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Mouse Insulin-like growth factor-binding protein complex acid labile subunit(IGFALS) ELISA kit

  • 中文名稱:
    小鼠胰島素樣生長(zhǎng)因子結(jié)合蛋白復(fù)合物酸不穩(wěn)定亞基(IGFALS)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EL011094MO
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:

    This Mouse IGFALS ELISA Kit was designed for the quantitative measurement of Mouse IGFALS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

  • 別名:
    Igfals ELISA Kit; Albs ELISA Kit; AlsInsulin-like growth factor-binding protein complex acid labile subunit ELISA Kit; ALS ELISA Kit
  • 縮寫(xiě):
    IGFALS
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    3.12 ng/mL-200 ng/mL
  • 靈敏度:
    0.78 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFALS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %96
    Range %93-99
    1:200Average %84
    Range %80-87
    1:400Average %91
    Range %87-94
    1:800Average %107
    Range %104-110
  • 回收率:
    The recovery of mouse IGFALS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)8581-89
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2001.985 1.901 1.943 1.838
    1001.543 1.578 1.561 1.456
    501.055 1.064 1.060 0.955
    250.657 0.648 0.653 0.548
    12.50.346 0.352 0.349 0.244
    6.250.248 0.268 0.258 0.153
    3.120.191 0.193 0.192 0.087
    00.106 0.104 0.105
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    May have an important role in regulating the access of circulating IGFs to the tissues.
  • 基因功能參考文獻(xiàn):
    1. the Igfals gene has a role in skeletal response of male mice to anabolic hormone therapy PMID: 24424061
    2. A modest reduction in post-natal growth in the null ALS mice and in the ALS-deficient patients was observed PMID: 16114275
  • 亞細(xì)胞定位:
    Secreted, extracellular space.
  • 數(shù)據(jù)庫(kù)鏈接:

    KEGG: mmu:16005

    STRING: 10090.ENSMUSP00000060169

    UniGene: Mm.3135