Mouse CD81 antigen(CD81) ELISA kit
-
中文名稱(chēng):小鼠CD81抗原(CD81)酶聯(lián)免疫試劑盒
-
貨號(hào):CSB-EL004960MO
-
規(guī)格:96T/48T
-
價(jià)格:¥3600/¥2500
-
其他:
產(chǎn)品詳情
-
產(chǎn)品描述:
This Mouse CD81 ELISA Kit was designed for the quantitative measurement of Mouse CD81 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL.
-
別名:Cd81 ELISA Kit; Tapa1CD81 antigen ELISA Kit; 26 kDa cell surface protein TAPA-1 ELISA Kit; Target of the antiproliferative ELISA Kit 1 ELISA Kit; CD antigen CD81 ELISA Kit
-
縮寫(xiě):
-
Uniprot No.:
-
種屬:Mus musculus (Mouse)
-
樣本類(lèi)型:serum, plasma, tissue homogenates, cell lysates
-
檢測(cè)范圍:47 pg/mL-3000 pg/mL
-
靈敏度:11.75 pg/mL
-
反應(yīng)時(shí)間:1-5h
-
樣本體積:50-100ul
-
檢測(cè)波長(zhǎng):450 nm
-
研究領(lǐng)域:Immunology
-
測(cè)定原理:quantitative
-
測(cè)定方法:Sandwich
-
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD81 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 97 Range % 92-101 1:2 Average % 91 Range % 85-96 1:4 Average % 84 Range % 80-88 1:8 Average % 95 Range % 95-99 -
回收率:
The recovery of mouse CD81 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 89-98 EDTA plasma (n=4) 104 100-113 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 3000 2.692 2.600 2.646 2.497 1500 2.057 1.938 1.998 1.849 750 1.376 1.428 1.402 1.253 375 0.909 0.854 0.882 0.733 187.5 0.589 0.609 0.599 0.450 94 0.365 0.371 0.368 0.219 47 0.239 0.247 0.243 0.094 0 0.152 0.145 0.149 -
數(shù)據(jù)處理:
-
貨期:3-5 working days
引用文獻(xiàn)
- Glutamine Supplementation Preserves Glutamatergic Neuronal Activity in the Infralimbic Cortex, Which Delays the Onset of Mild Cognitive Impairment in 3×Tg-AD Female Mice JH Baek,Nutrients,2023
- Plasma endothelial cells-derived extracellular vesicles promote wound healing in diabetes through YAP and the PI3K/Akt/mTOR pathway F Wei,Aging (Albany NY),2020
相關(guān)產(chǎn)品
問(wèn)答及客戶評(píng)論
我想要將我們期望的蛋白質(zhì)與外泌體標(biāo)記物如CD81共定位,以證明該蛋白質(zhì)定位在外泌體膜上。您認(rèn)為我是否可以通過(guò)使用諸如Mouse CD81 antigen(CD81) ELISA kit (code: CSB-EL004960MO)之類(lèi)的試劑盒并用一種感興趣的抗蛋白抗體替代檢測(cè)抗體來(lái)達(dá)到這一目的?我可以將此試劑盒用于細(xì)胞培養(yǎng)基衍生的外泌體或細(xì)胞培養(yǎng)基本身嗎?
靶點(diǎn)詳情
-
功能:Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Essential for trafficking and compartmentalization of CD19 receptor on the cell surface of activated B cells. Upon initial encounter with a microbial pathogen, enables the assembly of CD19-CR2 and B cell receptor complexes at signaling TERMs, lowering the threshold dose of antigen required to trigger B cell clonal expansion and humoral immune response. In T cells, associates with CD4 or CD8 coreceptors and defines the maturation state of antigen-induced synapses with B cells. Facilitates localization of CD3 in these immune synapses, required for costimulation and sustained activation of T cells, preferentially triggering T helper type 2 immune response. Can act both as positive and negative regulator of homotypic or heterotypic cell-cell fusion processes. In myoblasts, associates with another tetraspanin CD9 in complex with PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption. Positively regulates sperm-egg fusion and may be involved in the acrosome reaction. Regulates protein trafficking in intracellular compartments. In T cells, associates with dNTPase SAMHD1 and defines its subcellular location, enabling its degradation by the proteasome and thereby controlling intracellular dNTP levels. Also regulates integrin-dependent migration of macrophages, particularly relevant for inflammatory response in the lung.; (Microbial infection) Specifically required for Plasmodium yoelii infectivity of hepatocytes, controlling sporozoite entry in hepatocytes via the parasitophorous vacuole and subsequent parasite differentiation to exoerythrocytic forms.
-
基因功能參考文獻(xiàn):
- The species-specific traits in CD9 and CD81 distribution during sperm maturation were compared between mice and humans. A mutual position of CD9/CD81 is shown in human spermatozoa in the acrosomal cap, however in mice, CD9 and CD81 occupy a distinct area. PMID: 29671763
- adoptive transfer of wild-type regulatory T cells into CD81-deficient mice was sufficient to promote tumor growth and metastasis; these findings suggested that CD81 modulates adaptive and innate immune responses PMID: 26329536
- CD81-Rac interaction exerts an important regulatory role on the innate and adaptive immunity against bacterial infection and suggests a role for CD81 in the development of novel therapeutic targets during infectious diseases. PMID: 25972472
- Plasmodium yoelii sporozoite rhoptry discharge occurs only in the presence of CD81, providing the first direct evidence for a role of CD81 during sporozoite productive invasion. PMID: 24798694
- In vitro myotubes lacking CD9P-1 or both CD9 and CD81 fuse with a higher frequency than normal myotubes PMID: 23575678
- Data suggest that GPC3 down-regulates hepatocyte proliferation by binding to hedgehog (HH) and down-regulating the HH signaling pathway and binding with CD81, thus making it unavailable to bind to Hhex and causing its nuclear translocation. PMID: 23665349
- CD81 interacts with the T cell receptor to suppress signaling. PMID: 23226274
- CD81 promotes the microvillus formation and/or extension while tetraspanin CD82 inhibits these events. In addition, CD81 enhances the outward bending of the plasma membrane while CD82 inhibits it. PMID: 22079629
- Self-renewing hematopoietic stem cells express CD81 during stress-induced proliferation. PMID: 21931533
- CD81 is required for the formation of actin membrane protrusions via Rac1 activation in adhesion-dependent immune cell migration. PMID: 21677313
- data indicate that CD81 plays a significant role in the final stages of RPE development, controlling cell number and overall developmental pattern PMID: 20882409
- CD81 gene is maternally imprinted, with preferential expression from the paternal allele. PMID: 20579434
- CD81 plays a negative role in B cell activation in vitro and in vivo. PMID: 19737782
- CD81 expression by T cells is critical for their induction of IL-4 synthesis by B cells PMID: 11978781
- Infertility of CD9-deficient mouse eggs is reversed by mouse CD81 PMID: 12086470
- the role of CD81 in the regulation of astrocyte and microglial number, perhaps by regulating cell proliferation by a contact inhibition-dependent mechanism. PMID: 12357429
- Data show that CD81, a putative receptor for hepatitis C virus, is required on hepatocytes for human Plasmodium falciparum and rodent Plasmodium yoelii sporozoite infectivity. PMID: 12483205
- CD81 affects expression of its associated partner CD19 beginning at a B-cell postendoplasmic reticulum compartment where CD81 is necessary for normal trafficking or for surface membrane stability of CD19. PMID: 14530327
- CD81 strengthens multivalent VLA-4 contacts within subsecond integrin occupancy without altering intrinsic adhesive properties to low density ligand. CD81 facilitates both VLA-4-mediated leukocyte rolling and arrest on VCAM-1 PMID: 14532283
- CD81 is required for the association of the B cell antigen receptor and the CD19/CD21 complex with lipid rafts and for enhanced signaling from rafts upon coligation. PMID: 14688345
- CD81 signaling events could be mediated by 14-3-3 adapter proteins, and these signals may be dependent on cellular redox PMID: 14966136
- CD9 and CD81 play complementary roles in sperm-egg fusion PMID: 16380109
- CD81 plays multiple roles in the processing, intracellular trafficking, and membrane functions of CD19. PMID: 16449649
- Murine CD81 does not mediate hepatitis C virus pseudoparticle entry when transfected into human HepG2 tumor cells. PMID: 16641285
- Disruption of the mouse CD81 gene led to a reduction in the fecundity of female mice, and CD81-/- eggs had impaired ability to fuse with sperm. PMID: 17290409
- We conclude that CD81 represents a potential therapeutic target to interfere with leukocyte infiltration and ameliorate inflammatory neurological damage in MS. PMID: 18586096
- tetraspanins CD9 and CD81 regulate cell motility and protease production of macrophages and that their dysfunction may underlie the progression of COPD PMID: 18662991
- CD81 is indispensable for an authentic in vivo hepatitis C virus infection. PMID: 19030166
- CD81 potentially plays a role in macrophage cell line growth regulation. PMID: 19184252
- Inactivation of CD81 by homologous recombination can increase astrocyte and microglial cell proliferation and can lead to anomalously large brain size (30% larger than normal). The effect depends on genetic background. PMID: 12357429
顯示更多
收起更多
-
亞細(xì)胞定位:Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.
-
蛋白家族:Tetraspanin (TM4SF) family
-
組織特異性:Expressed in oocytes (at protein level). Highly expressed in granulosa cells. Expressed in skeletal muscle mainly in endothelial cells of endomysial capillaries, in satellite cells and myoblasts (at protein level). Expressed in hepatocytes (at protein lev
-
數(shù)據(jù)庫(kù)鏈接:
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-