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  4. Mouse Collagen Type Ⅱ,Col Ⅱ ELISA Kit

Mouse Collagen Type Ⅱ,Col Ⅱ ELISA Kit

  • 中文名稱：
    小鼠Ⅱ型膠原(Col Ⅱ)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08089m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Mouse Col Ⅱ ELISA Kit was designed for the quantitative measurement of Mouse Col Ⅱ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.87 ng/mL-120 ng/mL and the sensitivity is 0.46 ng/mL.

  • 別名：
    Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
  • 縮寫：
    Col Ⅱ
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.87 ng/mL-120 ng/mL
  • 靈敏度：
    0.46 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 81-92  
    1:2 Average % 101  
    Range % 94-105  
    1:4 Average % 90  
    Range % 85-94  
    1:8 Average % 97  
    Range % 91-100  
  • 回收率：
    The recovery of mouse Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 82-93  
    EDTA plasma (n=4) 104 95-108  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    120 2.668 2.511 2.590 2.401  
    60 2.149 2.100 2.125 1.936  
    30 1.459 1.445 1.452 1.263  
    15 0.858 0.832 0.845 0.656  
    7.5 0.540 0.555 0.548 0.359  
    3.75 0.383 0.389 0.386 0.197  
    1.87 0.292 0.298 0.295 0.106  
    0 0.188 0.190 0.189    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價