Mouse Corticotropin-releasing Factor(CRF) ELISA Kit
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中文名稱:小鼠促腎上腺皮質(zhì)激素釋放因子(CRF)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E14068m
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Mouse CRH ELISA Kit was designed for the quantitative measurement of Mouse CRH protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 2.5 pg/mL-40 pg/mL and the sensitivity is 0.675 pg/mL .
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別名:Crh ELISA kit; Gm1347Corticoliberin ELISA kit; Corticotropin-releasing factor ELISA kit; CRF ELISA kit; Corticotropin-releasing hormone ELISA kit
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縮寫:CRH
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma
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檢測(cè)范圍:2.5 pg/mL-40 pg/mL
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靈敏度:0.675 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Neuroscience
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測(cè)定原理:quantitative
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測(cè)定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CRF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 90-100 1:2 Average % 97 Range % 90-105 1:4 Average % 96 Range % 95-102 1:8 Average % 94 Range % 90-101 -
回收率:
The recovery of mouse CRF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 85-96 EDTA plasma (n=4) 94 87-99 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 40 0.168 0.172 0.170 20 0.345 0.349 0.347 10 0.678 0.675 0.677 5 1.265 1.269 1.267 2.5 2.851 2.860 2.856 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Dysfunctions of the paraventricular hypothalamic nucleus induce hypersomnia in mice 2 QZL Huang,eLife,/
- Hypothalamic CRH Neurons Modulate Sevoflurane Anesthesia and The Post-anesthesia Stress Responses S Jiang,bioRxiv,2023
- High Housing Density-Induced Chronic Stress Diminishes Ovarian Reserve via Granulosa Cell Apoptosis by Angiotensin II Overexpression in Mice J Kim,International journal of molecular sciences,2022
- Dendrobium officinale polysaccharides alleviate depression-like symptoms via regulating gut microbiota-neuroinflammation in perimenopausal mice QP Zhang,Journal of Functional Foods,2021
- Intranasal Oxytocin Administration Ameliorates DSS-induced Abnormal Stress-related Behavior and Intestinal Inflammation in an IBD Mouse Model C Liu,Authorea Preprints,2020
產(chǎn)品評(píng)價(jià)
樣品類型:血清
樣品信息:小鼠
稀釋比:其他 1:5
產(chǎn)品評(píng)價(jià): 使用CSB-E14068m試劑盒檢測(cè)小鼠血清CRF水平,稀釋5倍后正常與模型組及各給藥組均在標(biāo)曲范圍內(nèi),全部實(shí)驗(yàn)在三個(gè)半小時(shí)內(nèi)完成,檢測(cè)結(jié)果較穩(wěn)定,標(biāo)曲良好
By 蘭老師
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Hormone regulating the release of corticotropin from pituitary gland. Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile.
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基因功能參考文獻(xiàn):
- Hypothalamic CRH-expressing neurons orchestrate complex behaviors after stress. PMID: 27306314
- Mice lacking corticotropin-releasing hormone, had an improved ability to escape away from potentially dangerous situations. PMID: 28786031
- our results suggest an inhibitory role of miR-212 on the HPA axis, which acts in a counter-regulatory manner. PMID: 28912247
- Study shows that prolactin was sufficient to suppress corticotrophin-releasing hormone (CRH) mRNA expression in the paraventricular nucleus, however it does not appear to be required for the ongoing regulation of the CRH neurones in lactation. PMID: 28744978
- Study blocked endogenous CRH using 2 chemically distinct antagonists of the principal hippocampal CRH receptor, CRHR1. The antagonists caused a modest reduction of spontaneous excitatory transmission onto CA3 pyramidal cells, mediated. This was accompanied by a decrease in incidence but not amplitude of sharp waves, indicating that CRH synaptic actions are sufficient to alter the output of a complex hippocampal network. PMID: 28460009
- Mice exposed to aggressive confrontations exhibited a similar pattern of species-typical aggressive and non-aggressive behaviors on the first and the last session. Repeated aggressive confrontations promoted an increase in plasma corticosterone. After 10 aggressive confrontation sessions, mice presented a non-significant trend toward reducing hippocampal levels of CRF, which inversely correlated with plasma corticosterone PMID: 28614436
- Data (including data from studies in transgenic mice) suggest that Crh alters Gnrh neuron activity and that estradiol is required for Crh to exert both stimulatory and inhibitory effects on Gnrh neurons. (Crh = corticotropin-releasing hormone; Gnrh = gonadotropin releasing hormone) PMID: 29069304
- The activation of parvalbumin(+) interneurons during morphine withdrawal was crucial for the induction of the negative emotion and the up-regulation of CRH mRNA levels in the central amygdala. PMID: 27385383
- Psychological stress-derived CRF can breach the established endotoxin tolerance in the intestinal mucosa. PMID: 23840363
- CRF plays a marked anxiogenic role at CRF1 receptors in the amygdala of mice exposed to the Elevated plus maze. PMID: 27060334
- GABAA receptor (GABAAR) and the Na(+)-K(+)-2Cl(-) cotransporter (NKCC1), but not the K(+)-Cl(-) cotransporter (KCC2), were expressed in the terminals of the CRH neurons at the median eminence (ME). In contrast, CRH neuronal somata were enriched with KCC2 but not with NKCC1. PMID: 27540587
- Excitability of genetically isolated CRF-receptive (CRFR1) neurons in the central nucleus of the amygdala (CeA) is potently enhanced by CRF and that CRFR1 signaling in the CeA is critical for discriminative fear PMID: 28017470
- activation of the Gq-membrane-associated estrogen receptors rapidly stimulates hypothalamic paraventricular nucleus CRH neurons by suppressing the M-current and potentiating glutamatergic neurotransmission PMID: 27387482
- Data describe a missing function of the stress hormone Crh in the regulation of autophagy activation and present evidence that this effect is linked to maintenance of gut homeostasis under basal conditions. PMID: 26987580
- Real-time PCR analyses revealed that social defeat significantly increased corticotropin-releasing hormone in the paraventricular nucleus. PMID: 25219790
- Data suggest a physiologically relevant role for local corticotropin-releasing hormone signaling towards shaping the neuronal circuitry within the mouse olfactory bulb. PMID: 25224546
- results suggest that long-term, post-natal CRF over-expression increases the rewarding effects of cocaine in individuals with high emotional response to stress. PMID: 25094033
- Knockdown of CRF synthesis in the ventral tegmental area prevented interpeduncular intermediate activation and anxiety during nicotine withdrawal. PMID: 25898242
- selective disruption of Grin1 within central amygdala CRF neurons strongly enhances fear memory PMID: 25340785
- it is shown that CRH and UCN upregulate galectin-1 expression in Ishikawa cell line and macrophages and this effect is mediated through CRHR1. PMID: 25473847
- CRF overexpression throughout the CNS increased startle magnitude and reduced ability to inhibit startle. CRF overexpression confined to inhibitory neurons decreased startle magnitude but had no effect on inhibitory measures. PMID: 25575243
- The stress response neuropeptide CRF increases amyloid-beta production by regulating gamma-secretase activity. PMID: 25964433
- These data suggest critical roles for CRF and CRFR1 in tau-P and aggregation and may have implications for the development of Alzheimer disease cognitive decline. PMID: 25125464
- Study of sex- and genotype-dependent effects of a short, acute predator odor exposure on CRF mRNA levels in stress-related brain regions and subsequently changes in spatial memory retrieval in male and female GAD67 mice and their wildtype littermates PMID: 24946072
- Chronic nicotine exposure upregulates corticotropin releasing factor mRNA in dopaminergic neurons of the posterior ventral tegmental area. PMID: 25402857
- Findings suggest that forebrain-specific overexpression of CRF (CRFOE) limited to development is sufficient to induce enduring alterations in startle plasticity and anxiety, while forebrain CRFOE during adulthood results in a different phenotype profile PMID: 24326400
- Data (including data from studies in knockout mice) suggest that Crh/Crhr1 signal transduction in noradrenergic neurons in cardiac ventricles during morphine withdrawal contributes to cardiovascular dysfunction, mimicking stress-induced dysfunction. PMID: 24490859
- Adiponectin regulates ACTH secretion and the hypothalamic-pituitary-adrenal axis in an AMPK-dependent manner in pituitary corticotroph cells. PMID: 24361598
- Impairment of CBP:CREB interaction reduces CRH expression in the hypothalamus on postnatal day nine, but does not disrupt steady-state CRH expression in adult mice PMID: 23768074
- These observations highlight basic cell-type characteristics of CRH neurons in a mutant mouse. PMID: 23724107
- corticotropin-releasing hormone knockouts are more susceptible to glucocorticoid receptor changes in early phases of stress. PMID: 23430272
- CRH was released during water avoidance stress (WAS) and inhibited NLRP6 expression. WAS induced alterations in the gut microbiota of mice; co-housed nonstressed mice developed enteritis associated with increased CRH and decreased levels of NLRP6. PMID: 23470617
- MicroRNA 375 mediates the signaling pathway of corticotropin-releasing factor (CRF) regulating pro-opiomelanocortin (POMC) expression by targeting mitogen-activated protein kinase 8. PMID: 23430746
- Corticotropin releasing factor expression was induced in the hippocampus of the mouse pilocarpine model of status epilepticus. PMID: 22326386
- Overexpression of CRF in Barrington's nucleus CRF in Barrington's nucleus inhibits bladder function and promotes active coping behavior. PMID: 22882375
- suggesting that perturbations of the CRF system are not the primary cause of decreased cognitive performance PMID: 22336193
- the NMDA receptor is positioned for the postsynaptic regulation of CRF expressing central nucleus of the amygdala neurons PMID: 23063907
- CRH and CRHR1 are dynamic modulators of a variety of signal transduction mechanisms and cellular processes. PMID: 22659651
- corticotropin-releasing factor (CRF), a neuropeptide released in response to acute stressors and other arousing environmental stimuli, acts in the nucleus accumbens of naive mice to increase dopamine release through coactivation of the receptors CRFR1 and CRFR2 PMID: 22992525
- Central CRH hyperdrive on its own or in combination with elevated glucocorticoids is responsible for the increase in anxiety-related behaviour. PMID: 22198557
- CRH plays an important role in stress induced hyperphosphorylation of tau protein. PMID: 22222439
- CRF suppressed GnRH protein levels, Ucn2 can modulate GnRH mRNA levels PMID: 22138165
- Ghrelin activates hypophysiotropic CRF neurons, albeit indirectly. PMID: 22363652
- Short-term central amygdala-corticotropin-releasing factor-overexpression enhanced the stress-induced anxiety-like behaviors in transgenic mice PMID: 21783178
- CRF(1) agonists, Ucn 1 and stressin(1) -A, reduced feeding and induced interoceptive stress, whereas Ucn 2 potently suppressed feeding via a CRF(2) -dependent mechanism without eliciting malaise [stressin 1-A] PMID: 21627635
- CRF signaling in the central nucleus of the amygdala is engaged during binge-like ethanol consumption by C57BL/6J mice. PMID: 22399763
- In mice with MECP2 duplication, reducing the levels of Crh or its receptor, Crhr1, suppressed anxiety-like behavior; in contrast, reducing Oprm1 expression improved abnormal social behavior. PMID: 22231481
- Leucine deprivation stimulates fat loss by increasing expression of corticotrophin-releasing hormone in the hypothalamus via activation of stimulatory G protein/cAMP/protein kinase A/cAMP response element-binding protein pathway. PMID: 21719534
- The immunomodulatory effects of CRH may include an important, albeit not explored yet, role in epidermal tissue remodeling and regeneration and maintenance of tissue homeostasis. PMID: 21765902
- These results show possible differential roles for CRF expressed by distinct loci of the extended amygdala, in mediating stress-induced emotional behaviors. PMID: 20548294
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亞細(xì)胞定位:Secreted.
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蛋白家族:Sauvagine/corticotropin-releasing factor/urotensin I family
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組織特異性:Expressed in parvocellular paraventricular nucleus of the hypothalamus and in medial accessory olivary nucleus.
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