggest that Gal-1 and ASPP2 functionally compete in nanocluster for active Ras on the plasma membrane. ASPP2 dominates the biological outcome, thus switching from a Gal-1 supported growth-promoting setting to a senescence inducing and stemness suppressive program in cancer cells. Our results support Ras nanocluster as major integrators of tumour fate decision events. PMID: 27437940
- Galectin-1 and Galectin-3 are novel-binding partners for human FVIII. Gal-1 binding can influence the procoagulant activity of FVIII. PMID: 27013611
- Proteomics of the interstitial fluid in subcutaneous adipose tissue in vivo identified a novel adipokine, galectin-1, with a potential role in the pathophysiology of type 2 diabetes. PMID: 27282870
- Suggest that galectin-1 is a protective factor against the development of digital vasculopathy in systemic sclerosis. PMID: 24517166
- This study demonstrated that TLR-mediated PI3K activation modulated the invasion and metastasis of ovarian cancer through the production of galectin-1. PMID: 28350104
- Gal-1-regulated carcinoma-associated fibroblast activation promotes breast cancer cell metastasis by upregulating MMP-9 expression. PMID: 27025601
- It is a beta-galactose-binding animal lectin and known to be distributed throughout the body. PMID: 27590897
- Our functional analyses of galectin-1 in urinary bladder urothelial carcinoma provided novel insights into the critical role of galectin-1 in tumor progression and invasion. These results revealed that silencing the galectin-1-mediated MAPK signaling pathway presented a novel strategy for bladder cancer therapy. PMID: 27440446
- the TLR4/Gal-1 signaling pathway regulates lactate-mediated EMT processes through the activation of ADAM10 and ADAM17 in colon cancer cells. PMID: 27837433
- These findings suggest that Gal-1 plays an important role in immune escape of gingival squamous cell carcinoma cells, and Gal-1 expression level may be a useful clinicopathological prognostic marker for this cancer. PMID: 28108653
- Galectin-1 gene silencing would improve the sensitivity of A549/DDP cells to cisplatin in vivo and in vitro. PMID: 27392028
- In this study, gal-1, -2, -3 and -13 were investigated systematically in the trophoblast and decidua compartment of intrauterine growth restriction (IUGR) placentas and normal third trimester control placentas and stratified by fetal gender and gestational age. Gal-1 Expression Shows No Significant Changes in IUGR Placentas in Villous Trophoblasts. PMID: 27070577
- Galectin-1 overexpression activates the FAK/PI3K/AKT pathway by upregulating expression of alphavbeta3 integrin, leading to enhanced hepatocellular carcinoma invasion via epithelial-mesenchymal transition and sorafenib resistance. PMID: 27100895
- results show that HELLP syndrome is associated with increased circulating levels of gal-1 PMID: 26956510
- The integrative analysis of galectins(Gal-1, -3, -4, -9) discriminated IBD from other intestinal inflammatory conditions and could be used as potential mucosal biomarker PMID: 26891020
- Galectin-1 was undetectable in normal and ulcerative colitis colonic epithelium, while galectin-2, galectin-3, and galectin-4 were strongly expressed. PMID: 26885508
- Tumor-driven, unremitting expression of Satb1 in activated Zbtb46+ inflammatory dendritic cells that infiltrate ovarian tumors results in an immunosuppressive phenotype characterized by increased secretion of tumor-promoting Galectin-1 and IL-6. PMID: 26876172
- Epithelial immunostaining for galectin-1 tended to be elevated in fallopian tubes from women with ectopic pregnancy. PMID: 26359845
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亞細(xì)胞定位:Secreted, extracellular space, extracellular matrix. Cytoplasm. Secreted.
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組織特異性:Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamnioti
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