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Human Thromboxane B2,TX-B2 ELISA Kit

  • 中文名稱:
    人血栓素B2(TXB2)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E08046h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:

    This Human TX-B2 ELISA Kit was designed for the quantitative measurement of Human TX-B2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 9.38 pg/mL-600 pg/mL and the sensitivity is 2.34 pg/mL .

  • 縮寫:
    TX-B2
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍:
    9.38 pg/mL-600 pg/mL
  • 靈敏度:
    2.34 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human TX-B2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 80-105  
    1:2 Average % 95  
    Range % 85-108  
    1:4 Average % 106  
    Range % 90-112  
    1:8 Average % 95  
    Range % 80-110  
  • 回收率:
    The recovery of human TX-B2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 80-110  
    EDTA plasma (n=4) 98 90-108  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    600 3.147 3.121 3.134 2.997  
    300 2.534 2.521 2.528 2.390  
    150 1.750 1.744 1.747 1.610  
    75 1.052 1.046 1.049 0.912  
    37.5 0.659 0.644 0.652 0.514  
    18.75 0.443 0.428 0.436 0.298  
    9.37 0.263 0.284 0.274 0.136  
    0 0.134 0.141 0.138    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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