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Human Pepsinogen II ELISA Kit

  • 中文名稱：
    人胃蛋白酶原II酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E17539h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Human PG II ELISA Kit was designed for the quantitative measurement of Human PG II protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.

  • 縮寫：
    PG II
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates
  • 檢測范圍：
    6.25 ng/mL-400 ng/mL
  • 靈敏度：
    1.56 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human pepsinogen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %102
    Range %96-107
    1:2Average %94
    Range %90-98
    1:4Average %95
    Range %89-99
    1:8Average %93
    Range %87-98
  • 回收率：
    The recovery of human pepsinogen spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9792-103
    EDTA plasma (n=4)8681-92
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    4002.624 2.733 2.679 2.505
    2002.189 2.077 2.133 1.959
    1001.425 1.353 1.389 1.215
    500.746 0.735 0.741 0.567
    250.437 0.425 0.431 0.257
    12.50.312 0.326 0.319 0.145
    6.250.238 0.234 0.236 0.062
    00.175 0.173 0.174
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價