Pig hemoglobin (Hb) ELISA Kit

說(shuō)明書(shū)下載

產(chǎn)品詳情

產(chǎn)品描述：

This Pig hemoglobin (Hb) ELISA Kit was designed for the quantitative measurement of Pig hemoglobin (Hb) protein in serum, plasma, lysate for RBC.. It is a Competitive ELISA kit, its detection range is 0.625 μg/mL-40 μg/mL and the sensitivity is 0.312 μg/mL.
縮寫(xiě)：

Hb
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, lysate for RBC.
檢測(cè)范圍：

0.625 μg/mL-40 μg/mL
靈敏度：

0.312 μg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig Hb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-105
1:2	Average %	92
	Range %	86-100
1:4	Average %	95
	Range %	89-99
1:8	Average %	95
	Range %	87-105

回收率：

The recovery of pig Hb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-99
Plasma (n=5)	96	90-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.