Mouse transforming growth factorβ2,TGFβ2 ELISA Kit
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中文名稱:小鼠轉(zhuǎn)化生長因子β2(TGFβ2)酶聯(lián)免疫試劑盒
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貨號:CSB-E09785m
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規(guī)格:96T/48T
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價格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Mouse TGFB2 ELISA Kit was designed for the quantitative measurement of Mouse TGFB2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .
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別名:Tgfb2 ELISA Kit; Transforming growth factor beta-2 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-2 ELISA Kit; TGF-beta-2)] ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:1.56 pg/mL-100 pg/mL
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靈敏度:0.39 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGFβ2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 97 Range % 92-102 1:200 Average % 91 Range % 85-98 1:400 Average % 90 Range % 88-93 1:800 Average % 100 Range % 96-104 -
回收率:
The recovery of mouse TGFβ2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 87-92 EDTA plasma (n=4) 94 89-100 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 100 2.432 2.552 2.492 2.352 50 2.041 2.076 2.059 1.919 25 1.546 1.604 1.575 1.435 12.5 1.002 1.009 1.006 0.866 6.25 0.509 0.524 0.517 0.377 3.12 0.334 0.359 0.347 0.207 1.56 0.238 0.229 0.234 0.094 0 0.135 0.145 0.140 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Prenatal and Postnatal Exposure to Polystyrene Microplastics Induces Testis Developmental Disorder and Affects Male Fertility in Mice T Zhao,Journal of Hazardous Materials,2022
相關(guān)產(chǎn)品
靶點詳情
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功能:Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.; Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2.; Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.
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基因功能參考文獻(xiàn):
- data suggest a mechanism whereby a stromal hedgehog-TGFbeta2 signaling axis acts to control nephrogenesis. PMID: 29945868
- TGF-beta2 as the crucial mediator of neural precursor cell immunomodulation. PMID: 28945200
- CREBH was identified as a key positive regulator of TGF-beta2 transcription in hepatitis C virus-infected cells. PMID: 28621467
- RUNX1T1 serves as a common angiogenic driver for vaculogenesis and functionality of endothelial lineage cells PMID: 28640846
- APC-derived TSP-1 is essential for the development of an adaptive regulatory immune response induced by TGF-beta2-expressing APCs similar to those located at mucosal and ocular sites. PMID: 26458493
- Epidermal Tgfb2 controls proliferation, differentiation and ECM production by reticular fibroblasts. PMID: 26837596
- Thus, along with TGF-b and MAPK signaling, NFkappaB serves as an important regulatory pathway which following Losartan treatment PMID: 25766329
- Expression of TGFbeta2 was induced in response to elevated canonical Wnt signaling in dystrophic muscles and that the resulting increase in TGFbeta activity affected the behavior of satellite cells in an autocrine or paracrine fashion. PMID: 25520397
- these data shed light on previously unrecognized roles of Mkx in tendinopathy, tenogenesis, and tendon repair as well as in regulating the TGFbeta pathway. PMID: 25332192
- TGFbeta2 regulates hypothalamic TRH expression through TIEG1 during fetal development. PMID: 25448845
- These findings indicate that the lens epithelium of MCT mice has increased expression of TGFb1 and Tgfb2 before cataract affection and that changes in the expression of FGF2 as well as TGFbeta may contribute to the progression of the cataract in the mice. PMID: 24279395
- Endothelial lineage differentiation from induced pluripotent stem cells is regulated by microRNA-21 and transforming growth factor beta2 (TGF-beta2) pathways PMID: 24356956
- work reveals a 'seed and soil' mechanism where TGF-beta2 and TGF-beta-RIII signalling through p38alpha/beta regulates DTC dormancy and defines restrictive (BM) and permissive (lung) microenvironments for HNSCC metastasis PMID: 24161934
- HD3alpha directly interacts with HDAC3 and Akt1 and selectively activates transforming growth factor beta2 (TGFbeta2) secretion and cleavage. PMID: 24045946
- In fetal testis, both cord maintenance and somatic cell development are highly sensitive to the levels of TGFbeta2. PMID: 23303681
- the microenvironment at the verge between inflammation (IL-1beta) and tissue remodeling (TGFbeta2) can strongly promote the process of EndMT. PMID: 22739237
- MKL2 regulates a conserved TGF-beta signaling pathway that is required for angiogenesis and ultimately embryonic survival. PMID: 22899851
- haploinsufficient Tgfb2(+/-) mice have aortic root aneurysm and biochemical evidence of increased canonical and noncanonical TGF-beta signaling PMID: 22772368
- Data indicate that TGFbeta2 promotes valve remodeling and differentiation by inducing matrix organization and suppressing cushion mesenchyme differentiation into cartilage cell lineage during heart development. PMID: 21780244
- These observations identify a novel regulatory axis that includes PPARs, Prdm16, and TGF-beta2 in hematopoiesis. PMID: 21967974
- Laser capture microdissection collected clean populations of primary lip and palate epithelial cells destined to fuse. Transcripts of Bmp-4/Alk-3 and Tgfbeta-2/Alk-5 were restricted to epithelial seam of the fusion site, and epithelium of prefusion site. PMID: 21959449
- beta-catenin causes renal dysplasia via upregulation of Tgfbeta2 and Dkk1. PMID: 21436291
- High TGFbeta 2 is associated with SMO-mediated basal cell carcinoma development. PMID: 20858897
- autocrine stimulation of myeloid progenitors by Tgfbeta2 as one mechanism by which HoxA10 expands this population. PMID: 21087928
- TGFbeta2 activation of MEKK3/ERK1/2/5 signaling modulates Has2 expression and hyaluronan production leading to the induction of epithelial to mesenchymal transformation events. PMID: 20633555
- TGF-beta2 suppressed macrophage cytokine production in the developing intestine and protected against inflammatory mucosal injury. PMID: 20875417
- Gene expression analysis shows that transcripts of tgfbeta2 and tgfbeta3 but not tgfbeta1 are abundant in the growing undifferentiated limb mesoderm. PMID: 20565961
- Reduction of TGFbeta2 gene dosage in the RA receptor-deficient background restores septation but does not rescue alignment defects, indicating that excess TGFbeta causes septation defects. PMID: 20230754
- Data show that TNF-alpha induces the formation of fibrotic foci by cultured retinal pigment epithelial cells through activation of transforming growth factor-TGF-beta signaling in a manner dependent on hyaluronan-CD44-moesin interaction. PMID: 19965872
- the perichondrium is essential for the effects of Shh on chondrocyte differentiation but that TGFbeta2 specifically is required for this effect. TGFbeta2 is required for Shh-mediated of PTHrP expression. PMID: 11934857
- TGFbeta2 plays a role in apoptosis in interdigital webs of the developing mouse limb PMID: 11960699
- TGFbeta2 is expressed and obligatory for mammalian endocardial cushion cell transformation PMID: 12142029
- Altered renin synthesis and secretion in the kidneys of heterozygous mice with a null mutation in the TGF-beta(2) gene. Renal renin mRNA and protein levels were higher in mutant mice under hydrated or dehydrated conditions. PMID: 12381922
- results show that TGF-betas obviously play a fundamental role in midline fusion and the TGFbeta2 gene seems to play a more important role in mediating developmental processes than the TGFbeta3 gene PMID: 12478370
- TGFb2 is regulated by the type III transforming growth factor beta receptor PMID: 12773577
- Tgfbeta2 has roles in myocardialisation and in epicardial development PMID: 12815630
- TGF-beta2 plays essential roles in cornea and lens development PMID: 12838410
- TGF-beta2 is a genetically determined positive regulator of primitive hematopoietic progenitor cells PMID: 14707111
- The TGF-beta2 suggested to play a role in the regulation of chromaffin cells proliferation and differentiation based on in vitro experiments. PMID: 15478122
- A high degree of TGF-ss2-specific SMAD2 signaling goes concomitant with vascular innervation of the fourth arch artery. PMID: 15528466
- TGF beta 2 contributes to the development of the cochleovestibular ganglion in mouse embryos PMID: 15744664
- HDL subfraction 3 selectively induces TGF-beta2 mRNA expression and protein release PMID: 15911702
- The highly restrictive and spatial and temporal pattern of expression of Tgf-beta2 implicates its role in mediating the fusion of developing midfacial processes, possibly through interacting with bone morphogenetic protein 4 (Bmp4) PMID: 16026545
- The results clearly demonstrate that TGF-beta2 and TGF-beta3 are essential signals for differentiation of midbrain progenitors toward neuronal fate and dopaminergic phenotype. PMID: 16741229
- Pax3 regulation of TGFbeta2 may play a pivotal role during early embryonic development PMID: 16787918
- We conclude that striatal TGF-beta may be essential for permitting exogenous GDNF to act as a neuroprotective factor. PMID: 17141511
- Zac1 employs a novel cell non-autonomous strategy to regulate amacrine cell number, acting in cooperation with a second tumor suppressor gene, TGFbetaII, through a negative feedback pathway. PMID: 17559664
- TGFbeta2 pathway is critical in regulating morphogenesis in response to noncanonical Wnt signaling. PMID: 17767158
- Findings suggest that augmented nephron number in TGF-beta2(+/-) kidneys results from accelerated ureteric branching morphogenesis and nephron formation. PMID: 18317401
- Data show that TGF-beta2 influences synaptic function, rather than synaptogenesis, specifically at central synapses. PMID: 18854036
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亞細(xì)胞定位:[Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-2]: Secreted.
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蛋白家族:TGF-beta family
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