Mouse trypsin ELISA Kit
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中文名稱:小鼠胰蛋白酶(trypsin)酶聯(lián)免疫試劑盒
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貨號:CSB-E09900m
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規(guī)格:96T/48T
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價(jià)格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Mouse trypsin ELISA Kit was designed for the quantitative measurement of Mouse trypsin protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL .
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縮寫:trypsin
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍:1.56 ng/mL-100 ng/mL
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靈敏度:0.39 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 89-95 1:2 Average % 103 Range % 99-107 1:4 Average % 92 Range % 87-96 1:8 Average % 98 Range % 94-101 -
回收率:
The recovery of mouse trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 87-96 EDTA plasma (n=4) 95 89-98 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 100 2.219 2.112 2.166 2.010 50 1.624 1.576 1.600 1.444 25 1.001 0.991 0.996 0.840 12.5 0.679 0.666 0.673 0.517 6.25 0.377 0.356 0.367 0.211 3.12 0.304 0.313 0.309 0.153 1.56 0.260 0.273 0.267 0.111 0 0.155 0.157 0.156 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Antitumor Activity of Tenacissoside H on Esophageal Cancer through Arresting Cell Cycle and Regulating PI3K/Akt-NF-κB Transduction Cascade. Jia YS.et al,Evid Based Complement Alternat Med.,2015
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