Human anthrax toxin receptor 1(ANTXR1) ELISA kit
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中文名稱:人炭疽毒素受體1(ANTXR1)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-EL001832HU
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Human ANTXR1 ELISA Kit was designed for the quantitative measurement of Human ANTXR1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.
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別名:Anthrax toxin receptor 1 ELISA Kit; ANTR1_HUMAN ELISA Kit; Antxr1 ELISA Kit; ATR ELISA Kit; Tumor endothelial marker 8 ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:62.5 pg/mL-4000 pg/mL
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靈敏度:15.6 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Neuroscience
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ANTXR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 102 Range % 96-107 1:2 Average % 100 Range % 91-105 1:4 Average % 94 Range % 88-99 1:8 Average % 102 Range % 95-106 -
回收率:
The recovery of human ANTXR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 84-95 EDTA plasma (n=4) 102 95-107 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 4000 1.946 1.988 1.967 1.819 2000 1.489 1.439 1.464 1.316 1000 1.069 1.017 1.043 0.895 500 0.767 0.743 0.755 0.607 250 0.533 0.524 0.529 0.381 125 0.364 0.352 0.358 0.210 62.5 0.289 0.281 0.285 0.137 0 0.151 0.145 0.148 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Serum Proteomic Analysis by Tandem Mass Tag-Based Quantitative Proteomics in Pediatric Obstructive Sleep Apnea Hanrong Cheng,Frontiers in Molecular Biosciences,2022
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靶點(diǎn)詳情
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功能:Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells.; (Microbial infection) Acts as a receptor for protective antigen (PA) of B.anthracis.
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基因功能參考文獻(xiàn):
- Silencing TEM8 may inhibit proliferation of XWLC05 lung cancer cells, promote cell apoptosis, arrest the cell cycle at G1 phase and decrease the migration and invasive ability. PMID: 29115620
- Novel targets ANTXR1 and RSPO2 were confirmed to be suppressed by miR-493 directly. PMID: 28651234
- These studies identify ANTXR1, a class of receptor that is shared by a mammalian virus and a bacterial toxin, as the cellular receptor for Seneca Valley virus. PMID: 28650343
- expression does not affect cytotoxicity to anthrax toxin PMID: 27170489
- Findings suggest that down-regulation of tumor endothelial marker 8 play an important role in the inhibition of tumorigenesis and development of osteosarcoma. PMID: 26996335
- TEM8 may be differentially expressed between wound types and loss of this molecule impacts HaCaT growth and migration, potentially implicating this molecule as a factor involved in successful progression of wound healing. PMID: 26677171
- In the absence of any N-linked glycans, TEM8 fails to fold correctly and is recognized by the ER quality control machinery. PMID: 25781883
- These studies expand the allelic spectrum in this rare condition and potentially provide insight into the role of ANTXR1 in the regulation of the extracellular matrix. PMID: 25045128
- TEM8-targeted siRNAs also offered significant protection against lethal toxin in human macrophage-like cells. PMID: 24742682
- ANTXR2 is expressed by human uterine smooth muscle cell and appears important for normal human uterine smooth muscle cell viability, migration and contractility. PMID: 24060446
- High ANTXR1 accelerates breast tumor growth and lung metastasis. PMID: 23832666
- There is an attenuation of ANTXR1 expression post-infection which may be a protective mechanism that has evolved to prevent reinfection. PMID: 23607659
- Mutations affecting ANTXR1 function are responsible for GAPO syndrome's characteristic generalized defect in extracellular-matrix homeostasis. PMID: 23602711
- Two new splice variants, one encoding a membrane-bound form of the receptor and the other secreted, which we have designated V4 and V5 (the latter being the only variant expressed in the prostate). PMID: 22912819
- An acidic region in the cytosolic tail of ANTXR1 decreases actin association, sending a signal that prevents binding of ANTXR1 to the protective antigen and providing evidence that cytoskeletal dynamics regulate ANTXR1 function. PMID: 22303962
- Disruption of Tem8 results in impaired growth of human tumor xenografts of diverse origin including melanoma, breast, colon, and lung cancer. PMID: 22340594
- The copy number of CEA and TEM-8 mRNA, as detected by a real-time quantitative PCR, appears to be a promising marker for evaluating the risk of tumor spread. PMID: 21573768
- postulate that the developmentally controlled expression of TEM8 modulates endothelial cell response to canonical Wnt signaling to regulate vessel patterning and density PMID: 21829615
- TEM8.1 expression in breast cancer cells confers a more aggressive, proangiogenic phenotype. PMID: 22085271
- TEM8 was expressed at a higher level in the stroma adjacent to the triple-negative breast cancer in all cases, with focal immunoreactive areas within the tumor. PMID: 21965755
- studies reveal that TEM8 exists in different forms at the cell surface, a structure dependent on interactions with components of the actin cytoskeleton PMID: 21129411
- Data show that the two different PA oligomers are equally stabilized by ANTXR interactions. PMID: 21079738
- Results describe the expression, purification and crystallization of human anthrax toxin receptor 1 vWA domain to 1.8 A resolution from a single crystal. PMID: 21206026
- the crystal structure of the TEM8 extracellular vWA domain at 1.7 A resolution. PMID: 20585457
- actin was also found to be essential for efficient heptamerization of anthrax toxin PA, but only when bound to one of its 2 receptors, TEM8 PMID: 20221438
- Here we describe the cloning of the human PA receptor using a genetic complementation approach PMID: 11700562
- This is the first demonstration that the ATR/TEM8 protein is highly expressed in epithelial cells, suggesting that the ATR/TEM8 expression pattern is highly relevant for understanding the pathogenesis of anthrax infection. PMID: 15689409
- results indicate that TEM8 plays a positive role in endothelial cell activities related to angiogenesis PMID: 15777794
- These results suggest that the TEM-8 vW and transmembrane domains may play an important biological role in TEM-8 related tubule formation. PMID: 15993844
- because protective antigen binds to CMG2 with much higher affinity than it does to TEM8, a lower pH is needed to attenuate CMG2 binding to allow pore formation; toxin can form pores at different points in the endocytic pathway PMID: 16141341
- Data show that cells expressing palmitoylation-defective mutant receptors are less sensitive to anthrax toxin due to a lower number of surface receptors as well as premature internalization of protective antigen without a requirement for heptamerization. PMID: 16401723
- TEM8 is a new adhesion molecule linking collagen I or PA to the actin cytoskeleton PMID: 16762926
- TEM8 expression levels in DC-based therapeutic vaccines would allow the selection of a subgroup of patients who are most likely to benefit from therapeutic vaccination. PMID: 19440709
- ANTXR1 does not use an adaptor to bind the cytoskeleton. This peptide orders actin filaments into arrays, demonstrating an actin bundling activity that is novel for a membrane protein PMID: 19817382
- ATR/TEM8 protein is highly expressed in epithelial cells, which represent the primary location for bacterial invasion. PMID: 15689409
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相關(guān)疾病:Hemangioma, capillary infantile (HCI); GAPO syndrome (GAPO)
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亞細(xì)胞定位:Cell membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane; Single-pass type I membrane protein. Cell projection, filopodium membrane; Single-pass type I membrane protein.
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蛋白家族:ATR family
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組織特異性:Detected in umbilical vein endothelial cells (at protein level). Highly expressed in tumor endothelial cells.
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