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  4. Human Regenerating islet-derived protein 3-gamma(REG3G) ELISA kit

Human Regenerating islet-derived protein 3-gamma(REG3G) ELISA kit

  • 中文名稱：
    人再生胰島衍生蛋白質(zhì)3-γ(REG3G)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL019549HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Human REG3G ELISA Kit was designed for the quantitative measurement of Human REG3G protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

  • 別名：
    LPPM429 ELISA Kit; Pancreatitis-associated protein 1B ELISA Kit; Pancreatitis-associated protein IB ELISA Kit; PAP IB ELISA Kit; PAP-1B ELISA Kit; PAP1B ELISA Kit; PAPIB ELISA Kit; REG III ELISA Kit; Reg III-gamma ELISA Kit; REG-3-gamma ELISA Kit; REG3G ELISA Kit; REG3G_HUMAN ELISA Kit; Regenerating islet derived 3 gamma ELISA Kit; Regenerating islet-derived protein 3-gamma ELISA Kit; Regenerating islet-derived protein III-gamma ELISA Kit; UNQ429 ELISA Kit
  • 縮寫：
    REG3G
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    3.12 ng/mL-200 ng/mL
  • 靈敏度：
    0.78 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human REG3G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %88-97
    1:2Average %99
    Range %95-107
    1:4Average %104
    Range %100-110
    1:8Average %93
    Range %86-98
  • 回收率：
    The recovery of human REG3G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-99
    EDTA plasma (n=4)9085-94
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2002.519 2.352 2.436 2.296
    1001.923 1.856 1.890 1.750
    501.301 1.254 1.278 1.138
    250.719 0.721 0.720 0.580
    12.50.415 0.387 0.401 0.261
    6.250.324 0.307 0.316 0.176
    3.120.215 0.229 0.222 0.082
    00.142 0.137 0.140
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.
  • 基因功能參考文獻(xiàn)：
    1. This gene encodes a secreted peptide that displays antimicrobial activity against Gram-positive bacteria. PMID: 16931762
    2. a model of CagA-directed REG3gamma expression in gastric epithelial cells via activation of the IL-11/gp130/STAT3 pathway PMID: 22312430
    3. REG III was identified and expressed predominantly in pancreas and testis, but not in small intestine PMID: 15556304
    4. PAP IB represents a good example of duplication and divergence, probably with the acquisition of new functions, thus participating in the evolution of the protein repertoire. PMID: 15777617
  • 亞細(xì)胞定位：
    Secreted. Cytoplasm.
  • 組織特異性：
    Predominantly expressed in pancreas, where it may be restricted to exocrine pancreas. Moderate expression levels in testis and weak in heart, kidney and placenta.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 29595

    OMIM: 609933

    KEGG: hsa:130120

    STRING: 9606.ENSP00000272324

    UniGene: Hs.447084