Mouse interleukin-18 binding prorein(IL-18BP) ELISA Kit
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中文名稱:小鼠白介素18結(jié)合蛋白(IL-18BP)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E17797m
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Mouse IL-18BP ELISA Kit was designed for the quantitative measurement of Mouse IL-18BP protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
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別名:Il18bp ELISA Kit; Igifbp ELISA Kit; Interleukin-18-binding protein ELISA Kit; IL-18BP ELISA Kit; Interferon gamma-inducing factor-binding protein ELISA Kit
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縮寫:IL-18BP
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, cell culture supernates
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檢測(cè)范圍:0.156 ng/mL-10 ng/mL
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靈敏度:0.039 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Immunology
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-18BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 97 Range % 93-100 1:2 Average % 84 Range % 80-88 1:4 Average % 104 Range % 100-107 1:8 Average % 91 Range % 87-95 -
回收率:
The recovery of mouse IL-18BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 100 97-103 EDTA plasma (n=4) 89 84-95 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 10 2.729 2.799 2.764 2.592 5 2.302 2.342 2.322 2.150 2.5 1.513 1.587 1.550 1.378 1.25 0.928 0.965 0.947 0.775 0.625 0.548 0.539 0.544 0.372 0.312 0.381 0.375 0.378 0.206 0.156 0.278 0.274 0.276 0.104 0 0.171 0.173 0.172 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
產(chǎn)品評(píng)價(jià)
樣品類型:組織勻漿液 小鼠結(jié)腸
樣品信息:小鼠
稀釋比:其他 稀釋2倍
產(chǎn)品評(píng)價(jià): 我用了貨號(hào)為CSB-E17797m的Mouse Interleukin-18 Binding Prorein (IL-18BP)ELISA Kit檢測(cè)了DSS誘導(dǎo)結(jié)腸炎之后小鼠結(jié)腸組織勻漿中的IL-18BP蛋白,OD值為2左右,該試劑盒效果不錯(cuò),反應(yīng)靈敏,達(dá)到了預(yù)期實(shí)驗(yàn)的目的
By 胡老師
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.
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基因功能參考文獻(xiàn):
- IL-18BP ameliorates cardiac ischemia/reperfusion injury in part through suppression of Th17 differentiation. PMID: 26340315
- Data show that IL-18bp acts as a sensor for IFN-gamma and can regulate both Th1 and Th17 responses in the CNS. PMID: 20644165
- Administratioan of murine IL-18BP at the time of the booster injection of bovine CII reduces the progressive severity of collagen-induced arthritis; both clinical disease activity and histological scores are decreased by 50%. PMID: 12574381
- functional polarization of Ag-specific CD4(+) Th2 cells entering the CNS during the accelerating phase of experimental autoimmune encephalomyelitis is flexible and dependent on IL-18, which is overturned by IL-18 binding protein PMID: 15778395
- IL-18 and IL-18BP may have roles in testicular development, and also in the regulation of testicular functions under physiological conditions PMID: 18299271
- A single intravenous dose of recombinant mouse IL18BP decreased neointimal hyperplasia and improved arterial L/A ratios in an atherosclerotic balloon-injury animal model. PMID: 18455646
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亞細(xì)胞定位:Secreted.
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數(shù)據(jù)庫(kù)鏈接:
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