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Human telomerase,TE ELISA Kit

  • 中文名稱：
    人端粒酶(TE)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08021h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Human telomerase (TE) ELISA Kit was designed for the quantitative measurement of Human telomerase (TE) protein in serum, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 別名：
    N/A
  • 縮寫：
    TE
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, cell culture supernates, urine, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %80-96
    1:2Average %99
    Range %91-105
    1:4Average %94
    Range %92-98
    1:8Average %89
    Range %86-100
  • 回收率：
    The recovery of human TE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8980-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.630 2.501 2.566 2.464
    102.161 2.062 2.112 2.010
    51.602 1.541 1.572 1.470
    2.50.931 0.898 0.915 0.813
    1.250.532 0.501 0.517 0.415
    0.6250.322 0.314 0.318 0.216
    0.3120.241 0.227 0.234 0.132
    00.105 0.099 0.102 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

平均分:
5.0分 - 1 個評價

樣品類型：蛋白（重組/純化） 細胞裂解后的胞漿蛋白

樣品信息：人

稀釋比：其他 1:2

產(chǎn)品評價： 我用CSB-E08021h測量癌細胞裂解液中的端粒酶含量，稀釋一倍，OD在0.3左右。標曲正常，端粒酶定量也符合文獻報道。使用方便，如果可以的話盒子做小一點，更好保存。

By 黃老師

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問答及客戶評論

■ 常見問題解答
Q:

We are interesting in knowing the composition and the origin of the standard as well as capture and biotin antibody in this kit.

A:
Thanks for your inquiry.
Coated antibody: Mouse monoclonal antibody
Detect antibody: Goat polyclonal antibody
Standard: It is prepared with a special method and sorry we cannot provide the detailed information. Hope you could understand.