Mouse Protein FAM132B(FAM132B) ELISA kit
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中文名稱:小鼠FAM132B蛋白(FAM132B)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-EL008059MO
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Mouse FAM132B ELISA Kit was designed for the quantitative measurement of Mouse FAM132B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.68 pg/mL.
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別名:Erfe ELISA Kit; C1qtnf15 ELISA Kit; Ctrp15 ELISA Kit; Fam132bErythroferrone ELISA Kit; Complement C1q tumor necrosis factor-related protein 15 ELISA Kit; Myonectin ELISA Kit
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縮寫:FAM132B
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:18.75 pg/mL-1200 pg/mL
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靈敏度:4.68 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Cell Biology
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FAM132B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 85 Range % 80-90 1:2 Average % 93 Range % 87-99 1:4 Average % 102 Range % 98-107 1:8 Average % 98 Range % 94-102 -
回收率:
The recovery of mouse FAM132B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-101 EDTA plasma (n=4) 89 84-94 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1200 2.388 2.533 2.461 2.297 600 1.560 1.588 1.574 1.410 300 0.978 0.901 0.940 0.776 150 0.622 0.602 0.612 0.448 75 0.363 0.355 0.359 0.195 37.5 0.274 0.295 0.285 0.121 18.75 0.189 0.202 0.196 0.032 0 0.159 0.168 0.164 -
數(shù)據(jù)處理:
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貨期:3-5 working days
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, thereby promoting increased iron absorption and mobilization from stores. Promotes lipid uptake into adipocytes and hepatocytes via transcriptional up-regulation of genes involved in fatty acid uptake.
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基因功能參考文獻(xiàn):
- Erfe(-/-) mice failed to adequately suppress hepcidin expression after infection with Plasmodium berghei K173. Importantly, the sustained production of hepcidin allowed by erythroferrone ablation was associated with decreased parasitemia, providing further evidence that transient iron restriction could be beneficial in the treatment of malaria. PMID: 27658439
- The here presented data unearthed EPO-dependent erythroferrone expression acts as an erythropoiesis-driven regulator of iron metabolism under phenylhydrazine-induced hemolytic anemia. PMID: 27067488
- Erythroferrone contributes to hepcidin suppression and iron overload in a mouse model of beta-thalassemia. PMID: 26276665
- Compared with wild-type mice, Fam132b-deficient mice exhibited a more severe anemia, had higher hepcidin levels and consequently lower serum iron concentration on days 14 and 21, and manifested impaired mobilization of iron from stores (liver and spleen). PMID: 25193872
- myonectin is a novel regulator of cellular autophagy PMID: 24187137
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亞細(xì)胞定位:Secreted.
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蛋白家族:Adipolin/erythroferrone family
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組織特異性:Predominantly expressed in skeletal muscle and, at much lower levels, in other tissues, including lung, eye, smooth muscle, heart, brain and kidney. Within skeletal muscles, higher expression levels in soleus as compared with plantaris. Found in blood (at
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