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  4. Mouse Vinculin(VCL) ELISA kit

Mouse Vinculin(VCL) ELISA kit

  • 中文名稱：
    小鼠黏著斑蛋白(VCL)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL025812MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Mouse VCL ELISA Kit was designed for the quantitative measurement of Mouse VCL protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 28.1 pg/mL-1800 pg/mL and the sensitivity is 7.03 pg/mL.

  • 別名：
    Vcl ELISA Kit; Vinculin ELISA Kit; Metavinculin ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    28.1 pg/mL-1800 pg/mL
  • 靈敏度：
    7.03 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
  • 基因功能參考文獻(xiàn)：
    1. Vinculin is necessary to maintain the integrity of the glomerular filtration barrier by modulating podocyte foot processes and stabilizing intercellular junctions. PMID: 29241625
    2. Vinculin-M94I resulted in 37% reduction in peak sodium current compared to wild type and the combination of Vinculin-M94I and pH 7.0 decreased peak sodium current. PMID: 28218286
    3. Lipid binding increases vinculin activation and turnover at focal adhesions. PMID: 28089450
    4. results suggest that the vinculin-actin interaction participates in FA-mediated mechanotransduction PMID: 28388663
    5. These results suggest that vinculin promotes the nuclear localization of transcription factor TAZ to inhibit the adipocyte differentiation on rigid extracellular matrix. PMID: 28115535
    6. These data demonstrate that Src-mediated phosphorylation is necessary for vinculin activation, and that phosphorylation controls cytoskeletal mechanics by regulating force transmission between the actin cytoskeleton and focal adhesion proteins. PMID: 26240176
    7. results suggest that vinculin promotes directionally persistent cell migration and tension-dependent ECM remodeling in complex 3D environments by increasing cell-ECM adhesion and traction force generation. PMID: 26195589
    8. Force-dependent conformational switch of alpha-catenin controls vinculin binding. PMID: 25077739
    9. Mutant vinculin expressing cells are altered in cell migration, which is accompanied by changes in cell adhesion. PMID: 25358683
    10. show that Src phosphorylation of Y1065 within the C-terminal hairpin regulates Vt-mediated actin bundling and provide a detailed characterization of vinculin Y1065 mutations PMID: 25115937
    11. The E29R mutation might prime the vinculin head for F-actin binding, which results in higher cell stiffness, contractile force, and strengthening of focal adhesions. PMID: 25193699
    12. serine phosphorylation is required for the activation of vinculin and force transmission in focal adhesions. PMID: 24996180
    13. VCL binding talin, but not Arp2/3, is critical for osteoclast function, and its selective inhibition retards physiological bone loss. PMID: 24675074
    14. Crk-associated substrate -vinculin binding significantly affects the dynamics of Crk-associated substrate and vinculin within focal adhesions as well as the size of focal adhesions. PMID: 23974298
    15. This study demonistrated that Deletion of the vinculin disturbs the localization of GFAP in Bergmann glial cells in mice. PMID: 23595642
    16. These results identify vinculin as a major controller of integrin-mediated adhesion dynamics in B cells PMID: 23872053
    17. role for vinculin in ventricular myocyte mechanics and dysfunction PMID: 23561539
    18. Vinculin organizes lamellipodia and lamella F-actin and mediates F-actin retrograde flow engagement at focal adhesion to generate extracellular matrix traction. PMID: 23836933
    19. Data indicate that vinculin is not required for transmission of adhesive and traction forces but is necessary for myosin contractility-dependent adhesion strength and traction force and for the coupling of cell area and traction force. PMID: 23716647
    20. vinculin controls the transmission of intracellular and extracellular mechanical cues that are important for the spatiotemporal assembly, disassembly, and reorganization of focal adhesions to coordinate polarized cell motility PMID: 23375895
    21. alphaE-catenin recruits vinculin to adherens junctions more effectively than alphaN-catenin, partly because of its higher affinity for actin filaments. PMID: 23589308
    22. alpha-Catenin and vinculin cooperate to promote high E-cadherin-based adhesion strength PMID: 23266828
    23. Results indicate a tissue-specific function of vinculin in cartilage whereby it controls chondrocytic differentiation. PMID: 22416133
    24. sca4 activates vinculin and interacts with the actin cytoskeleton; vinculin has roles in Rickettsia pathogenesis PMID: 21841197
    25. These results demonstrate that vinculin's mechano-coupling function is dependent on its conformational state. PMID: 21295550
    26. vinculin is not required for the traditional functions of alpha(IIb) beta(3) or the platelet actin cytoskeleton PMID: 20670372
    27. Data show that mutants of vinculin that are unable to interact with PtdIns(4,5)P, attenuate PIP5K- and LPA-induced neurite retraction. PMID: 20841379
    28. vinculin is an indispensable factor determining HSC repopulation capacity, independent of integrin functions. PMID: 20663867
    29. Vinculin-DeltaEx20 is an active variant supporting adhesion site stabilization without an enhanced mechanical coupling. Its presence in a transgenic animal reveals the potential of splice variants in the vinculin gene to alter vinculin function in vivo. PMID: 20644727
    30. phosphorylation on position Y1065 is essential for accurate incorporation of vinculin into FAs and mechanical behavior of cells. PMID: 20678470
    31. Vinculin facilitates cell invasion into three-dimensional collagen matrices. PMID: 20181946
    32. These results indicate that contractility modulates adhesion strengthening via FAK-dependent, vinculin-containing focal adhesion assembly. PMID: 20205236
    33. The phosphomimic mutations of paxillin can specifically induce the recruitment of vinculin to adhesions independent of myosin II activity. PMID: 20308429
    34. Both the lipid binding and the src-phosphorylation of vinculin's C-terminus are important for cell mechanical behavior. PMID: 20006947
    35. Vinculin found required for control of cell shape, cell mechanics, and rac-dependent lamellipodia formation PMID: 11785963
    36. Integrin-mediated adhesion does not require vinculin to couple with focal adhesion proteins for mechano-chemical signaling in embryonic F9 carcinoma cells. PMID: 11991656
    37. Vinculin regulates survival and motility via ERK by controlling the accessibility of paxillin for FAK interaction. PMID: 15138291
    38. model in which unfurling of the metavinculin tail domain is impaired by the negative charges of the 68-amino acid insert, thus requiring vinculin to fully activate the metavinculin molecule PMID: 15159399
    39. Wild-type vinculin and single tyrosine phosphorylation mutant proteins Y100F and Y1065F were significantly more effective at rescuing the spreading defect of vinculin null cells than the double mutant Y100F/Y1065F. PMID: 15229287
    40. Heterozygous inactivation of the vinculin gene predisposes to stress-induced cardiomyopathy PMID: 15331426
    41. studies indicate that PTEN protein levels are dependent on the maintenance of beta-catenin-MAGI-2 interaction, in which vinculin plays a critical role PMID: 15579911
    42. The Vinculin localized at costameres and intercalated discs. PMID: 15679099
    43. activated vinculin localized at focal adhesions recruits vinexins to focal adhesions PMID: 16126177
    44. The results suggest that vinculin inhibits cell migration by stabilising FAs, and that binding of inositol phospholipids to Vt plays an important role in FA turnover. PMID: 16584805
    45. This is the first report of tissue-specific inactivation of the Vcl gene and shows that it is required for preservation of normal cell-cell and cell-matrix adhesive structures. PMID: 17785437
    46. data demonstrate that vinculin contributes to the mechanical stability under large external forces by regulating contractile stress generation. PMID: 17890382
    47. The expression of vinculin constructs with unmasked binding sites in the head and tail regions induces dramatic focal adhesion growth, which is mediated by their direct interaction with talin. PMID: 18056416
    48. Abl differentially regulates Crk-associated substrate, vinculin, and paxillin in arterial vessels PMID: 19542491

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  • 亞細(xì)胞定位：
    Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, adherens junction. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Vinculin/alpha-catenin family
  • 數(shù)據(jù)庫鏈接：