Human Hydroxyacid oxidase 1(HAO1) ELISA kit

說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

This Human HAO1 ELISA Kit was designed for the quantitative measurement of Human HAO1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.69 pg/mL.
別名：

(S) 2 hydroxy acid oxidase ELISA Kit; Glycolate oxidase ELISA Kit; GOX ELISA Kit; GOX1 ELISA Kit; HAO1 ELISA Kit; HAOX1 ELISA Kit; HAOX1_HUMAN ELISA Kit; Hydroxyacid oxidase 1 ELISA Kit; MGC142225 ELISA Kit; MGC142227 ELISA Kit; OTTHUMP00000030231 ELISA Kit
縮寫：

HAO1
Uniprot No.：

Q9UJM8
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

18.75 pg/mL-1200 pg/mL
靈敏度：

4.69 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HAO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	98
	Range %	93-112
1:10	Average %	107
	Range %	103-109
1:20	Average %	93
	Range %	90-97
1:40	Average %	88
	Range %	86-94

回收率：

The recovery of human HAO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-106
EDTA plasma (n=4)	92	90-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
基因功能參考文獻(xiàn)：
1. The study presents the first spectroscopic evidence of the formation of an intermediate with absorbance features resembling those of a flavosemiquinone in the oxidative half-reaction of glycolate oxidase. PMID: 21141873
2. The inhibitor heteroatoms interact with five active-site residues that have been implicated in catalysis in homologous flavodehydrogenases of L-2-hydroxy acids. In addition, the chlorophenyl substituent is surrounded by nonconserved hydrophobic residues. PMID: 20054120
3. These inhibitions suggest that glycolate binds to the active site of the reduced enzyme, and that DCIP also has affinity for the oxidized enzyme. PMID: 17669354
4. Active site and loop 4 movements within human glycolate oxidase. PMID: 18215067
亞細(xì)胞定位：

Peroxisome.
蛋白家族：

FMN-dependent alpha-hydroxy acid dehydrogenase family
組織特異性：

Liver.
數(shù)據(jù)庫鏈接：

HGNC: 4809

OMIM: 605023

KEGG: hsa:54363

STRING: 9606.ENSP00000368066

UniGene: Hs.193640