- A 96-well Coated assay plate 1 -- This microplate has been pre-coated with human SARS-CoV-2 S1 RBD antigen.
- Negative Control (1 x 800 μl) -- It is free of the SARS-CoV-2 S1 RBD IgG antibody and used to preclude the false positive.
- Positive Control (1 x 800 μl) -- Used to evaluate the validity, stability, and comparability of the test results.
- HRP-conjugated anti-Human IgG antibody (100 x concentrate) (1 x 120 μl) -- Act as the detection antibody.
- HRP-conjugate Diluent (1 x 20 ml) -- Dilute the HRPconjugated anti-Human IgG antibody.
- Sample Diluent (2 x 20 ml) -- Dilute the sample solution.
- Wash Buffer (25 x concentrate) (1 x 20 ml) -- Wash the unbound regeat.
- TMB Substrate (1 x 10 ml) -- React with HRP, eliciting a chromogenic color reaction.
- Stop Solution (1 x 10 ml) -- Stop the color reaction. The solution turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) -- Seal the microplate when incubation.
- An Instruction manual
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