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  4. Mouse GDNF family receptor alpha-like(GFRAL) ELISA kit

Mouse GDNF family receptor alpha-like(GFRAL) ELISA kit

  • 中文名稱：
    小鼠GDNF家族α樣受體(GFRAL)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL009383MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：

    This Mouse GFRAL ELISA Kit was designed for the quantitative measurement of Mouse GFRAL protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

  • 別名：
    Gfral ELISA Kit; Gral ELISA Kit; GDNF family receptor alpha-like ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    25 pg/mL-1600 pg/mL
  • 靈敏度：
    6.25 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GFRAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 92
    Range % 88-98
    1:2 Average % 101
    Range % 97-107
    1:4 Average % 94
    Range % 90-98
    1:8 Average % 97
    Range % 92-101
  • 回收率：
    The recovery of mouse GFRAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 89-100
    EDTA plasma (n=4) 88 84-92
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1600 1.859 1.952 1.906 1.766
    800 1.258 1.260 1.259 1.119
    400 0.720 0.734 0.727 0.587
    200 0.466 0.489 0.478 0.338
    100 0.315 0.328 0.322 0.182
    50 0.229 0.235 0.232 0.092
    25 0.172 0.176 0.174 0.034
    0 0.138 0.141 0.140 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.
  • 基因功能參考文獻：
    1. isolating GFRAL as the receptor for GDF15-induced anorexia and weight loss, the identification of a mechanistic basis for the non-homeostatic regulation of neural circuitry by a peripheral signal associated with tissue damage and stress; findings provide opportunities to develop therapeutic agents for the treatment of disorders with altered energy demand PMID: 28953886
  • 亞細胞定位：
    Cell membrane; Single-pass membrane protein; Extracellular side.
  • 蛋白家族：
    GDNFR family
  • 組織特異性：
    Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius.
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:404194

    UniGene: Mm.213862