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  4. Pig glucagon like peptide 1(GLP1) ELISA Kit

Pig glucagon like peptide 1(GLP1) ELISA Kit

  • 中文名稱：
    豬胰高血糖素樣肽1(GLP1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EQ027281PI
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Pig GLP1 ELISA Kit was designed for the quantitative measurement of Pig GLP1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 縮寫：
    GLP1
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig GLP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 80-93  
    1:2 Average % 95  
    Range % 90-105  
    1:4 Average % 98  
    Range % 92-110  
    1:8 Average % 93  
    Range % 85-98  
  • 回收率：
    The recovery of pig GLP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-104  
    EDTA plasma (n=4) 94 90-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.028 2.014 2.021 1.803  
    500 1.562 1.540 1.551 1.333  
    250 0.949 0.932 0.941 0.723  
    125 0.565 0.568 0.567 0.349  
    62.5 0.351 0.347 0.349 0.131  
    31.2 0.301 0.322 0.312 0.094  
    15.6 0.284 0.286 0.285 0.067  
    0 0.214 0.222 0.218 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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問答及客戶評論

■ 常見問題解答
Q:

I want to measure total GLP-1 in plasma samples, but I do not understand how it would be biologically relevant to measure 1-37 (with no cross reactivity to other fragments) since GLP-1 is released from the L-cells to the blood stream as either 7-37 or 7-36 and then rapidly degraded to 9-37 and 9-36.
As far as I understand 1-37 is not present in plasma. Could you please explain?

A:
Thanks for your mail!
For the in-body enviroment, the half life of GLP-1 is around 30 seconds. Then GLP-1 cannot be detected in this enviroment.
When the sample prepared and detached from the original in-body enviroment, it can be detected in this condition.
As for the different fragments, it is validated from our side that there is no across activity with another peptide fragments.
Q:

I was wondering if you knew whether the "Pig glucagon like peptide 1(GLP1) ELISA Kit" is meant for pig serum/plasma, or human serum/plasma?

A:
Thanks for your inquir! Pig glucagon like peptide 1(GLP1) ELISA KitCSB-EQ027281PI
This kit is specific to pig. It is suitable to test pig serum/plasma.