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  4. Pig Interferon α,IFN-α ELISA Kit

Pig Interferon α,IFN-α ELISA Kit

  • 中文名稱：
    豬α干擾素(IFN-α)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07328p
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Pig IFNA1 ELISA Kit was designed for the quantitative measurement of Pig IFNA1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .

  • 縮寫：
    IFN-α
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %80-99
    1:2Average %98
    Range %91-105
    1:4Average %96
    Range %92-105
    1:8Average %94
    Range %86-98
  • 回收率：
    The recovery of pig IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-102
    EDTA plasma (n=4)9790-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.895 2.852 2.874 2.739
    2502.114 2.117 2.116 1.981
    1251.254 1.260 1.257 1.122
    62.50.846 0.839 0.843 0.708
    31.20.489 0.454 0.472 0.337
    15.60.301 0.319 0.310 0.175
    7.80.257 0.249 0.253 0.118
    00.133 0.137 0.135 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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