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中文名稱:小鼠P物質(zhì)受體(SP-R)酶聯(lián)免疫試劑盒
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貨號:CSB-E08362m
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規(guī)格:96T/48T
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價格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Mouse TACR1 ELISA Kit was designed for the quantitative measurement of Mouse TACR1 protein in serum, plasma, cell lysates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.
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別名:Tacr1; Tac1r; Substance-P receptor; SPR; NK-1 receptor; NK-1R; Tachykinin receptor 1
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, cell lysates, tissue homogenates
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檢測范圍:7.8 pg/mL-500 pg/mL
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靈敏度:1.95 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Others
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 102 Range % 93-106 1:2 Average % 97 Range % 92-99 1:4 Average % 94 Range % 85-98 1:8 Average % 107 Range % 96-110 -
回收率:
The recovery of mouse SP-R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 81-95 EDTA plasma (n=4) 101 92-105 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 500 2.226 2.187 2.207 2.042 250 1.562 1.519 1.541 1.376 125 1.006 1.044 1.025 0.860 62.5 0.661 0.639 0.650 0.485 31.2 0.489 0.523 0.506 0.341 15.6 0.400 0.415 0.408 0.243 7.8 0.274 0.270 0.272 0.107 0 0.168 0.162 0.165 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- UVR-B-induced NKR-1 Expression in Ocular Tissues is blocked by Substance P Receptor Antagonist Fosaprepitant in the Exposed as well as Unexposed Partner Eye. Gross J,Ocul Immunol Inflamm,2020
- Ultraviolet Radiation Exposure of One Eye Stimulates Sympathizing Expression of Neurokinin-1 Receptor but Not Monocyte Chemoattractant Protein-1 in the Partner Eye Gross J, et al,Ophthalmic Research,2019
相關(guān)產(chǎn)品
靶點詳情
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功能:This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin K.
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基因功能參考文獻:
- BoNT/A antinociceptive activity is dependent on integrity of SP/NKA and NK1R. PMID: 28673722
- results indicate the contribution of NK1R signaling in maintaining the homeostasis of the ocular surface under steady-state condition, and that the lack of functional NK1R increases the susceptibility of eyes to develop severe herpes stromal keratitis upon ocular HSV-1 infection PMID: 27798158
- the NK1R-CreER mouse line is a valuable new tool for conditional gene manipulation enabling the visualization and manipulation of cells that express NK1R. PMID: 27712014
- NK1R-/- mice did not exhibit excessive false alarms, compared with wildtypes (response disinhibition) but, as in the 5-Choice ContinuousPerformance Test, they exhibited more perseverative responses throughout the test PMID: 26522842
- NK1R-expressing dorsal horn neurons play a major role in the expression of symptoms of chronic itch. PMID: 25830923
- The results of this study indicated that certain behaviours, disrupted in ADHD, are influenced by an interaction between the BRAS and NK1R, and suggest that ACE inhibitors could provide a novel treatment for this disorder. PMID: 25703442
- the hyperactivity, perseveration and, possibly, inattentiveness of NK1R-/- mice is a direct consequence of a lack of functional NK1R PMID: 25558794
- Arsenic may initiate endothelial dysfunction, resulting in vascular leakage in response to vasoactive agents. PMID: 24448713
- NK1-R expression in the trachea, bronchus, and lung tissue increased in MP-infected BALB/c mice, which may explain why wheezing occurs after MP infection. PMID: 25366726
- The behavioural response of mice lacking NK1r to guanfacine resembles its clinical profile in treatment of ADHD PMID: 25074741
- Together, our findings revealed a critical role of microglial NOX2 in mediating the neuroinflammatory and dopaminergic neurodegenerative effects of SP, which may provide new insights into the pathogenesis of PD. PMID: 25209287
- Both PACAP and SP/NKA, but not NK1 receptors participate in normal motor coordination. PMID: 23499760
- obese-asthma phenotype in mice is accompanied by increased expression of NK1-R on adipose tissues and lung epithelium, reflecting that SP released during inflammation may act directly on these tissues. Blocking NK1-R affects lymphangiogenesis PMID: 23792204
- bone marrow taken from NK-1R deficient mice (Tacr1(-/-)) showed lineage specific B and T cell engraftment deficits compared to wild-type competitor bone marrow cells PMID: 23516556
- Data indicate that NK1R-dendritic cells (DCs) promote cellular immunity that requires IL-12p70. PMID: 23365459
- Data from knockout mice suggest that disruption of NK1R signaling in attention deficit hyperactivity disorder could lead to drug resistance to calcium channel blockers specific for L-type channels. PMID: 22884624
- By activation of the NK1R receptors, it is possible to up-regulate the expression of substance P and NK1R in a protein kinase C, mitogen activated protein kinase, nuclear factor kappa b dependant pathway. PMID: 22040127
- male TACR1 (-/-) mice exhibited blunted approach behavior toward females following the initial interaction compared with C57BL/6 mice. NK-1R signaling may therefore play an important role in pheromone-induced sexual behavior. PMID: 22471406
- This review presented that the NK-1 RECEPTOR knockout mice show a correlation between 5-HT firing rate and PMID: 23089640
- The present studies indicate that alterations in substance P/NK1 receptor system underlie, at least in part, the behavioural and monoaminergic changes in this animal model of depression. PMID: 22155476
- These results indicate that deletion of the NK1 receptor does not alter behavioural susceptibility to chronic repeated stress in mice, but accelerates adaptation of the HPA axis. PMID: 22019828
- assessed the effects of substance P on weight gain in response to a high-fat diet and determined glucose metabolism in Tac1-deficient (Tac1(-/-)) mice PMID: 22009727
- Methamphetamine induces trafficking of striatal neuokinin-1 receptor which is dependent on the activity of dopamine D1 and D2 receptors. PMID: 20730802
- Knockdown of the NK-1R gene in bile duct ligated (BDL) mice induces a decrease in the number of large cholangiocytes (associated with enhanced biliary apoptosis) compared with BDL wild-type mice. PMID: 21596993
- In addition to locomotor hyperactivity, NK1R-/- mice express inattentiveness, perseveration and impulsivity in the 5-choice serial reaction-time task. PMID: 21408181
- results suggest that cigarette smoke produces alveolar macrophages' NK1R overexpression primarily by both promoting neurogenic substance P (SP) release and synergizing NK1R response to neurogenic SP largely via activating NF-kappaB pathway PMID: 20426532
- our proposal that NK1R-/- mice offer a mouse model of ADHD was borne out by our human studies, which suggest that DNA sequence changes in and around the TACR1 gene increase susceptibility to this disorder PMID: 19204064
- Substance P receptor mediated macrophage responses. PMID: 11727773
- substance p-induced bladder inflammation was mast cell dependent and did not require NK1R expression on the mast cell PMID: 12217852
- splenocytes and granuloma cells from neurokinin-1 receptor knockout mice made less interferon-gamma and IgG2a PMID: 12388184
- Loss of NK1 receptors has little effect on chemoreceptor function in the mouse, and thus they play, at best, a minor role in the hypoxic chemoreception process. PMID: 12473075
- Increased respiration after hypoxia was unchanged in NK1-/- neonates but was weaker in adult NK1-/- mice. NK1 receptors aren't needed for prenatal development of the respiratory network or for increased breathing by short-lasting hypoxia in neonates. PMID: 12492418
- increased NK-1 receptor gene expression contributes to the development and maintenance of a hyperalgesic state PMID: 12535787
- Genetic elimination of substance P receptors in mice results in an increased gamma-herpesvirus burden and an altered host response. PMID: 12594288
- Tachykinin NK1 receptor genes are expressed in uterine cells, oocytes, ovaries and embryos from mice, and may play a role in female reproductive function. PMID: 12773411
- protease-activated receptor 2 agonists induced a contraction of murine intestinal smooth muscle that was mediated by nerves and requires both neurokinin 1 and 2 receptors PMID: 12801882
- Restraint stress results in increased dura mater mast cell activation in C57BL mice, but not in neurokinin (NK)-1 receptor knockout mice; stress triggers mast cell activation in dura mater through the activation of NK-1 receptors. PMID: 12867261
- Increased time-dependent expression of the NK1 receptor occurs on bone marrow mast cells after coculture with the cytokines IL-4 and/or stem cell factor and is of functional relevance for the activation of mast cells. PMID: 12902513
- These observations suggest that the amygdala is an important area for the effects of substance P and the NK1 receptor in the motivational properties of opiates, as well as the control of behaviors related to anxiety PMID: 12967989
- NK1 receptors are important in the accurate intensity coding of suprathreshold stimuli. PMID: 14614952
- In an inflammatory model of complete Freund's adjuvant-induced hyperalgesia, substantial reductions in swelling, histological outcome and mechanical hyperalgesia are observed in mice lacking the NK-1 receptor. PMID: 14625445
- Deletion of tachykinin NK1 receptor gene does not alter respiratory network maturation but alters respiratory responses to hypoxia. PMID: 14635705
- Time-limited overexpression of neurokinin-1 receptors in mucosal repair tissue of corpus and antrum. Neurokinin-1 receptors may be involved in gastric wound healing. PMID: 14988833
- peripheral nerve injury increases the expression of substance P (NK-1) receptors in the spinal cord dorsal horn; this is correlated with heat hypersensitivity. PMID: 15042514
- NK-1R and NK-2R have important but differential roles in the regulation of cutaneous inflammatory responses PMID: 15084523
- a significant higher number of corporra lutea in the NK1-R deficient mice in comparison to the wild-type group. PMID: 15236323
- We found NK1 receptors in dopaminergic amacrine cells, indicating that SP neurotransmission may be a universal feature of the circuitry of the dopaminergic amacrine cell. PMID: 15381281
- Effect of receptor expression on mast cell development and their association with mast cells in stomach and bladder tissues. PMID: 15458971
- Activation of NK1 receptors is involved in the maintenance of the inhibitory tone of substantia gelatinosa interneurons. PMID: 15494183
- Separate distributions and the postnatal changes in SP and NK1R suggest the possibility of another nociceptive afferent transmission mechanism, that is, volume transmission, in the Vc other than synapse-mediated transmission. PMID: 15763273
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亞細胞定位:Cell membrane; Multi-pass membrane protein.
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蛋白家族:G-protein coupled receptor 1 family
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