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Mouse Chordin-like protein 1(CHRDL1) ELISA kit

  • 中文名稱(chēng):
    小鼠腱蛋白樣蛋白1(CHRDL1)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EL005378MO
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:

    This Mouse CHRDL1 ELISA Kit was designed for the quantitative measurement of Mouse CHRDL1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 1.95 pg/mL .

  • 別名:
    Chrdl1 ELISA kit; Ng1 ELISA kit; Nrln1Chordin-like protein 1 ELISA kit; Neuralin-1 ELISA kit; Neurogenesin-1 ELISA kit; Ventroptin ELISA kit
  • 縮寫(xiě):
    CHRDL1
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類(lèi)型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    1.95 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Neuroscience
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CHRDL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %92
    Range %88-95
    1:2Average %101
    Range %97-106
    1:4Average %98
    Range %92-103
    1:8Average %86
    Range %82-89
  • 回收率:
    The recovery of mouse CHRDL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-102
    EDTA plasma (n=4)8985-95
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.377 2.356 2.367 2.217
    5001.599 1.687 1.643 1.493
    2500.933 0.997 0.965 0.815
    1250.541 0.568 0.555 0.405
    62.50.355 0.326 0.341 0.191
    31.20.222 0.236 0.229 0.079
    7.80.154 0.167 0.161 0.011
    00.144 0.156 0.150  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. Antagonizes the function of BMP7 and may thus play an important role in the embryonic bone formation. Shows no inhibitory effect on the inducing activity of BMP2. Plays a role during anterior segment eye development.
  • 基因功能參考文獻(xiàn):
    1. CHRDL-1 is a new regulatory target of miR-134 during cortical neuronal differentiation; this is a novel role for miR-134 in the modulation of cortical development. PMID: 21228099
    2. Reperfusion injury of proximal kidney tubules causes a reduction of Chrdl1 expression. PMID: 19357253
  • 亞細(xì)胞定位:
    Secreted.
  • 組織特異性:
    Expressed in heart, brain, lung, liver, kidney and testis.
  • 數(shù)據(jù)庫(kù)鏈接: