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  4. Human Neuron-specific enolase,NSE ELISA Kit

Human Neuron-specific enolase,NSE ELISA Kit

  • 中文名稱：
    人神經(jīng)特異性烯醇化酶(NSE)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07961h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
        肌酸磷酸酶B（Enolase 2），是一種酶類蛋白，參與能量代謝和糖酵解，對于細(xì)胞的生存和增殖至關(guān)重要。在某些癌癥中，ENO2的過度表達(dá)與癌細(xì)胞的生長和侵襲能力有關(guān)。
        華美生物所提供的Human Neuron-specific enolase,NSE ELISA Kit屬于ELISA檢測試劑盒，采用雙抗夾心法定量檢測人血清、血漿、組織勻漿樣本中的ENO2，其靈敏度為0.195 ng/mL，檢測范圍為0.78 ng/mL-50 ng/mL。
     
  • 別名：
    2 phospho D glycerate hydrolyase ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Eno 2 ELISA Kit; ENO2 ELISA Kit; ENOG ELISA Kit; ENOG_HUMAN ELISA Kit; Enolase 2 (gamma; neuronal) ELISA Kit; Enolase 2 ELISA Kit; Enolase 2 gamma neuronal ELISA Kit; Enolase2 ELISA Kit; Epididymis secretory protein Li 279 ELISA Kit; Gamma enolase ELISA Kit; Gamma-enolase ELISA Kit; HEL S 279 ELISA Kit; Neural enolase ELISA Kit; Neuron specific enolase ELISA Kit; Neuron specific gamma enolase ELISA Kit; Neuron-specific enolase ELISA Kit; neuronal enriched enolase ELISA Kit; Neurone specific enolase ELISA Kit; NSE ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.78 ng/mL-50 ng/mL
  • 靈敏度：
    0.195 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 84-92  
    1:2 Average % 94  
    Range % 90-98  
    1:4 Average % 99  
    Range % 95-104  
    1:8 Average % 102  
    Range % 96-108  
  • 回收率：
    The recovery of human NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-96  
    EDTA plasma (n=4) 96 90-102  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    50 2.341 2.332 2.337 2.240  
    25 1.981 1.978 1.980 1.883  
    12.5 1.549 1.532 1.541 1.444  
    6.25 1.014 0.993 1.004 0.907  
    3.12 0.658 0.634 0.646 0.549  
    1.56 0.396 0.382 0.389 0.292  
    0.78 0.292 0.288 0.290 0.193  
    0 0.098 0.095 0.097 　  
  • 本試劑盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human NSE antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled NSE antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

靶點詳情

  • 最新研究進展：
    ENO2，又稱為神經(jīng)元特異性烯醇化酶，是一種在神經(jīng)元中廣泛表達(dá)的酶，參與多種神經(jīng)過程的調(diào)節(jié)，包括神經(jīng)元的分化、遷移、突觸形成和記憶等。最新的研究顯示，ENO2的異常表達(dá)與神經(jīng)系統(tǒng)疾病的發(fā)生和發(fā)展密切相關(guān)，例如阿爾茨海默病、帕金森病和多發(fā)性硬化癥等。
  • 功能：
    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • 基因功能參考文獻(xiàn)：
    1. CgA and NSE are clinically valuable tumor markers in neuroblastoma and they merit prospective clinical evaluations as such. PMID: 29737901
    2. the major result was that elevated serum NSE appears to correlate with prognosis in advanced prostate cancer (PCa), particularly in patients with progressive and metastatic castration-resistant PCa. Based on the existing literature, the role of serum NSE in PCa patients should be further evaluated. PMID: 28885659
    3. Identify a possible cross-reactive conformational B cell epitope, shared by Borrelia enolase and gamma enolase, but not by alpha enolase. We hypothesize that this autoantibody interferes with glycolysis in neuronal cells, as the physiological basis for chronic symptoms in at least some cases of post-treatment Lyme disease syndrome. PMID: 29317049
    4. We investigated the possible correlation between TCD results and S100B, NSE and LDH levels as well as between NSE-LDH and S100B-LDH levels by regression analysis. We found a statistically significant relationship for both NSE, S100B with LDH. PMID: 29244749
    5. ENO2 promoted cell growth, glycolysis, and glucocorticoid resistance, all of which were effectively inhibited when ENO2 was silenced with shRNAs and up-regulated various glycolysis-related genes and enhanced Akt activity with subsequent glycogen synthase kinase3beta (GSK-3beta) phosphorylation, inducing cell proliferation and glycolysis. PMID: 29689546
    6. High Neuron-Specific Enolase expression is associated with poor response to therapy in small cell lung cancer. PMID: 28127669
    7. In neonates, NSE and s100B levels increase after bypass surgery and return below preoperative baseline levels by postoperative day seven. PMID: 28571730
    8. elevated preoperative NSE serum level is adverse predictive and prognostic indicator in adenosquamous lung carcinoma PMID: 27623437
    9. There is a significant correlation between mortality in the critically ill patients in the intensive care unit and increased serum concentration of S100B and NSE. PMID: 29222857
    10. The detection of TK1 combined with that of cytokeratin-19 fragment (CYFRA21-1), CEA or NSE increased the diagnostic value of TK1 for lung squamous cell carcinoma, adenocarcinoma and small cell lung cancer, respectively. PMID: 29247745
    11. Serum levels of neuron-specific enolase (and S100B) are both significantly increased postoperatively and predict postoperative cognitive dysfunction. PMID: 27433840
    12. This study indicates that serum neuron-specific enolase is elevated in diabetic ketoacidosis and correlated with the severity of hyperglycemia, ketosis, and acidosis. PMID: 28056586
    13. serum level elevated in small cell lung cancer patients with shorter progression-free survival PMID: 29145241
    14. Levels of NSE and S100B are still elevated 1 year postpartum in women who have had preeclampsia in contrast to women with previous normal pregnancies. PMID: 27653032
    15. High NSE expression is associated with hypoxic-ischemic encephalopathy. PMID: 28931055
    16. we conducted a retrospective study to evaluate the correlation between clinical-pathological parameters, serum levels of neuron-specific enolase and CYFRA21-1, and bone metastases in lung cancer patients.Multivariate logistic regression analysis showed that patients' age concentrations of neuron-specific enolase and histopathological types were the independent risk factors for bone metastases in patients with lung cancer PMID: 28671048
    17. Suggest that NSE may be a practical parameter that can be used to monitor TB activity and treatment response. Elevated serum NSE level originates, at least in part, from macrophages in granulomatous lesions. PMID: 27271274
    18. HER2 and NSE serum levels were independent prognostic factors in patients with brain metastases in breast cancer. Serum HER2 and MMP-9 appear to be associated with BM in metastatic BC patients. Their predictive value for BM still needs to be evaluated in further prospective studies. PMID: 27464303
    19. this study shows that neuron-specific enolase level is both a predictive marker of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors treatment and a prognostic marker in non-small cell lung cancer harboring EGFR mutations patients PMID: 26719155
    20. The study aimed to identify a small set of genetic signatures that may reliably predict the individuals with a high genetic propensity to heroin addiction. A set of 4 genes (JUN, CEBPB, PRKCB, ENO2, or CEBPG) could predict the diagnosis of heroin addiction with the accuracy rate around 85% in our dataset. PMID: 27495086
    21. High neuron-specific enolase expression is associated with poor response to chemotherapy in small cell lung cancer. PMID: 26886220
    22. In pregnant women developing preeclampsia, the levels of NSE remained high throughout pregnancy, compared to healthy women where the levels decline. PMID: 26355117
    23. Serum neuron specific enolase was a useful marker in predicting subclinical brain damage following essential hypertension. PMID: 27000243
    24. BALF and serum NSE, CEA, and CYFRA21.1 elevated in lung cancer, which had prompt value for pathology, especially significant for BALF. PMID: 27072263
    25. Significant levels of CEA, CYFRA 21-1, NSE, and TSGF were detected in the serum. The amounts found were useful for diagnosing non-small cell lung cancer (NSCLC) patients who depended on the currently limited biomarker development. PMID: 27072222
    26. higher serum and nasal secretion levels were measured in patients with chronic rhinosinusitis compared with healthy controls PMID: 26867533
    27. CEA, NSE, CA125 and pro-GRP could serve as biomarkers for SCLC, and CEA and CYFRA21-1 could serve as biomarkers for NSCLC. Pro-GRP, CA125 and CEA were related to the clinical stages of lung cancer PMID: 26560853
    28. Serum neuron-specific enolase level is an independent predictor of overall survival in patients with gastroenteropancreatic neuroendocrine tumors PMID: 26712902
    29. Vimentin, Nestin and WT1. Sox2 was expressed by the stem/progenitor cells of the ventricular zone, whereas the postmitotic neurons of the cortical plate were immunostained by PAX2 and NSE. PMID: 26972711
    30. Elevation of neuron specific enolase and brain iron deposition is associated with beta-propeller protein-associated neurodegeneration. PMID: 26481852
    31. Data show that serum neuron-specific enolase, cytokeratin 19 fragment 21-1, pro-gastrin-releasing peptide, squamous cell carcinoma antigen, tissue inhibitor of metalloproteinase-1, and human epididymis protein 4 are not associated with brain metastases. PMID: 26730601
    32. Neuron specific enolase levels were not effective in predicting outcomes or detecting recurrences of Merkel cell carcinoma. PMID: 26299616
    33. This study inquires into the physiological concentrations of NSE and S100B proteins in CSF, carried out on a sufficiently large group of 601 patients. PMID: 26421286
    34. NSE values is the best predictors of poor 6-month outcome after out-of-hospital cardiac arrest PMID: 26324761
    35. Studied diagnostic Value of Serum Levels of GFAP, pNF-H, and NSE Compared With Clinical Findings in Severity Assessment of Human Traumatic Spinal Cord Injury. PMID: 25341992
    36. The association was studied of intracranial pressure (ICP) and cerebral perfusion pressure (CPP) on S-100B and neuron-specific enolase (NSE) in severe traumatic brain injury (sTBI). PMID: 25518864
    37. In this study, the appearance of NeuN-positive cells were observed in the basal plate as early as 5 month of gestation. PMID: 25904356
    38. the present findings emphasize that S100 and NSE-immunopositivity might be useful for detecting the cause and process of death due to traumatic brain injury. PMID: 25211554
    39. Report ontogenic development of nerve fibers in human fetal livers using immunohistochemical detection of NCAM1/neurone-specific enolase expression. PMID: 25326085
    40. The use of serum NSE as a diagnostic marker for disability progression in multiple sclerosis was not supported. PMID: 25686504
    41. Serum neuron specific enolase levels were found significantly increased in acute stroke cases as compared to controls. PMID: 24838548
    42. Neuron-specific enolase levels were able to diagnose sepsis-associated encephalopathy but with less sensitivity than S100Beta. PMID: 24760429
    43. results suggested the use of serum S-100beta and NSE levels as indicators for brain injury PMID: 26040109
    44. High levels of neuron-specific enolase (NSE), carbohydrate antigen 125 (CA125) and squamous cell carcinoma antigen (SCC) were detected in 306 (63.6%), 89 (18.5%) and 125 (26.0%) non-small cell lung cancer patients, respectively. PMID: 23712355
    45. The role of S100B protein, neuron-specific enolase, and glial fibrillary acidic protein in the evaluation of hypoxic brain injury in acute carbon monoxide poisoning PMID: 24505052
    46. NSE expression is a viable biomarker for MM and can be a useful reference for the design and adjustment of clinical MM treatment programs. PMID: 24796851
    47. Serial NSE analysis during the acute period of ischemic stroke is useful for monitoring hemorrhagic transformation and the blood-brain barrier disruption status PMID: 25183561
    48. Serum NSE levels are closely associated with peripheral neuropathy in patients with diabetes. PMID: 23846809
    49. This is the first study to suggest blood NFL mRNA as a surrogate marker for early prediction of prediabetic peripheral neuropathy, while NSE mRNA levels may be of no diagnostic value in prediabetic patients. PMID: 24733614
    50. In patients treated with mild hypothermia after out of hospital cardiac arrest changes in NSE are more suitable than its absolute serum levels for the prediction of poor neurologic outcome. PMID: 23836424

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  • 亞細(xì)胞定位：
    Cytoplasm. Cell membrane.
  • 蛋白家族：
    Enolase family
  • 組織特異性：
    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 3353

    OMIM: 131360

    KEGG: hsa:2026

    STRING: 9606.ENSP00000229277

    UniGene: Hs.511915